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A series of reciprocating wear tests were performed on the deep cryogenically treated and conventionally heat-treated samples of 100Cr6 bearing steel to study the wear resistance. The worn surfaces as well as the wear debris were analyzed by scanning electron microscopy. The improvement in wear resistance of the deep cryogenically treated samples ranges from 49% to 52%. This significant improvement in wear resistance can be attributed to finer carbide precipitation in the tempered martensitic matrix and the transformation of retained austenite into martensite. X-ray diffraction analysis shows that the volume fraction of retained austenite in the conventionally heat-treated samples is 14% and that of the deep cryogenically treated samples is only 3%. 相似文献
63.
砂体非线性分布的定量研究方法 总被引:5,自引:1,他引:4
介绍了分形几何学和分形地质统计学,并以大庆榆树林油田的砂体精细定量描述为例,利用分形地质统计学方法,对大庆榆树林油田砂体的分形分布进行了定量表征,为油田开发中的砂体非线性分布的数学定量表征提供了一种比较实用和可行的方法. 相似文献
64.
Huber C Dias-Santagata D Glaser A O'Sullivan J Brauner R Wu K Xu X Pearce K Wang R Uzielli ML Dagoneau N Chemaitilly W Superti-Furga A Dos Santos H Mégarbané A Morin G Gillessen-Kaesbach G Hennekam R Van der Burgt I Black GC Clayton PE Read A Le Merrer M Scambler PJ Munnich A Pan ZQ Winter R Cormier-Daire V 《Nature genetics》2005,37(10):1119-1124
Intrauterine growth retardation is caused by maternal, fetal or placental factors that result in impaired endovascular trophoblast invasion and reduced placental perfusion. Although various causes of intrauterine growth retardation have been identified, most cases remain unexplained. Studying 29 families with 3-M syndrome (OMIM 273750), an autosomal recessive condition characterized by severe pre- and postnatal growth retardation, we first mapped the underlying gene to chromosome 6p21.1 and then identified 25 distinct mutations in the gene cullin 7 (CUL7). CUL7 assembles an E3 ubiquitin ligase complex containing Skp1, Fbx29 (also called Fbw8) and ROC1 and promotes ubiquitination. Using deletion analysis, we found that CUL7 uses its central region to interact with the Skp1-Fbx29 heterodimer. Functional studies indicated that the 3-M-associated CUL7 nonsense and missense mutations R1445X and H1464P, respectively, render CUL7 deficient in recruiting ROC1. These results suggest that impaired ubiquitination may have a role in the pathogenesis of intrauterine growth retardation in humans. 相似文献
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66.
Li W Moore MJ Vasilieva N Sui J Wong SK Berne MA Somasundaran M Sullivan JL Luzuriaga K Greenough TC Choe H Farzan M 《Nature》2003,426(6965):450-454
Spike (S) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (SARS), associate with cellular receptors to mediate infection of their target cells. Here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ACE2), isolated from SARS coronavirus (SARS-CoV)-permissive Vero E6 cells, that efficiently binds the S1 domain of the SARS-CoV S protein. We found that a soluble form of ACE2, but not of the related enzyme ACE1, blocked association of the S1 domain with Vero E6 cells. 293T cells transfected with ACE2, but not those transfected with human immunodeficiency virus-1 receptors, formed multinucleated syncytia with cells expressing S protein. Furthermore, SARS-CoV replicated efficiently on ACE2-transfected but not mock-transfected 293T cells. Finally, anti-ACE2 but not anti-ACE1 antibody blocked viral replication on Vero E6 cells. Together our data indicate that ACE2 is a functional receptor for SARS-CoV. 相似文献
67.
Summary A new rapid micromethod for protein separation under a radial electric field is described. As many as 12 rabbit serum samples could be separated in 4–6 min.The authors are thankful to Dr P.K. Ramachandran, Defence Research and Development Establishment, Gwalior, for the sustained interest and helpful suggestions. 相似文献
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69.
Résumé Les semences deDrosophyllum lusitanicum Link, cultivées en milieu semi-solide de White (WB) et également dans des milieux auxquels on a ajouté de nombreux facteurs de croissance, n'ont pas germé, mais les embryons incisés ont commencé à germer tout de suite et ils se sont développés lorsqu'on les a semés sur le WB, et sur le WB + CH + IAA ou le WB + kinetin ou encore sur le WB + l'extrait de levure. Sur le WB + CH + CM, une callosité friable s'est produite. Celle-ci a été maintenue à travers plusieurs passages au cours de 18 mois. Une différentiation en pousses et racines a été observée dans 12% des tissus de calles sous-cultivés. 相似文献