转杂色鲍抗冻肽-Ⅲ基因表达载体pIRES2-EGFP-AFP-HdiActinP的构建

现代转基因技术是一种快速改良品种特性的有效方法.采用转基因技术提高我国南方重要贝类经济养殖品种--杂色鲍的耐低温特性是扩大其养殖范围的重要手段.以pIRES2-EGFP为基本载体,以合成并经过功能鉴定的抗冻肽(AFP)和杂色鲍肌动蛋白启动子(HdiActinP)为插入元件,首先用限制性内切酶BamHⅠ和 EcoRⅠ对基本载体pIRES2-EGFP和AFP-Ⅲ进行双酶切,目的酶切产物被回收后再连接得到载体pIRES2-EGFP-AFP.随后利用限制性内切酶SacⅠ和 EcoRⅠ对载体pIRES2-EGFP-AFP和HdiActinP进行双酶切,目的酶切产物被回收后再连接最终得到杂色鲍转AFP-Ⅲ的表达载体pIRES2-EGFP-AFP-HdiActinP,经过聚合酶链式反应和测序验证证明载体构建成功.     

Recognition of Chinese Organization Name Using Co-training

Chinese organization name recognition is hard and important in natural language processing. To reduce tagged corpus and use untagged corpus, we presented combing Co-trainins with support vector machines (SVM) and conditional random fields (CRF) to improve recognition results. Based on principles of uncorrelated and compatible, we constructed different classifiers from different views within SVM or CRF alone and combination of these two models. And we modified a heuristic untagged samples selection algorithm to reduce time complexity. Experimental results show that under the same tagged data, Co-training has 10% F-measure higher than using SVM or CRF alone; under the same F-measure, Co-training saves at most 70% of tagged data to achieve the same performance.     

对含宏观大裂纹圆柱壳的轴压临界载荷的分析

采用动力学有限元法研究了带裂纹圆柱壳两种裂纹长度和十种裂纹方向的临界载荷，计算结果表明，在不同裂纹长度下，裂纹方向对圆柱壳的轴压屈曲强度有较大影响。     

基于扩展数据包络判别法的商业银行信用风险评估

考虑到我国商业银行样本数据分布的非正态性和多维性特点,文章将扩展的数据包络判别模型引入商业银行信用风险评估中.该模型具有非参数检验特性,通过两阶段分类过程对企业信用状况进行判别.实证结果表明,与传统的Logistic分析模型相比,扩展数据包络判别模型的预测精度更好.     

药用植物染料的特征和功能实现(Ⅱ):染色效果及抗菌性能

介绍了常用天然植物药的染色效果和实现条件，及其药用植物染料的上染特征，染色牢度的特点和提高染色效果的方法，并概述了织物经药用植物染料染色后的抗微生物活性和抗紫外线功能。     

Erlang-平稳二项过程模型结构可靠度一致最小方差无偏估计

讨论结构在设计基准期[O,T]内应力变动规律,考虑应力为平稳二项过程、强度服从Erlang分布的半随机过程模型,给出应力随机过程的样本函数的最大值分布,并获得其相应的模型结构可靠度最小方差无偏估计.     

Murine arylsulfatase C: Evidence for two isozymes

Summary SWR/J mice posses high arylsulfatase C, estrone sulfatase, and dehydroepiandrosterone sulfatase activities in liver, spleen and kidney compared to A/J mice. This internstrain activity variation appears to be determined by at least 1 autosomal gene. Murine arylsulfatase C activity occurs in both hydrophobic and hydrophilic forms which differ with respect to certain biochemical properties and exhibit different subcellular distributions. The hydrophilic isozyme is a major component in kidney and brain extracts and a minor isozyme in liver and spleen extracts. The hydrophobic arylsulfatase C isozyme appears to be identical to steroid sulfatase. The hydrophilic arylsulfatase C isozyme does not possess steroid sulfatase activity; however, hydrophilic and hydrophobic arylsulfatase C share certain properties, suggesting that they may be structurally related. The autosomal gene(s) affects both arylsulfatase isozymes.This research was supported in part by National Institutes of Health grant GM 27707.     

中美社会力量设立科学技术奖比较研究

对中美两国社会力量设奖的历史与现状进行了系统考察,通过对比分析,认为二者在4个方面有相似之处,包括设奖者以专业学会为主、奖励范围以单个学科为主、奖励对象以个人为主、物质奖励和精神奖励相结合等;同时也存在5个方面的主要区别,包括社会力量设奖的影响力、国际奖的数量、对社会力量设奖的管理、资金来源、评审机制等。在此基础上,对如何推动中国社会力量设奖的健康发展提出了看法。     

皖西大别山区人地关系综合评价

为了协调皖西大别山区人地关系,在建立区内人地关系综合评价模型的基础上,对区内人地关系进行了综合评价,评价结果值为５９．７根据评价因子的匹配系数（Ｃｃ）,发现区域开发中资源条件和环境条件较好,但社会问题和经济问题比较突出,对此提出稳定和持续发展区内人地关系的对策,加皖西大别山区经济的发展．