Lysosomal chymotrypsin B potentiates apoptosis via cleavage of Bid

We have reported that chymotrypsin B (CtrB) is not just a digestive enzyme but is also stored in lysosomes. Herein, we demonstrated a broad distribution of CtrB and explored the involvement of CtrB in apoptosis. Exposure of RH-35 cells to H₂O₂ or palmitate induced the redistribution of lysosomal CtrB into the cytoplasm as a result of lysosomal membrane permeabilization (LMP). Suppression of CtrB significantly blocked apoptosis, while overexpression of CtrB sensitized apoptosis markedly. CtrB could cleave Bid under neutral conditions. In RH-35 cells with Bid silenced, apoptosis induced by CtrB protein was attenuated, suggesting that CtrB mediates apoptosis of RH-35 cells mainly through processing Bid. Our data also suggest that LMP occurs earlier than mitochondrial outer membrane permeabilization; Bid activation initiated by caspase-8 might be reinforced by CtrB in consequence of LMP, which causes a positive feedback loop leading to the accumulation of tBid, and results in lysosome- and mitochondrion-dependent apoptosis.     

基于动态语义服务描述的服务匹配的研究

本文将动态描述逻辑与语义服务描述语言相结合,提出了一种动态语义描述方法来描述Web服务,并在经典服务匹配基础上进行改进,完善了服务匹配的类型,提高了Web服务的查全率与查准率。     

内质网应激对小鼠脂肪组织的影响

为了探究内质网应激对小鼠白色脂肪组织的影响,通过腹腔注射衣霉素(tunicamycin, TM)诱导小鼠体内内质网应激,构建内质网应激小鼠模型,然后获取小鼠附睾处白色脂肪组织,并采用RT-PCR和Western blot检测白色脂肪组织中内质网应激相关因子的mRNA水平及蛋白表达情况,通过荧光显微镜观察白色脂肪组织细胞的变化,测定甘油三酯、游离脂肪酸含量及脂肪细胞因子和炎症因子分泌情况.结果表明：1.0 mg/kg TM处理72 h诱导小鼠内质网应激效果最佳;内质网应激可以导致白色脂肪组织比例减少,脂肪细胞直径减小,脂解反应增多,脂肪细胞因子分泌减少及炎症反应增多等.该研究结果说明内质网应激可以诱导小鼠脂肪组织中发生脂解,进而介导脂代谢紊乱.     

发动机振动主动控制用电磁作动器的设计和动态特性研究

对一种适用于汽车发动机振动主动控制用的新型串接式高能电磁作动器进行了结构设计,计算了作动器的磁场,对作动器的片状弹簧的应力分布进行了有限元分析。同时,建立了作动器的力学模型,对其动态特性进行了仿真分析和试验研究,结果表明作动器是理想的激振源和主动控制用作动器。     

Angular glint calculation and analysis of radar targets via adaptive cross approximation algorithm

Angular glint is a significant electromagnetic （EM） scattering signature of extended radar targets. Based on the adaptive cross approximation （ACA） algorithm accelerated method of moments （MoM） and the plane incident wave assumption, the narrowband, wideband and newly developed high-resolution range profile （HRRP） based angular glint calculation formulations are derived and applied to arbitrarily shaped three-dimensional （3D） perfectly electrical y conducting （PEC） objects. In addition, the near-field angular glint is emphasized, which is of great importance for radarseeker applications. Furthermore, with the HRRP based angular glint, an approach to rigorously determine range resolution cel s which own relatively smal er angular glint is provided. Numerical results are presented with new findings to demonstrate the usefulness of the developed formulations.     

浅谈称重传感器工作原理及故障排除

称重传感器是电子计价秤的重要部件,它精度高、灵敏度高、稳定性强,具有抗偏载等多种性能,操作方便快捷可靠.它的作用是把加到秤盘上的物体重量转换成与该重量成比例的电信号.其工作原理是将输出的电信号经放大器放大,并经A/D 转换后由相关电路显示出称重信息.本文就称重传感器的应用进行了探讨.     

高等数学课程设置的多样化研究

由于专业和学生之间存在差异,在高等数学课程设置上必须实行多样化。具体说来,把高等数学的学习内容分为三个模块:基础模块、拓展模块(基地班)和应用模块,实践表明,这种多样化的高等数学课程设置取得了较好的教学效果。     

具有较小最大度的临界图边数的下界

运用Discharge法给出了不含2点和3点的6临界图及不含4点和5点的7临界图边数的下界．     

一种大管径煤气管道带压堵漏的应用

文章介绍了针对DN2200煤气总管泄漏,一种新型大管径煤气管道带压堵漏的性能和工作原理.     

国内高校人才培养模式发展趋势研究

从人才培养模式的定义出发,介绍了国内高校传统人才培养模式,总结了我国高校人才培养模式的发展趋势,认为不同的学校要根据自身的实际情况,结合社会需求构建科学的人才培养模式,形成自身的办学特色。