Monolayer cultures of perikarya isolated from postnatal rat cerebellum

Summary Viable cerebellar perikarya of mixed cell type obtained from 7–9-day-old rats were maintained in monolayer culture for up to 12 days. During this time extensive neurite formation and outgrowth occurred. The large majority of the cells developing in culture were tentatively identified as granule neurons. This identification was based on the large number isolated from the starting tissue, and the cells general morphological features in culture such as perikaryal and nuclear size, the bipolar nature of neurite extension, and their migratory behaviour.D.N. Currie was supported by a grant from Hoechst Pharmaceuticals, Ld, U.K.J.C. was supported by an S.R.C. Grant to S.P.R. RoseThe authors would like to thank Kathleen Tear for excellent technical assistance and members of the Brain Research Group for helpful discussions.     

Lysergic acid diethylamide affects blood flow to specific areas of the conscious rat brain

Zusammenfassung Die i.v. Verabreichung von LSD an Ratten steigert den Blutkreislauf im Hirnstamm sowie im frontalen und parietalen Cortex selektiv. Es wurden durch 6-Nor-LSD, 2-Methyl-LSD und ^{8, 9}-LSD weder wahrnehmbare Verhaltensänderungen noch Änderungen des regionalen Blutkreislaufs verursacht.     

Struktur-Wirkungsbeziehungen beim menschlichen Calcitonin. III. Die biologische Aktivität verkürzter oder an den Kettenenden modifizierter,synthetischer analoger

Summary Assays of 8 synthetic analogues of human calcitonin in rats showed that their hypocalcaemic activity was drastically reduced by deletion of the C-terminal amide group, chain-shortening or opening of the disulphide ring, but unaffected or enhanced by modification of the N-terminal amino group.

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II. vgl.R. Maier, B. Kamber, B. Riniker andW. Rittel, Hormon. Metab. Res.7, 511 (1975).

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Die hier verwendete, abgekürzte Schreibweise für Peptide folgt den Empfehlungen der IUPAC-IUB Kommission für Biochemische Nomenklatur, J.B.C.247, 977 (1972). Weitere Abkürzungen: HCT, Human-Calcitonin; PCT, Schweinecalcitonin; Bmp, -Mercaptopropionsäure (Desaminocystein); NMet, Normethionin (S-Methylcystein); -OMe, Methylester.     

Ecdysone 20-hydroxylase from the midgut of the tobacco hornworm (Manduca sexta L.)

Summary An ecdysone 20-hydroxylase enzyme system that converts -ecdysone to 20-hydroxyecdysone was prepared from the midgut of the tobacco hornworm prepupa. This partially purified enzyme is NADPH dependent and is localized in the mitochondrial fraction of the midgut tissue.