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151.
152.
Release of invasive plants from fungal and viral pathogens 总被引:71,自引:0,他引:71
Invasive plant species both threaten native biodiversity and are economically costly, but only a few naturalized species become pests. Here we report broad, quantitative support for two long-standing hypotheses that explain why only some naturalized species have large impacts. The enemy release hypothesis argues that invaders' impacts result from reduced natural enemy attack. The biotic resistance hypothesis argues that interactions with native species, including natural enemies, limit invaders' impacts. We tested these hypotheses for viruses and for rust, smut and powdery mildew fungi that infect 473 plant species naturalized to the United States from Europe. On average, 84% fewer fungi and 24% fewer virus species infect each plant species in its naturalized range than in its native range. In addition, invasive plant species that are more completely released from pathogens are more widely reported as harmful invaders of both agricultural and natural ecosystems. Together, these results strongly support the enemy release hypothesis. Among noxious agricultural weeds, species accumulating more pathogens in their naturalized range are less widely noxious, supporting the biotic resistance hypothesis. Our results indicate that invasive plants' impacts may be a function of both release from and accumulation of natural enemies, including pathogens. 相似文献
153.
Climate change: Regional warming and malaria resurgence 总被引:1,自引:0,他引:1
Patz JA Hulme M Rosenzweig C Mitchell TD Goldberg RA Githeko AK Lele S McMichael AJ Le Sueur D 《Nature》2002,420(6916):627-8; discussion 628
154.
Dragon F Gallagher JE Compagnone-Post PA Mitchell BM Porwancher KA Wehner KA Wormsley S Settlage RE Shabanowitz J Osheim Y Beyer AL Hunt DF Baserga SJ 《Nature》2002,417(6892):967-970
Although the U3 small nucleolar RNA (snoRNA), a member of the box C/D class of snoRNAs, was identified with the spliceosomal small nuclear RNAs (snRNAs) over 30 years ago, its function and its associated protein components have remained more elusive. The U3 snoRNA is ubiquitous in eukaryotes and is required for nucleolar processing of pre-18S ribosomal RNA in all organisms where it has been tested. Biochemical and genetic analyses suggest that U3 pre-rRNA base-pairing interactions mediate endonucleolytic pre-rRNA cleavages. Here we have purified a large ribonucleoprotein (RNP) complex from Saccharomyces cerevisiae that contains the U3 snoRNA and 28 proteins. Seventeen new proteins (Utp1 17) and Rrp5 were present, as were ten known components. The Utp proteins are nucleolar and specifically associated with the U3 snoRNA. Depletion of the Utp proteins impedes production of the 18S rRNA, indicating that they are part of the active pre-rRNA processing complex. On the basis of its large size (80S; calculated relative molecular mass of at least 2,200,000) and function, this complex may correspond to the terminal knobs present at the 5' ends of nascent pre-rRNAs. We have termed this large RNP the small subunit (SSU) processome. 相似文献
155.
156.
Mary R. Mitchell B. L. Ginsborg C. R. House 《Cellular and molecular life sciences : CMLS》1980,36(1):98-99
Summary After incubation in calcium-free solutions containing cobalt, the readmission of calcium caused prolonged but reversible hyperpolarization of acinar cells of cockroach salivary glands and prolonged fluid secretion. It is suggested that cobalt treatment increases the permeability of the acinar cell membrane to calcium.Acknowledgments. We are indebted to the Medical Research Council for support. 相似文献
157.
158.
J A Camacho C Obie B Biery B K Goodman C A Hu S Almashanu G Steel R Casey M Lambert G A Mitchell D Valle 《Nature genetics》1999,22(2):151-158
Neurospora crassa ARG13 and Saccharomyces cerevisiae ARG11 encode mitochondrial carrier family (MCF) proteins that transport ornithine across the mitochondrial inner membrane. We used their sequences to identify EST candidates that partially encode orthologous mammalian transporters. We thereby identified such a gene (ORNT1) that maps to 13q14 and whose expression, similar to that of other urea cycle (UC) components, was high in liver and varied with changes in dietary protein. ORNT1 expression restores ornithine metabolism in fibroblasts from patients with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome. In a survey of 11 HHH probands, we identified 3 ORNT1 mutant alleles that account for 21 of 22 possible mutant ORNT1 genes in our patients: F188delta, which is common in French-Canadian HHH patients and encodes an unstable protein; E180K, which encodes a stable, properly targeted protein that is inactive; and a 13q14 microdeletion. Our results show that ORNT1 encodes the mitochondrial ornithine transporter involved in UC function and is defective in HHH syndrome. 相似文献
159.
Bacteriophage lambda has for many years been a model system for understanding mechanisms of gene regulation. A 'genetic switch' enables the phage to transition from lysogenic growth to lytic development when triggered by specific environmental conditions. The key component of the switch is the cI repressor, which binds to two sets of three operator sites on the lambda chromosome that are separated by about 2,400 base pairs (bp). A hallmark of the lambda system is the pairwise cooperativity of repressor binding. In the absence of detailed structural information, it has been difficult to understand fully how repressor molecules establish the cooperativity complex. Here we present the X-ray crystal structure of the intact lambda cI repressor dimer bound to a DNA operator site. The structure of the repressor, determined by multiple isomorphous replacement methods, reveals an unusual overall architecture that allows it to adopt a conformation that appears to facilitate pairwise cooperative binding to adjacent operator sites. 相似文献
160.
Putting endotoxin to work for us: Monophosphoryl lipid A as a safe and effective vaccine adjuvant 总被引:2,自引:0,他引:2
The development of non-infectious subunit vaccines greatly increases the safety of prophylactic immunization, but also reinforces
the need for a new generation of immunostimulatory adjuvants. Because adverse effects are a paramount concern in prophylactic
immunization, few new adjuvants have received approval for use anywhere in the developed world. The vaccine adjuvant monophosphoryl
lipid A is a detoxified form of the endotoxin lipopolysaccharide, and is among the first of a new generation of Toll-like
receptor agonists likely to be used as vaccine adjuvants on a mass scale in human populations. Much remains to be learned
about this compound’s mechanism of action, but recent developments have made clear that it is unlikely to be simply a weak
version of lipopolysaccharide. Instead, monophosphoryl lipid A’s structure seems to have fortuitously retained several functions
needed for stimulation of adaptive immune responses, while shedding those associated with pro-inflammatory side effects.
Received 25 April 2008; received after revision 05 June 2008; accepted 10 June 2008 相似文献