Fractalkine基因真核表达质粒的构建及其在小鼠肝癌细胞中的表达

为克隆小鼠趋化因子Fractalkine(．FK)基因，构建真核表达质粒，并在小鼠肝癌细胞中表达，用以进行肿瘤的基因治疗，用RT-PCR法，从小鼠乳腺癌细胞D2F2扩增FK的cDNA，插入pCR2．1 TOPO载体，测序证实后，将其亚克隆至质粒pIRES中构建FK真核表达载体；用脂质体将重组质粒转染小鼠肝癌MM45 T．Li细胞，经G418筛选获得抗性细胞克隆，用RT-PCR和免疫化学方法鉴定转染细胞中FK基因的表达．结果表明：经限制性内切酶酶切图谱分析和DNA序列测定证实目的基因已插入重组质粒，RT-PCR和免疫化学方法证明转基因MM45T．Li细胞克隆中存在小鼠FK基因的表达。     

铁纳米线阵列的结构与微观磁性

采用电化学方法制备了平行排列的Fe纳米线阵列。利用透射电镜、X—射线衍射、物性测量系统和穆斯堡尔谱分别对样品的形貌、晶体结构、宏观磁性和微观磁性进行了研究。结果表明样品形状均匀，但沿纳米线方向有晶体织构，且存在着很强的形状各向异性。利用结果，结合计算机模拟，对样品中原子磁矩的分布进行了讨论。     

进位反馈移位寄存器状态图分布的进一步研究

进一步研究了以奇数q为连接数的FCSR的所有q 1个周期状态的状态图的圈长和计数问题，使得FCSR的状态图分布与LFSR的状态图分布一样清晰。     

基于消息中间件的网络协同编辑环境框架

给出了利用消息中间件和事件抽象技术来实现文本协同编辑环境的实现框架 ,并在此框架的基础上实现了基于MBus中间件的共享WPS协同编辑系统 .基于此框架可以在网络多媒体会议系统中实现各种可编辑文档的共享和协同编辑 .此框架通过实现的协同文本编辑环境验证了实现网络会议系统中各种可编辑文档协同共享编辑的可能性和可靠性 ,并且能够进一步完善视频会议的功能并增加会议的真实感和沉浸感     

滨南油田低渗欠注井酸化增注技术研究与应用

提出了一种针对滨南低渗油田欠注井酸化增注的系统实验研究方法。根据储层特征分析了注污水和重复酸化对地层的损害及对酸化液的选取原则。根据长岩心酸化流动模拟实验进行了酸化工艺参数优选及其评估分析。现场施工证明该酸化液体系适应性较好 ,工艺参数合理 ,能够较好地解除地层中有机物和无机物堵塞 ,提高注水量 ,降低泵注压力 ,在低渗油田注水井中具有推广价值。     

Emergent Property in Macromolecular Motion

In this paper, the model of inverse cascade fractal super-blocks along one direction (in the positive or negative) in the 3-dimensional space is developed to describe the self-similar motion in macromolecular system. Microscopically the cohesive and dispersed states of the motion blocks are co- existent states with vastly different probability of occurrence. Experimental results and theoretical analysis show that the microscopic cohesive state energy and dispersed state energy of each motion block are respectively equal to the macroscopic glassy state energy kTg and molten state energy kTm of the system. This singularity unveils topologically the non-integrability, mathematically the anholonomy, and macroscopically the emergent property. This singularity also reveals that the glass, viscoelastic and melt states are three distinct emergent properties of macromolecular motion from a macroscopic viewpoint. The fractal concept of excluded volume is introduced to depict the random motion at various scales in     

Effect of Carboxymethyl Starch as Stabilizer for Increasing the Stability of Warp Sizing Pastes

The influences of carboxymethyl starch used as stabilizer upon the stability of native cornstarch-polyvinyl alcohol blend pastes for warp sizing have been investigated. The effect of the modified starch on the paste stability was evaluated in terms of the initial demixing time and the volume percentage of separated starch. The carboxymethyl starch with a series of different degrees of substitution was prepared in ethanol dispersion by varying the amount of monochloroacetic acid reacted with refined native cornstarch. The paste stability strongly depends on the modification extent and amount of carboxymethyl starch used, and on native starch content in the paste. Increase in the modification extent and/or the amount of the modified starch effectively retards the phase separation and reduces the separation extent of native cornstarch-polyvinyl alcohol blend pastes. Moreover, the mechanism and favorable modification extent of carboxymethyl starch for enhancing paste stability are also investigated and disc     

Research on Precision Assembly Robot''''s Joint Torque Control Based on Current Measurement

A set of new current sensing device is used to realize joint torque control based on current measurement in a precision assembly robot's third joint. The output torque's model of the joint's brushless DC motor is founded. Disturbance factors and the compensated effect of the torque's closed loop based on current measurement are analyzed. Related simulations and experiments show that the system has good current tracking and anti-disturbances performance, which improve the force control performance of the robot in assembly.     

Open Contours Extraction of Rotational Surface Oriented to Layer Measurement

With layer-measured contours, an algorithm that can extract the contour segments from a rotational surface is presented. The extraction can be divided into two stages, i. e. the rough segmentation and the refinement. In the rough segmenting stage, an optimal contour matching method is put forward to find similar contour segment from another closed contour with respect to the seed contour. In the refining stage, an iterative way that can extract a circular arc precisely is presented based on parameters identification and contour-ends expanding/shrinking operation. The algorithm can extract the open contour segments from a rotational surface precisely, as demonstrated in the examples. Based on the work of this paper, further research, such as parameter identification of 3 - D surface and CAD model creation, can be conducted.     

Novel Hyper-Redundant Manipulator-. Design, Study and Experiment

A novel hyper-redundant manipulator named RT1 is designed and studied. The unique feature of RT1 is all degrees of freedom (DOF) are actuated with only one motor via special designed hinge bar universal joints. The mechanisms of RT1 are introduced in detail. Some experiments are carried out in order to test the movability and adaptability of the manipulator. RT1 is actuated by pulse string and acts discretely. The discrete working space of RT1 is described and the parameter optimization for kinematical redundancy resolution is studied also. The optimization criterion is altering the design parameter as little as possible during manipulator's motion from the initial position to the expected position. An optimization example is given that is realized with Matlab optimize tool-box.