Isolation and identification of the three rice monotelosomics

In order to obtain rice monotelosomic, the progeny of 24 telotrisomics, derived from an indica rice variety, Zhongxian 3037, were screened. The variants that differed morphologically from the diploids and the original primary trisomics as well as the telotrisomics were collected for cytological identification. The variants with 24 chromosomes were selected according to the prometaphase chromosomes. From these variants, three monotelosomies with one chromosome arm deletion in each were verified by fluorescence in situ hybridization （FISH） using a rice centromeric BAC clone of 17p22 as a marker probe. The three monotelosomics were derived from telotrisomic 1S, 4L and 11L, respectively. Further identification was conducted on the prometaphase or pachytene chromosomes of the three variants, which were probed with the same centromeric BAC clone together with the corresponding chromosome arm specific makers, a0059H02 （on the short arm of chromosome 1）, a0034E24 （on the long arm of chromosome 4）, and a0071H11 （on the long arm of chromosome 11）. The results indicated that the telocentric chromosomes in the three monotelosom. ics were derived from their respective corresponding telotrisomics. According to the telocentric chromosomes of the variants, they were monotelosomic 1S （one long arm of chromosome 1 was lost）, monotelosomic 4L （one short arm of chromosome 4 was lost） and monotelosomic 11L （one short arm of chromosome 11 was lost）, respectively.     

马尾松种源对松材线虫病的抗性

通过１９８９￣１９９７年对马尾松（Ｐｉｎｕｓ ｍａｓｓｏｎｉａｎａ Ｌａｍｂ）种源对松材线虫病的抗性选育，发现７￣１０年生各种源均抗权材线虫病（Ｂｕｒｓａｐｈｅｌｅｎｃｈｕｓ ｘｙｌｏｐｈｉｌｕｓ （Ｓｔｅｉｎｅｒ ＆ Ｂｕｈｒｅｒ）Ｎｉｃｋｌｅ），１２年生后的不同年龄和不同马尾松种源的抗性差异很大，在４０个马尾松种源中抗性稳定（ＩＲ＝１００）的有３个（广西２号，广西３号和广东５号）。经模糊聚类分     

电视频道的形象包装与宣传

介绍了电视频道形象包装中台标、宣传片、标语、节目主持人的重要性,提出在形象包装中要注重各方面技术的综合运用,才能提高社会声誉,扩大社会影响,吸引观众。     

上海中心城区道路灰尘中氯代多环芳烃的时空分布特征

选取了上海中心城区的道路灰尘作为研究对象, 运用气相色谱-质谱(gas chromatography-mass spectrometry, GC/MS)联用技术对道路灰尘中20种氯代多环芳烃(chlorinated polycyclic aromatic hydrocarbons, ClPAHs)进行了检测, 分析了道路灰尘中ClPAHs的时空分布特征和毒性当量(toxic equivalent quantity, TEQ)等. 结果表明, 11种ClPAHs的检出率均大于50%, 说明ClPAHs在上海中心城区道路灰尘中普遍存在. 研究区域内的ClPAHs浓度呈现出明显的季节特征, 即冬季>夏季, 冬季∑ClPAHs浓度最高为24.9 ng/g干重. 在空间分布上, 交通枢纽区浓度最高(冬季为21.2 ng/g干重, 夏季为10.7 ng/g干重), 建筑施工区、商业区次之, 近绿地区最低. 在道路灰尘中最主要的两个组分是6-ClBaP和7-ClBaA. ClPAHs在冬季和夏季的TEQ范围分别为0.01~4.81和0.01~1.78 ng-TEQ/g干重, 其中 7-ClBaA在ClPAHs总毒性当量中的贡献最大.     

Generation of selectable marker-free transgenic rice resistant to chewing insects using two co-transformation systems

To produce selectable marker-free (SMF) transgenic rice resistant to chewing insects, the Bacillus thuringiensis cryIA(c) gene (Bt) was introduced into two elite japonica rice varieties by using two Agrobacterium-mediated co-transformation systems. One system is with a single mini-twin T-DNA binary vector in one Agrobacterium strain, which consists of two separate T-DNA regions, one carrying the Bt while the other contains the selectable marker gene, hygromycin resistant gene (HPT). The other system uses two separate binary vectors in two separate Agrobacterium cultures, containing the Bt or HPT gene on individual plasmids. A lot of independent transgenic rice lines harboring both Bt and selectable marker genes were obtained. The results showed that the co-transformation frequency of the Bt gene and HPT gene was much higher by using the mini-twin T-DNA vector system (29.87%) than that by the two separate binary vector systems (4.52%). However, the frequency of the SMF transgenic rice plants obtained from the offspring of co-transgenic plants (21.74%) was lower for the mini-twin T-DNA vector system than that for the latter (50-60%). The data of ELISA implied that the expressed Bt proteins were quantitated as 0.025-0.103% of total leaf soluble proteins in the transgenic plant. Therefore, several elite transgenic rice lines, free of the selectable marker gene, were chosen. The results from both in vitro and in vivo insect bioassays indicated that the SMF transgenic rice was shown to be highly resistant to the striped stem borer and rice leaf folder. Moreover, in a natural field condition without any insecticide applied, all the transgenic rice plants were found to be not injured by the rice leaf folder, whereas the wild types were impaired seriously.     

基于VisualFoxPro环境开发管理系统

VisualFoxPro作为关系型数据库管理系统及应用软件开发工具,既符合数据库系统的开发要求,又具有可视化编程的优势。其某些特殊对象的建立有一定的通用性,而它的项目管理器能起到组织文件及编译发布应用程序的作用。     

光学活性姜烯类倍半萜的合成及其抗生育活性的探索

本文描述了具有光学活性的姜烯类倍半萜化合物——(-)姜烯、(-)姜烯醇和(-)β-倍半水芹烯的合成。讨论了2-甲基6-(3-乙酰基-环丁-2-烯基-1)-2-庚烯的结构。药理初筛结果表明都具有一定的抗生育活性,其中(-)姜烯的抗生育活性令人满意。     

建筑工程预算定额的应用技巧

在编制单位工程施工图预算时，除了要准确计算工程量之外，还需要综合、正确、灵活地应用各种预算定额及相关的配套手册，才能最终确定工程造价。本文紧密结合工作实践，对我省现行建筑工程预算定额的应用进行了一些探索和总结，对于提高工作效率，减少差错具有一定的实际价值。可作为编制建筑工程概预算时的参考。     

低秩先验引导的无监督域自适应行人重识别

无监督域自适应行人重识别在智能监控中发挥着重要作用,并引起了研究者的广泛关注.尽管目前的研究已经取得了较大进步,但不同数据集之间的域偏移问题给行人重识别带来极大挑战.研究发现,在连续时间里,同一摄像机视角下的行人图像具有相同的风格,如果将这种风格信息从行人图像中分离出去,将有效缓解由图像风格差异引起的域偏移问题.为此,提出一种低秩先验引导的域不变信息分离的字典学习方案.根据风格信息的低秩先验性,将行人图像特征中的风格信息和行人身份信息分离开来,根据同一身份行人属性的域不变性建立视觉特征与属性之间的联系,缓解域偏移所带来的影响,通过自训练策略来调整学习参数.实验表明,方法的性能在很多数据集上超过了传统的无监督域自适应行人重识别方法以及部分基于深度学习的无监督域自适应行人重识别方法.