Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry

A library of 50,000 recombinants representative of the human X chromosome has been constructed. Human X chromosomes were physically separated using a fluorescence-activated cell sorter. The DNA was purified from the chromosomes, digested to completion with the restriction enzyme EcoRI and cloned into the phage lambda gtWES.lambda B. The X-derived nature of the recombinants was confirmed by hybridization to rodent/human cell line DNA containing only the human X chromosome. Such libraries will be particularly useful for the investigation of genetic diseases such as Duchenne muscular dystrophy, where the basic defect has not been elucidated, and of neoplasia, where several specific chromosomal anomalies, particularly for the leukaemias, have been identified.     

多元Kantorovi型Meyer-Knig-Zeller算子在Orlicz空间中的逼近阶

在Orlicz空间L*M中研究二元Kantorovi型Meyer-K nig-Zeller算子的逼近,给出一种逼近强型正定理.     

大型水利枢纽砼运输浇筑系统的GPSS模型及仿真研究

本文应用系统仿真原理和GPSS(General Purpose Simulation System——通用仿真系统)仿真语言构造了大型水利枢纽混凝土工程的运输浇筑系统的GPSS模型，并通过计算机运行对该系统进行了仿真研究，得出了具有一定实用价值的结果，为大型水利枢纽的可行性研究、设计、施工提供了参考．     

断层相互作用产生同震库仑应力改变分布图像

讨论了同震库仑应力改变的基本含义,以及基于静应力位错理论求解同震库仑应力改变的边界元方法,对与发震断层相互平行和相互垂直两种典型构造模式下,断层相互作用产生的同震库仑应力改变进行了数值模拟研究,分别获取了相应条件下的同震库仑应力改变空间分布图像.结果表明:不同构造模式的断层相互作用,其产生的同震库仑应力改变分布图像存在较大差异,在分析和评价断层相互作用产生的同震库仑应力改变,及其对活动断层地震危险性的影响时,应该充分考虑待评价断层与发震断层之间的空间几何关系、断层的性质和产状等特定的构造条件.     

利用V-I曲线分析负荷扰动对电压稳定性的影响

建立了电力系统供应侧和负荷侧V -I静特性的数学模型 ,提出了利用特性曲线来判断电压稳定性的方法 ,并利用负荷特性曲线的变化趋势来分析负荷增长对电压稳定性的影响。该方法简单、实用 ,可应用于实时在线电压稳定分析     

Thermal effect of the cable-stayed bridge tower

0　IntroductionXiantaoHanjiangRiverHighwayBridgelocatesonnorth westsuburbofXiantaocity ,crossesovertheHanjiangRiv er.Thecable stayedbridgeisasingle towersystemwiththespansof (5 0 + 82 + 180 )m .Thenon symmetricalstructureisusedheresothatthemainpiercankeepoffthemainchannel.Thesectionofthegirderisnotconstantinorderthatthedeadloadcanbeinbalancealthoughthespansaredifferent.Thedeckwidthis 2 3m (notincludingthewidthofthecablelane) ,laterallayoutisthatofdouble directionfour lanewithoutsidewalk ,b…     

A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage

The p53 gene is frequently inactivated in human cancers. Here we have isolated a p53-inducible gene, p53R2, by using differential display to examine messenger RNAs in a cancer-derived human cell line carrying a highly regulated wild-type p53 expression system. p53R2 contains a p53-binding sequence in intron 1 and encodes a 351-amino-acid peptide with striking similarity to the ribonucleotide reductase small subunit (R2), which is important in DNA synthesis during cell division. Expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wild-type p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and prevented cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that have an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair and cell survival after exposure to various genotoxins. Our results indicate that p53R2 encodes a ribonucleotide reductase that is directly involved in the p53 checkpoint for repair of damaged DNA. The discovery of p53R2 clarifies a relationship between a ribonucleotide reductase activity involved in repair of damaged DNA and tumour suppression by p53.     

提高底部框架——抗震墙结构综合抗震能力的措施

文章分析了底部框架--抗震墙结构在地震震害中的破坏特征,论述了合理确定抗震墙间距、数量及分布和控制层间刚度比的必要性,提出了在底部框架--抗震墙结构设计应该注意的问题,并结合工程实例作了具体分析.     

Nogo-66 receptor antagonist peptide promotes axonal regeneration

Myelin-derived axon outgrowth inhibitors, such as Nogo, may account for the lack of axonal regeneration in the central nervous system (CNS) after trauma in adult mammals. A 66-residue domain of Nogo (Nogo-66) is expressed on the surface of oligodendrocytes and can inhibit axonal outgrowth through an axonal Nogo-66 receptor (NgR). The IN-1 monoclonal antibody recognizes Nogo-A and promotes corticospinal tract regeneration and locomotor recovery; however, the undefined nature of the IN-1 epitope in Nogo, the limited specificity of IN-1 for Nogo, and nonspecific anti-myelin effects have prevented a firm conclusion about the role of Nogo-66 or NgR. Here, we identify competitive antagonists of NgR derived from amino-terminal peptide fragments of Nogo-66. The Nogo-66(1 40) antagonist peptide (NEP1 40) blocks Nogo-66 or CNS myelin inhibition of axonal outgrowth in vitro, demonstrating that NgR mediates a significant portion of axonal outgrowth inhibition by myelin. Intrathecal administration of NEP1 40 to rats with mid-thoracic spinal cord hemisection results in significant axon growth of the corticospinal tract, and improves functional recovery. Thus, Nogo-66 and NgR have central roles in limiting axonal regeneration after CNS injury, and NEP1-40 provides a potential therapeutic agent.     

钼酸盐-柠檬酸盐络合物及有机酸对棕色固氮菌生长的影响

将经过0.9%NaCl溶液处理8h的棕色固氮菌(AzotobactervinelandiiOP)作为菌种分别接入Burk′s培养基和用不同有机酸替代柠檬酸三钠或用不同等摩尔的钼络合物替代钼酸钠的各种改良的Burk′s培养基中,分别测定菌体生长曲线和固氮活性.结果发现,与Burk′s培养基相比,以高柠檬酸、苹果酸、马来酸替代柠檬酸三钠或以K6[Mo2O5(cit)2]·5H2O、K4[Mo2O5(Hcit)2]·4H2O和Na2[MoO2(Hcit)]·3H2O替代柠檬酸三钠和钼酸钠的培养基能促进菌体的生长;以乙醇酸替代柠檬酸三钠的培养基则会抑制固氮菌的生长;各种培养条件下菌体细胞的C2H2还原活性表现了类似的规律.讨论了固氮酶活性中心FeMoco在装配过程中钼的可能运输方式和装配机理.