Redox modulated hydrogelation of a self-assembling short peptide amphiphile

Hydrogels resulting from the self-assembly of small peptides are smart nanobiomaterials as their nanostructuring can be readily tuned by environmental stimuli such as pH,ionic strength and temperature,thereby favoring their practical applications.This work reports experimental observations of formation of peptide hydrogels in response to the redox environment.Ac-I 3 K-NH 2 is a short peptide amphiphile that readily self-assembles into long nanofibers and its gel formation occurs at concentrations of about 10 mmol/L.Introduction of a Cys residue into the hydrophilic region leads to a new molecule,Ac-I 3 CGK-NH 2,that enables the formation of disulfide bonds between self-assembled nanofibers,thus favoring cross-linking and promoting hydrogel formation.Under oxidative environment,Ac-I 3 CGK-NH 2 formed hydrogels at much lower concentrations(even at 0.5 mmol/L).Furthermore,the strength of the hydrogels could be easily tuned by switching between oxidative and reductive conditions and time.However,AFM,TEM,and CD measurements revealed little morphological and structural changes at molecular and nano dimensions,showing no apparent influence arising from the disulfide bond formation.     

新型竹—木—GFRP夹层梁的受弯性能试验

为研究竹—木—GFRP夹层梁的受弯性能,设计以泡桐木作为芯材,竹、GFRP作为面层的夹层梁试件,对夹层梁试件进行了等芯材厚度和等梁高两组四点弯静载试验,得出各试件的破坏现象、破坏荷载并绘制荷载—位移曲线。研究结果表明:竹材部分替代GFRP作为面层的一部分,可降低成本,防止加载点处局部破坏;设置泡桐木纤维沿梁长度方向有利于提高夹层梁的受弯性能;芯材厚度不变的情况下,竹材加固夹层梁有极高的性价比,而使用GFRP面层则能显著提高夹层梁的弯曲刚度;梁高不变的情况下,夹层梁的弯曲刚度、极限荷载分别随竹材与GFRP厚度的增加而增大。竹—木—GFRP夹层梁跨中截面应变分布基本满足平截面假定。换算截面法可作为竹—木—GFRP夹层梁应力计算依据,使用考虑剪切变形的铁木辛柯梁理论计算竹—木—GFRP夹层梁的跨中挠度有着不错的精度。     

人工站与自动站气温观测数据的对比分析

根据长沙2003年人工站和自动站的气温观测数据,分析其对比差值,发现:(1)长沙年平均气温对比差值为-0.17,即自动站的气温平均比人工站高0.17℃.对于短期气候预测,长沙自动站的气温观测数据是可用的.就气候研究来说,长沙自动站的气温观测数据是不可用的;(2)在一年四季中,长沙秋季和冬季的对比差值的绝对值都小于0.2.因而此时长沙自动站的气温观测数据可信且可用;春季和夏季的对比差值的绝对值都大于等于0.2,因而长沙自动站这两季的气温观测数据是不可靠的,必须慎重使用;(3)在一天中,长沙晚上和夜间的对比差值的绝对值都很小,因而自动站的观测数据是完全可信的;清晨和中午的对比差值的绝对值都比较大,因而此时的 自动站数据不宜使用;(4)造成对比差值偏大的原因主要是自动站的仪器对冷空气活动和突然变化的天气不够敏感.因为对比差值的绝对值偏大的时候,都是冷空气活动的前后.     

英文词汇的近形检索和通配查找

是对一个课程设计实践的小结,讨论了在电子词典中含一定模糊度和近似性的查找所采用的算法,面向这种方法的词汇表的组织和依据;叙述了所采用的软件工程的设计方法     

恒加试验随机截尾指数分布数据加速系数的LSE

针对恒定应力加速寿命试验下随机截尾指数寿命数据进行统计分析。在加速寿命方程未知的情况下 ,给出了加速系数的近似最小二乘估计方法。随后的随机模拟实例表明 :所给的统计方法计算精度较高     

带有参数的数据包络分析C~2R模型

本文为了改进C~2R模型反映决策单元相对信息不完全的不足,提出了参数型数据包络分析模型,讨论了这个模型的有关性质,给出了阔值和有效值的概念.经过理论分析和实例验证,这两个值可更好地反映决策单元的相对信息.     

基于Oracle Spatial空间数据共享系统设计

近年来，随着面向对象数据库技术成熟，使用大型的RDBMS管理海量空间数据成为可能，企业化GIS数据组织和处理就显得非常必要。空间数据共享是当前地理信息系统建设的重要课题，而空间数据转换是实现信息共享的重要途径。本文研究当前流行空间数据共享方案，设计一种以XML为信息交换标准的空间数据转换系统，将空间数据接口统一集成到0racleSpatia1中。此设计方案已用于数字重庆(DCQGIS)建设项目。     

Construction and anti-tumor effects of recombinant fowlpox virus expressing Newcastle disease virus hemagglutinin-neuramidinase gene

Hemagglutinin-neuramidinase (HN), a Newcastle disease virus-derived protein, not only mediates receptor recognition but also possesses neuraminidase (NA) activity, the ability to cleave a component of those receptors, N-acetylneuraminic acid (NAcneu, sialic acid). It is known that this protein in mammalian species, including human beings, has interesting anti-neoplastic as well as immune stimulating properties. To explore the use of the HN gene in cancer gene therapy, we constructed a recombi-nant fowlpox virus expressing the HN protein (vFV-HN) and compared the anti-tumor activity of the recombinant virus with that of wild-type fowlpox virus (FPV) in vivo and in vitro. Here we found that although B16 cells were somewhat resistant to the basal cytotoxic effect of wild-type fowlpox virus, infection with vFV-HN caused a pronounced cytotoxic effect and, the survival of tumor-bearing mice immunized with vFV-HN was significantly increased compared with the survival of mice immunized with the FPV alone. Furthermore, the immunization of mice with vFV-HN elicited a B16 tumor-specific cytotoxic T lymphocyte (CTL) response and clonal expansion of both CD4 and CD8 T cell populations in vivo. In addition, T cells from lymph nodes of mice vaccinated with vFV-HN secreted high levels of the Th1 cytokine IL-2 and IFN-γ, indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with vFV-HN may be a potential strategy for cancer gene therapy.     

区域土壤墒情模型研究

运用平原水文模型,参考不同作物不同生长期蒸散发特点及土层结构和土层蓄水量与土壤墒情之间的关系,构建区域土壤墒情模型,并利用山西省洪洞县实测土壤墒情资料对该模型进行了检验,检验结果表明,模型物理概念明确,可连续模拟或预报区域土壤墒情,且预报精度高。     

锂离子电池和双电层电容器用LiODFB-TEABF4复合盐电解液的研究

探讨在EC+PC+DMC复合溶剂体系中LiODFB-TEABF4复合盐电解液与LiFePO4锂离子电池及AC双电层电容器的相容性规律.研究结果表明:在LiODFB基电解液中加入TEABF4能显著提高电解液的电导率;对于LiFePO4电池体系,电解液中的TEABF4参与了SEI膜的成膜过程,但TEABF4浓度过高不利于电极材料的容量的提高;对于AC电容器体系,加入TEABF4可以有效改善电容器的双电层储能行为,同时显著提高电容,当TEABF4浓度为0.3 mol/L时,电容达到最大,比不添加TEABF4的纯LiODFB盐电解液的电容大.