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161.
MicroRNAs (miRNAs) are short ~21-nt non-coding RNA molecules that have been shown to regulate a number of biological processes.
Previous reports have shown that overexpression of miR-128 in glioma cells inhibited cell proliferation. Literature also suggests
that miR-128 negatively regulates prostate cancer cell invasion. Here, we show that overexpression of hsa-miR-128, a brain-enriched
microRNA, induces apoptosis in HEK293T cells as elucidated by apoptosis assay, cell cycle changes, loss of mitochondrial membrane
potential and multicaspase assay. By in silico analysis, we identified a putative target site within the 3′ untranslated region
(UTR) of Bax, a proapoptotic member of the apoptosis pathway. We found that ectopic expression of hsa-miR-128 suppressed a
luciferase reporter containing the Bax-3′ UTR and reduced the levels of Bax in HEK293T cells. Taken together, our study demonstrates
that overexpression of hsa-miR-128 not only induces apoptosis in HEK293T cells but also is an endogenous regulator of Bax
protein. 相似文献
162.
Translation initiation is a critical step in protein synthesis. Previously, two major mechanisms of initiation were considered
as essential: prokaryotic, based on SD interaction; and eukaryotic, requiring cap structure and ribosomal scanning. Although
discovered decades ago, cap-independent translation has recently been acknowledged as a widely spread mechanism in viruses,
which may take place in some cellular mRNA translations. Moreover, it has become evident that translation can be initiated
on the leaderless mRNA in all three domains of life. New findings demonstrate that other distinguishable types of initiation
exist, including SD-independent in Bacteria and Archaea, and various modifications of 5′ end-dependent and internal initiation
mechanisms in Eukarya. Since translation initiation has developed through the loss, acquisition, and modification of functional
elements, all of which have been elevated by competition with viral translation in a large number of organisms of different
complexity, more variation in initiation mechanisms can be anticipated. 相似文献
163.
Montuori N Bifulco K Carriero MV La Penna C Visconte V Alfano D Pesapane A Rossi FW Salzano S Rossi G Ragno P 《Cellular and molecular life sciences : CMLS》2011,68(14):2453-2467
The receptor (CXCR4) for the stromal-derived factor-1 (SDF1) and the urokinase-receptor (uPAR) are up-regulated in various tumors. We show that CXCR4-transfected cells migrate toward SDF1 on collagen (CG) and do not on vitronectin (VN). Co-expression of cell-surface uPAR, which is a VN receptor, impairs SDF1-induced migration on CG and allows migration on VN. Blocking fMLP receptors (fMLP-R), alpha-v integrins or the uPAR region capable to interact with fMLP-Rs, impairs migration of uPAR/CXCR4-transfected cells on VN and restores their migration on CG. uPAR co-expression also reduces the adherence of CXCR4-expressing cells to various components of the extracellular matrix (ECM) and influences the partitioning of beta1 and alpha-v integrins to membrane lipid-rafts, affecting ECM-dependent signaling. uPAR interference in CXCR4 activity has been confirmed in cells from prostate carcinoma. Our results demonstrate that uPAR expression regulates the adhesive and migratory ability of CXCR4-expressing cells through a mechanism involving fMLP receptors and alpha-v integrins. 相似文献
164.
Lubka-Pathak M Shah AA Gallozzi M Müller M Zimmermann U Löwenheim H Pfister M Knipper M Blin N Schimmang T 《Cellular and molecular life sciences : CMLS》2011,68(16):2739-2749
Introduction
Tff3 peptide exerts important functions in cytoprotection and restitution of the gastrointestinal (GI) tract epithelia. Moreover, its presence in the rodent inner ear and involvement in the hearing process was demonstrated recently. However, its role in the auditory system still remains elusive. Our previous results showed a deterioration of hearing with age in Tff3-deficient animals.Results
Present detailed analysis of auditory brain stem response (ABR) measurements and immunohistochemical study of selected functional proteins indicated a normal function and phenotype of the cochlea in Tff3 mutants. However, a microarray-based screening of tissue derived from the auditory central nervous system revealed an alteration of securin (Pttg1) and serpina3n expression between wild-type and Tff3 knock-out animals. This was confirmed by qRT-PCR, immunostaining and western blots.Conclusions
We found highly down-regulated Pttg1 and up-regulated serpina3n expression as a consequence of genetically deleting Tff3 in mice, indicating a potential role of these factors during the development of presbyacusis. 相似文献165.
Effectiveness of immune surveillance of intracellular viruses and bacteria depends upon a functioning antigen presentation
pathway that allows infected cells to reveal the presence of an intracellular pathogen. The antigen presentation pathway uses
virtually all endogenous polypeptides as a source to produce antigenic peptides that are eventually chaperoned to the cell
surface by MHC class I molecules. Intriguingly, MHC I molecules present peptides encoded not only in the primary open reading
frames but also those encoded in alternate reading frames. Here, we review recent studies on the generation of cryptic pMHC
I. We focus on the immunological significance of cryptic pMHC I, and the novel translational mechanisms that allow production
of these antigenic peptides from unconventional sources. 相似文献
166.
帕力哈提.米吉提 《新疆大学学报(自然科学维文版)》2012,(2):45-51
稳频的连续飞秒脉冲激光器以狭窄的光学共振作为水准基点,这种激光器具有稳定频率成分的宽带光谱和大功率的超短脉冲,能够有效的快速转变频率。在本文中可以看出这种双光子吸收共振使氢原子1S-2S能级之间跃迁的钛宝石激光器稳频飞秒脉冲振荡机制。 相似文献
167.
S.S.吴 《国外科技新书评介》2009,(5)
Gabor分析和小波分析在信号分析、图像处理和其它信息科学领域有着广泛的应用,二者都适合描述时频信号的局部特征,与传统的Fourier分析描述的稳定信号相比它们具有极大的灵活性和实用性。 相似文献
168.
M.哈尔哈里 《国外科技新书评介》2009,(5)
非交换几何对于大多数数学家来讲,是一个陌生的概念。它不仅是当代数学的最前沿,而且涉及所有布尔巴基数学的交叉,即使这些数学也不是简单的:测度论、代数拓扑学、算子代数、微分拓扑学、微分(黎曼)几何学……,更让数学家头痛的还包括整套的量子物理学。 相似文献
169.
本书是施普林格出版社的光学科学系列丛书的136卷,作者佩波宁教授于1977年、1979年分别在约恩苏大学获得学士和硕士学位,1982年在约恩苏大学获得博士学位,此后一直在约恩苏大学从事教学和科研工作,长期从事光学测量方面的研究工作。 相似文献
170.
本文应用数量化理论,给出了如何建立煤层注水效果的预测数学模型,并结合实例,进行了预测.实例表明,根据本矿已知煤层注水工程的各种信息,采用数量化理论.建立的计算机预测煤层注水效果的方法,不仅可以确切地判定所给定的注水组合参数是否最优,同时还揭露了注水参数与水分增值的内在关系,为调控参数提供了科学手段 相似文献