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Doxorubicin (DXR) (0.17 x 10(-4) M) induces an acute cardiotoxicity in isolated rat heart; there is a progressive widening of the S alpha T segment, with a decrease in force derivatives and in the coronary flow. Concurrent perfusion with fructose-1,6-diphosphate (FDP) (10(-5)-10(-4) M) dose-dependently reduces the S alpha T enlargement but fails to affect the reduction in force derivatives and coronary flow. The target of cardiac protection by FDP might be the ionic mechanisms underlying the action potential configuration. 相似文献
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N. Ishii 《Cellular and molecular life sciences : CMLS》1986,42(7):810-812
Summary The maximal unloaded shortening velocity (Vmax) of smooth muscle cells isolated from the pedal retractor muscle ofMytilus was more than twice as large as that of the whole muscle, suggesting the presence of extracellular components which resist the contraction of the whole muscle. The Vmax of the isolated cells was almost constant at cell lengths ranging between 0.5 and 0.8310 (10, optimal length for tension generation) indicating that the intracellular resistance to contraction is negligible within this range of lengths. 相似文献
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Cloning and expression in E. coli of the malarial sporozoite surface antigen gene from Plasmodium knowlesi 总被引:14,自引:0,他引:14
J Ellis L S Ozaki R W Gwadz A H Cochrane V Nussenzweig R S Nussenzweig G N Godson 《Nature》1983,302(5908):536-538
The malarial sporozoite, the infective stage found in the salivary gland of the insect vector, bears highly immunogenic surface antigen(s). Repeated exposure to irradiated sporozoites induces protection against malaria in several host species, including man. Further, monoclonal antibodies that confer passive immunity react with the immunogenic surface determinants of different sporozoite species. One approach to prevent malaria, therefore, would be to produce a vaccine that induces high titres of circulating antibodies against the sporozoite surface determinant(s). However, production of such a vaccine has not been possible since sporozoites cannot be cultivated in vitro and, therefore, only limited amounts of surface antigen may be obtained. To overcome this problem, we have prepared mRNA from Plasmodium knowlesi-infected mosquitoes to construct a cDNA library. From this library we have isolated a clone that expresses the sporozoite surface antigen as a beta-lactamase fusion protein in the plasmid pBR322. This is the first potentially protective malarial antigen to be cloned by recombinant DNA technology. 相似文献
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