Maintenance of human embryonic stem cells on gelatin

Matrigel is routinely used as a coating material in the feeder-free culture system of human embryonic stem cells （hESCs）. However, matrigel is costive and inconvenient to use. In this study, the possibility of using gelatin as an alternative coating material was investigated. The results showed that, after trypsinization, hESCs were maintained undifferentiated on gelatin. These hESCs expressed pluripotent markers, formed teratoma and maintained a normal karyotype. As measured at passage 10, the hESCs expressed a high level of Oct4 on both gelatin and Matrigeh hESCs growing on gelatin formed AP-positive colonies in similar size and number to those growing on Matrigel （P〉 0.05）. Moreover, hESCs growing on gelatin contained a comparable percentage of SSEA-4-positive cells to those growing on Matrigel （95.1% vs.94.3%, P〉 0.05）. H-1 hESCs were maintained undifferentiated on gelatin for 20 passages and remained the stable normal karyotype. This gelatin-based culture protocol may allow us to propagate hESCs in large scale, with less cost.     

二阶拟线性微分方程的振动准则

对二阶拟线性微分方程[r（t）｜x’（t）｜^α-1x’（t）]’＋p（t）｜x’（t）｜α-1x’（t）＋g（t）｜x（t）｜^β-1x（t）=0,利用积分平均法和黎卡提变换技巧,得到了一些新的振动准则,改进和推广了Kamenev、Philos、Wang、Xu的结果．     

负重轮橡胶轮缘有限元分析及力学特性拟合

采用均匀设计法,选取履带车辆负重轮橡胶轮缘的２４组不同几何参数,建立橡胶轮缘的三维有限元分析模型,用ＡＮＳＹＳ有限元分析软件计算橡胶轮缘的位移,应力和应变分布情况,最后对橡胶轮缘的最大径向位移,应力和应变进行多元线性回归。得到了橡胶轮缘的最大径向位移,应力和应变随７个影响因素的拟合特性,试验验证表明,所得拟合公式有实用价值。     

调控酶解底物初始pH制备低聚木糖

研究在木聚糖酶定向酶解纯木聚糖及木聚糖碱抽提液制备功能性低聚木糖时,酸和缓冲溶液调控底物的初始ｐＨ对低聚木糖的影响。结果表明,木聚糖酶在ｐＨ５．０左右的活力最高;酸调控纯木聚糖底物的低聚木糖得率低于缓冲溶液调控的低聚木糖得率,但两者相关不大;而当底物为木聚糖碱抽提液是,由于木素的存在,两种调控方式的低聚木糖得率相差较大,酶解液出现浑浊状态;酸调控纯木聚糖,木聚糖碱抽提液时,低聚木糖得率最高的初始ｐ     

复合岩石损伤本构方程与实验

大量沉积岩样的电镜与岩相微观研究表明,岩石是由含不同形态的晶体和孔隙构成,由此建立岩石微结构损伤模型。进而导出了各自损伤演化过程、力学参数、有效应力、不等围压等因素的多层复合岩石三维非线性损伤本构方程及损伤演化方程。实验结果表明,二者吻合良好。     

分形图像压缩方法的改进与实现

提出了一种降低分形图像压缩方法计算复杂度的改进方法,该方法已在VC＋＋5．0集成开发环境下得以实现,并取得较好的实验效果。     

耳萝卜螺胚胎发育的初步观察

采用定时取样 ,间断观察的方法 ,对耳萝卜螺胚胎发育的形态特征进行了观察。发现耳萝卜螺的胚胎发育过程分 7个时期 :卵裂期、囊胚期、原肠期、担轮幼虫期、面盘幼虫期、幼螺形成期和孵化期。对各期的外部形态特征进行了描述 ,并绘制了各期形态图 ,记录了各期发育所经历的时间     

小型农用运输车驾驶平顺性分析

为适应当今对农用车舒适性及环保的要求 ,本文就路面不平度造成驾驶座椅垂直振动这一影响驾驶舒适性的主要因素进行分析 ,建立了振动模型 ,并对结构参数进行优化计算 ,结果可与国际标准ISO2 631进行比较评介。     

Identification of common variants associated with human hippocampal and intracranial volumes

Identifying genetic variants influencing human brain structures may reveal new biological mechanisms underlying cognition and neuropsychiatric illness. The volume of the hippocampus is a biomarker of incipient Alzheimer's disease and is reduced in schizophrenia, major depression and mesial temporal lobe epilepsy. Whereas many brain imaging phenotypes are highly heritable, identifying and replicating genetic influences has been difficult, as small effects and the high costs of magnetic resonance imaging (MRI) have led to underpowered studies. Here we report genome-wide association meta-analyses and replication for mean bilateral hippocampal, total brain and intracranial volumes from a large multinational consortium. The intergenic variant rs7294919 was associated with hippocampal volume (12q24.22; N = 21,151; P = 6.70 × 10(-16)) and the expression levels of the positional candidate gene TESC in brain tissue. Additionally, rs10784502, located within HMGA2, was associated with intracranial volume (12q14.3; N = 15,782; P = 1.12 × 10(-12)). We also identified a suggestive association with total brain volume at rs10494373 within DDR2 (1q23.3; N = 6,500; P = 5.81 × 10(-7)).     

有关流形间泊松映射的几点讨论

本文建立了一个与泊松映射有关的交换图表,在此基础之上讨论了泊松映射的几个性质,进而说明了泊松映射可以把泊松流形上的余迷向子流形映为余迷向子流形．最后,还得到了泊松流形上的Casimir函数在泊松同构的作用下仍是Casimir函数的结论．