灵石县地膜覆盖旱地小麦适宜播期、播量的探讨

为了探讨灵石县旱地小麦地膜覆盖技术的配套措施,在2011年进行了相应的播期、播量的试验.结果表明,播期以9月24日-10月5日为宜.在该播期中,适宜的播量为10～18kg.从9月24日开始,播量为10kg,以后播期每向后推一天,播量需增加约0.75kg.     

响应面法优化提取腺叶桂樱叶的成分研究——总酚、总黄酮及DPPH自由基的清除活性

为了寻找新的天然抗氧化物,采用单因素试验、中心复合旋转设计和响应面法优化提取腺叶桂樱叶的总酚、总黄酮含量,并测试其DPPH自由基清除活性.结果表明最佳提取条件是60%乙醇,提取温度为48℃,提取时间为4 d; 该条件下,总酚含量、总黄酮含量分别为404.73 μg GAE/g DW,870.37 μg RE/g DW,DPPH的清除率为32.46%.该条件下试验值与预测值之间的相对误差低于5%,由此表明通过响应面法建立的二阶多项式模型可行.由此表明腺叶桂樱叶的乙醇提取物有较好的抗氧化活性.     

矮坨坨内生真菌Fusarium oxysporum代谢产物及其抗肝癌活性的研究

从矮坨坨根部分离得到的一株内生菌,经形态与分子生物学18S rDNA序列分析鉴定为尖孢镰刀菌(Fusarium oxysporum).运用中压柱色谱,高效制备液相色谱方法和现代波谱技术1HNMR、13CNMR以及LC-MS从其发酵液的乙酸乙酯部位分离鉴定了9个单体化合物,分别为4-氨基-2-羟基嘧啶(1),4,5-二氢-3H-吡唑-3-羧酸甲酯(2),4-羟基-7-(2-羟基-丁基)-3-甲基-氧杂环庚烷-2-酮(3),2-氨基-3-甲基戊酸(4),2,5-二氨基戊酸(5),2-氨基-3-甲基丁酸(6)4-羟基苯甲酸(7),脱氢镰刀菌酸(8),镰刀菌酸(9),其中化合物8对肝癌HepG2和Hep3B细胞具有较强的细胞毒活性,IC50值分别为23.9和29.1 μg/mL,而对正常肝细胞L-02未显示细胞毒性.     

双矩阵对策协同均衡的主对角占优准则及其算法

提出了判定双矩阵对策中协同均衡局势的"主对角占优"准则, 并从支付的角度对协同混合策略与混合策略以及协同均衡与纳什均衡的关系进行了探讨. 最后使用改进的PSO算法对协同均衡实现了近似求解.     

四苯基卟啉衍生物的拉曼光谱

介绍四苯基卟啉（TPP）及其中位取代氨基和硝基的拉曼光谱, 并讨论了给电子和吸电子取代基团对卟啉骨架振动的影响, 得出该取代对卟啉C_a—C_m和C_b—C_b的伸缩振动规律, 与四苯基卟啉相比, 引入给电子基团（氨基）使C_a—C_m向高波数方向移动, 吸电子（硝基）使C_a—C_m向低波数方向移动.      

基于人力资本视角下上海市高校体育教师培训体系的研究

运用人力资本理论,从改善上海市高校体育教师培训质量,优化体育教师人力资本等角度探讨了构建上海市高校体育教师培训体系的必要性,并设计出由培训需求评价、培训计划制定、培训过程实施与控制以及培训效果评估等各环节共同组成的科学培训体系。     

Genome-wide distribution of histone H3 acetylation in all- trans retinoic acid induced neuronal differentiation of SH-SY5Y cells

With chromatin immunoprecipitation (ChIP) and promoter DNA microarray analyses (ChIP-on-chip), we analyzed the variations of acetylation on histone H3 in all-trans retinoic acid (RA) induced neuronal cell differentiation. Neuroblastoma SH-SY5Y cells were treated with RA for 24 h and the acetylation on histone H3 in the promoter region of the genes was detected. Results showed that, after treatment, the level of acetylation on histone H3 elevated in 597 genes in the genome, and reduced in the other 647 genes compared with those of the control. In summary, we have successfully adopted a high throughput technique to detect and analyze variations of acetylation of histone H3 in human genome at the early phage of RA induced neuronal differentiation of the SH-SY5Y cells. Supported by National Natural Science Foundation of China (Grant Nos. 90408007 and 30721063) and National Key Basic Research and Development Program of China (Grant No. 2004CB518605)     

Evidence of vacuolar compartmentalization of arsenic in the hyperaccumulator Pteris vittata

Pteris vittata can hyperaccumulate arsenic （As） to 〉1% of its dry weight without showing any signs of toxicity, indicating the existence of effective plant-internal detoxificaUon mechanisms. Although vacuolar compartmentalization is known to play an important role in heavy metal detoxification and tolerance, direct evidence of arsenic compartmentalization in this hyperaccumulator was lacking. Here we report the subcellular localization of As in the callus of P. vittata. The callus induced from gametophytes of P. vittata exposed to 0.2 mmol/L arsenate for 20 days were examined by directly isolating cell walls, protoplasts and vacuoles, and determining arsenic concentrations. Of the total As in the callus, about 94% was in the protoplast, and of that, 91% was present in the vacuoles, indicating that vacuoles are a major storage site for As in P. vittata. In addition, the changes in the chemical components of vacuoles were analyzed by Fourier transform infrared spectroscopy （FTIR）.     

生态恢复与重建的新理论——生态发展

在总结了生态恢复与重建的现有理论和生态发展研究现状的基础之上,提出了生态恢复与重建的新理论——生态发展理论,构建了生态发展理论的理论基础,并对生态发展理论进行了诠释,阐述了生态发展理论对生态恢复与重建的重要意义．     

铜梁县玉米测土配方施肥试验及模型拟合研究

采用"3414"试验方案,以玉米"海禾1号"为材料,在铜梁县钙质石骨土上进行了氮、磷、钾肥料效应及模型拟合研究.结果表明:供试土壤中有效养分含量丰缺程度为N中等级、P高等级、K极高等级,14个处理中以N_2P_2K_2处理的产量最高,产量为5 583.0 kg/hm~2;用二次回归分析,得出氮磷钾的肥料效应函数方程为:y=3 103.31+8.32N+23.64P+16.90K-0.013N~2-0.10P~2-0.09K~2-0.011NP+0.01NK-0.16PK,玉米最高产量5 584.2 kg/hm~2时,N,P_2O_5,K_2O施用量分别为301.4,76.0,39.1 kg/hm~2,肥料施用比例为7.7:2:1.