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621.
Determination of surface topography of biological specimens at high resolution by scanning tunnelling microscopy 总被引:1,自引:0,他引:1
A M Baró R Miranda J Alamán N García G Binnig H Rohrer C Gerber J L Carrascosa 《Nature》1985,315(6016):253-254
Although techniques are available for the determination of the three-dimensional structure of biological specimens, for example scanning electron microscopy, they all have some serious drawback, such as low resolution, the requirement for crystals or for the sample to be analysed in a high vacuum. In an attempt to develop a technique for high-resolution three-dimensional structure analysis of non-crystalline biological material, we have tested the applicability of scanning tunnelling microscopy (STM), a method that has been used successfully in the analysis of metal and semiconductor surface structures. We report here that scanning tunnelling electron microscopy can be used to determine the surface topography of biological specimens at atmospheric pressure and room temperature, giving a vertical resolution of the order of 1 A. Our results show that quantum mechanical tunnelling of electrons through biological material is possible provided that the specimen is deposited on a conducting surface. 相似文献
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Organization of microtubules in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau 总被引:35,自引:0,他引:35
Here we report that the microtubule-associated proteins MAP2 and tau share two separable functional domains. One is the microtubule-binding site which serves to nucleate microtubule assembly; the second is a short C-terminal alpha-helical sequence which can crosslink microtubules by means of a hydrophobic zipper interaction into dense stable parallel arrays characteristic of axons or dendrites. Thus, interactions between molecules of a single type are capable of drastically reorganizing microtubules and completely suppressing their dynamic properties. 相似文献
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C N Gillis 《Experientia》1971,27(11):1317-1319
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M N Islam 《Experientia》1978,34(2):254-255
Antibodies against nucleotide-peptides of beef heart do crossreact with nucleotide-peptides of other beef organs, but with nucleotide-peptides of Ehrlich tumor cells. Antibodies against the latter do not crossreact with nucleotide-peptides of normal organs, but do so with nucleotide-peptides of a rat hepatoma. 相似文献