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排序方式: 共有125条查询结果,搜索用时 203 毫秒
71.
Schmutz J Wheeler J Grimwood J Dickson M Yang J Caoile C Bajorek E Black S Chan YM Denys M Escobar J Flowers D Fotopulos D Garcia C Gomez M Gonzales E Haydu L Lopez F Ramirez L Retterer J Rodriguez A Rogers S Salazar A Tsai M Myers RM 《Nature》2004,429(6990):365-368
As the final sequencing of the human genome has now been completed, we present the results of the largest examination of the quality of the finished DNA sequence. The completed study covers the major contributing sequencing centres and is based on a rigorous combination of laboratory experiments and computational analysis. 相似文献
72.
A stable binary quasicrystal 总被引:1,自引:0,他引:1
73.
Zhou X Gomez-Smith M Qin Z Duquette PM Cardenas-Blanco A Rai PS Harper ME Tsai EC Anisman H Chen HH 《Cellular and molecular life sciences : CMLS》2012,69(5):819-828
The LIM domain only 4 (LMO4) protein is expressed in the hypothalamus, but its function there is not known. Using mice with
LMO4 ablated in postnatal glutamatergic neurons, including most neurons of the paraventricular (PVN) and ventromedial (VMH)
hypothalamic nuclei where LMO4 is expressed, we asked whether LMO4 is required for metabolic homeostasis. LMO4 mutant mice
exhibited early onset adiposity. These mice had reduced energy expenditure and impaired thermogenesis together with reduced
sympathetic outflow to adipose tissues. The peptide hormone leptin, produced from adipocytes, activates Jak/Stat3 signaling
at the hypothalamus to control food intake, energy expenditure, and fat metabolism. Intracerebroventricular infusion of leptin
suppressed feeding similarly in LMO4 mutant and control mice. However, leptin-induced fat loss was impaired and activation
of Stat3 in the VMH was blunted in these mice. Thus, our study identifies LMO4 as a novel modulator of leptin function in
selective hypothalamic nuclei to regulate fat metabolism. 相似文献
74.
Cho YS Chen CH Hu C Long J Ong RT Sim X Takeuchi F Wu Y Go MJ Yamauchi T Chang YC Kwak SH Ma RC Yamamoto K Adair LS Aung T Cai Q Chang LC Chen YT Gao Y Hu FB Kim HL Kim S Kim YJ Lee JJ Lee NR Li Y Liu JJ Lu W Nakamura J Nakashima E Ng DP Tay WT Tsai FJ Wong TY Yokota M Zheng W Zhang R Wang C So WY Ohnaka K Ikegami H Hara K Cho YM Cho NH Chang TJ Bao Y Hedman ÅK Morris AP McCarthy MI;DIAGRAM Consortium;MuTHER Consortium Takayanagi R Park KS Jia W Chuang LM Chan JC Maeda S Kadowaki T Lee JY Wu JY 《Nature genetics》2012,44(1):67-72
We conducted a three-stage genetic study to identify susceptibility loci for type 2 diabetes (T2D) in east Asian populations. We followed our stage 1 meta-analysis of eight T2D genome-wide association studies (6,952 cases with T2D and 11,865 controls) with a stage 2 in silico replication analysis (5,843 cases and 4,574 controls) and a stage 3 de novo replication analysis (12,284 cases and 13,172 controls). The combined analysis identified eight new T2D loci reaching genome-wide significance, which mapped in or near GLIS3, PEPD, FITM2-R3HDML-HNF4A, KCNK16, MAEA, GCC1-PAX4, PSMD6 and ZFAND3. GLIS3, which is involved in pancreatic beta cell development and insulin gene expression, is known for its association with fasting glucose levels. The evidence of an association with T2D for PEPD and HNF4A has been shown in previous studies. KCNK16 may regulate glucose-dependent insulin secretion in the pancreas. These findings, derived from an east Asian population, provide new perspectives on the etiology of T2D. 相似文献
75.
Amos CI Wu X Broderick P Gorlov IP Gu J Eisen T Dong Q Zhang Q Gu X Vijayakrishnan J Sullivan K Matakidou A Wang Y Mills G Doheny K Tsai YY Chen WV Shete S Spitz MR Houlston RS 《Nature genetics》2008,40(5):616-622
To identify risk variants for lung cancer, we conducted a multistage genome-wide association study. In the discovery phase, we analyzed 315,450 tagging SNPs in 1,154 current and former (ever) smoking cases of European ancestry and 1,137 frequency-matched, ever-smoking controls from Houston, Texas. For replication, we evaluated the ten SNPs most significantly associated with lung cancer in an additional 711 cases and 632 controls from Texas and 2,013 cases and 3,062 controls from the UK. Two SNPs, rs1051730 and rs8034191, mapping to a region of strong linkage disequilibrium within 15q25.1 containing PSMA4 and the nicotinic acetylcholine receptor subunit genes CHRNA3 and CHRNA5, were significantly associated with risk in both replication sets. Combined analysis yielded odds ratios of 1.32 (P < 1 x 10(-17)) for both SNPs. Haplotype analysis was consistent with there being a single risk variant in this region. We conclude that variation in a region of 15q25.1 containing nicotinic acetylcholine receptors genes contributes to lung cancer risk. 相似文献
76.
77.
利用酚羟基封端的聚砜和二氯封端、含Si-H功能键的硅氧烷低聚体合成交替偶联嵌段共聚物。由于Si-H键在碱性介质中不稳定,二氯封端的聚硅氢烷被选作嵌段基元.所得聚合物用IR、H ̄1NMR、GPC和TEM作了表征,结果表明嵌段合成是成功的.通过Si-H键与烯键的加成反应在该共聚物的分子链上引入了碱性侧基而得到一系列嵌段型高分子配体. 相似文献
78.
Systemic Practice and Action Research - Aesthetic education is currently an educational issue receiving substantial attention, and to increase the accessibility of aesthetic education, working to... 相似文献
79.
Lin SM Tsai JY Hsiao CD Huang YT Chiu CL Liu MH Tung JY Liu TH Pan RL Sun YJ 《Nature》2012,484(7394):399-403
H(+)-translocating pyrophosphatases (H(+)-PPases) are active proton transporters that establish a proton gradient across the endomembrane by means of pyrophosphate (PP(i)) hydrolysis. H(+)-PPases are found primarily as homodimers in the vacuolar membrane of plants and the plasma membrane of several protozoa and prokaryotes. The three-dimensional structure and detailed mechanisms underlying the enzymatic and proton translocation reactions of H(+)-PPases are unclear. Here we report the crystal structure of a Vigna radiata H(+)-PPase (VrH(+)-PPase) in complex with a non-hydrolysable substrate analogue, imidodiphosphate (IDP), at 2.35?? resolution. Each VrH(+)-PPase subunit consists of an integral membrane domain formed by 16 transmembrane helices. IDP is bound in the cytosolic region of each subunit and trapped by numerous charged residues and five Mg(2+) ions. A previously undescribed proton translocation pathway is formed by six core transmembrane helices. Proton pumping can be initialized by PP(i) hydrolysis, and H(+) is then transported into the vacuolar lumen through a pathway consisting of Arg?242, Asp?294, Lys?742 and Glu?301. We propose a working model of the mechanism for the coupling between proton pumping and PP(i) hydrolysis by H(+)-PPases. 相似文献
80.