蛋白聚糖NG2中硫酸软骨素糖胺聚糖链调节稳定转染NG2的U251细胞迁移能力

应用PCR反应使编码蛋白聚糖NG2的核苷酸在3064-3065位置的AG突变成为GC, 构建突变型NG2/S999A. 用表达野生型NG2和突变型NG2/S999A及空白表达载体分别稳定转染人成胶质细胞瘤U251. 应用Western Blot方法鉴定转染后的U251细胞表达野生型及突变型NG2的状态, 证实突变型NG2A999稳定转染的U251细胞株表达的NG2缺失硫酸软骨素葡糖胺聚糖链（C S-GAG）. 比较了3种U251细胞株的迁移, 表明蛋白聚糖NG2中CS-GAG链影响细胞的迁移能 力.     

Genetic variation in the gene encoding calpain-10 is associated with type 2 diabetes mellitus

Type 2 or non-insulin-dependent diabetes mellitus (NIDDM) is the most common form of diabetes worldwide, affecting approximately 4% of the world's adult population. It is multifactorial in origin with both genetic and environmental factors contributing to its development. A genome-wide screen for type 2 diabetes genes carried out in Mexican Americans localized a susceptibility gene, designated NIDDM1, to chromosome 2. Here we describe the positional cloning of a gene located in the NIDDM1 region that shows association with type 2 diabetes in Mexican Americans and a Northern European population from the Botnia region of Finland. This putative diabetes-susceptibility gene encodes a ubiquitously expressed member of the calpain-like cysteine protease family, calpain-10 (CAPN10). This finding suggests a novel pathway that may contribute to the development of type 2 diabetes.     

iASPP oncoprotein is a key inhibitor of p53 conserved from worm to human

We have previously shown that ASPP1 and ASPP2 are specific activators of p53; one mechanism by which wild-type p53 is tolerated in human breast carcinomas is through loss of ASPP activity. We have further shown that 53BP2, which corresponds to a C-terminal fragment of ASPP2, acts as a dominant negative inhibitor of p53 (ref. 1). Hence, an inhibitory form of ASPP resembling 53BP2 could allow cells to bypass the tumor-suppressor functions of p53 and the ASPP proteins. Here, we characterize such a protein, iASPP (inhibitory member of the ASPP family), encoded by PPP1R13L in humans and ape-1 in Caenorhabditis elegans. iASPP is an evolutionarily conserved inhibitor of p53; inhibition of iASPP by RNA-mediated interference or antisense RNA in C. elegans or human cells, respectively, induces p53-dependent apoptosis. Moreover, iASPP is an oncoprotein that cooperates with Ras, E1A and E7, but not mutant p53, to transform cells in vitro. Increased expression of iASPP also confers resistance to ultraviolet radiation and to cisplatin-induced apoptosis. iASPP expression is upregulated in human breast carcinomas expressing wild-type p53 and normal levels of ASPP. Inhibition of iASPP could provide an important new strategy for treating tumors expressing wild-type p53.     

牦牛骨胶原蛋白肽的制备及其抗冻性能研究

以新鲜牦牛骨为原料,制备了牦牛骨胶原抗冻肽,并对其抗冻性能进行了研究。结果表明,以EDTA·2Na为脱钙剂对牛骨进行脱钙的最佳脱钙浓度为0.25 mol/L,脱钙时长为48 h,得到的胶原蛋白最大提取率为43.99%。胃蛋白酶酶解制备酶解复合物的最佳时间为48 h,温度25 ℃,得到的胶原蛋白的提取率可达48.53%。同时,采用改良的冰亲和吸附法对胶原抗冻肽进行吸附,得到最佳吸附时间为4 h,吸附温度为-2 ℃,吸附浓度为2 mg/ml,在此条件下,蛋白得率为48.1%,而热滞活性值可高达5.76 ℃。牦牛骨胶原抗冻肽属于一种高热滞活性蛋白资源,在食品冷冻保藏以及运输等领域有较好的应用前景。     

外商直接投资对新疆经济增长影响的实证分析

随着改革开放不断深入,新疆投资环境的改善,外商直接投资迅速增加,而变成为新疆经济增长的主要因素。本文运用协整分析、格兰杰因果关系检验,外商直接投资与新疆经济增长的关系上进行实证分析,因而提出建议。     

2N＋1阶KdV型方程的Adomian近似解析解

利用Adomian分解法,给出2N+1阶KdV型方程的近似解析解,将Adomian近似解与精确解进行比较,结果表明,近似解具有很高的精确度,收敛于精确解的速度也很快.     

新疆金发藓科的新记录属——仙鹤藓属

金发藓科(Polytrichaceae)目前全世界共17属.我国有6属54种,新疆有4属12种.其中仙鹤藓属在全世界有20种,中国有6种,1变种.通过利用扫描电镜法对仙鹤藓属植物的孢子和蒴齿进行微形态观察,利用徒手切片法对其叶片、茎进行切割并在光学显微镜下观察其形态结构并照相,结果发现从新疆天山西部地区特克斯县琼库什台和巩留县塔里木采集的3份标本具有相同的特征,并与文献进行比较后发现其特征与小胞仙鹤藓[Atrichum rhystopphyllum( Müll. Hal.) Par.]一致,确定为小胞仙鹤藓.最主要的特征是叶腹面栉片4～6列,多数高2～5个细胞.仙鹤藓属和小胞仙鹤藓是新疆首次发现的新记录属、新记录种.本文提供了小胞仙鹤藓的详细的形态描述及图版,并提供了新疆金发藓科植物的分属检索表;该种的发现为新疆金发藓科的研究提供了新的资料.     

一类变系数Boussinesq方程的相似约化解

运用CK直接约化法对一类描述方向上存在可变剪切流动的长波变系数Boussinesq方程进行相似约化,可以得到原方程的一些相似变换和相似解.在已有文献的基础上,用CK直接约化法进一步讨论了变系数Boussinesq方程的相似约化问题,得到了几种新的相似解.     

光纤中变系数非线性Schrdinger方程的精确类孤子解

利用一种函数变换,将光纤中变系数非线性Schrdinger方程约化为非线性常微分方程.通过求解非线性常微分方程,获得了光纤中变系数非线性Schrdinger方程的精确类孤子解.这种方法也可用于其他非线性方程,如变系数Kp方程、带强迫项变系数组合KdV方程等.     

Sophisticated particle-feeding in a large Early Cambrian crustacean

Most Cambrian arthropods employed simple feeding mechanisms requiring only low degrees of appendage differentiation. In contrast, post-Cambrian crustaceans exhibit a wide diversity of feeding specializations and possess a vast ecological repertoire. Crustaceans are evident in the Cambrian fossil record, but have hitherto been known exclusively from small individuals with limited appendage differentiation. Here we describe a sophisticated feeding apparatus from an Early Cambrian arthropod that had a body length of several centimetres. Details of the mouthparts resolve this taxon as a probable crown-group (pan)crustacean, while its feeding style, which allowed it to generate and handle fine food particles, significantly expands the known ecological capabilities of Cambrian arthropods. This Early Cambrian record predates the major expansions of large-bodied, particle-handling crustaceans by at least one hundred million years, emphasizing the importance of ecological context in driving adaptive radiations.