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161.
María Camila Basto-Riascos Jhulyana López-Caro Fernando Vargas-Salinas 《Journal of Natural History》2017,51(41-42):2535-2550
Population and reproductive behaviour aspects of the glass frog Espadarana prosoblepon are well known for populations from Central America, but similar information is lacking for populations from South America. We recorded the reproductive ecology of a population of the glass frog E. prosoblepon in the city of Armenia, Central Andes of Colombia. With weekly surveys with mark-recapture between December 2013 and November 2015, we monitored activity patterns and evaluated if the probability of males mating is associated to their body size or to chorus tenure (i.e. the number of nights a same individual is calling for attracting a mate). In addition, upon observing an amplexus, we recorded the behaviour of the individuals until oviposition and noted characteristics of egg clutch. We recorded 47 males and 32 females, nine amplexus events, eight ovipositions, and 21 egg clutches (35.4 ± 4.79 eggs per clutch, N = 29). Activity of males and females and the number of egg clutches were positively correlated to rainfall. Mating success of males was not associated to their body size, but it was positively associated to longer chorus tenure; clutches from large females had a higher number of eggs than clutches from small females. Oviposition occurred on average 3.76 ± 1.74 hours after we first observed the amplexus, occurring 93.1% of the time in fronds of Selaginella geniculata at an average height of 1.58 ± 0.44 m. After the oviposition, the male left the site, while the female remained between 30 and 45 min, partially covering the eggs. The reproductive behaviour of E. prosoblepon did not vary widely between a population in Costa Rica and our population in time to oviposition, and mating success of males associated to chorus tenure; however, in our study population we recorded a larger clutch size and the preference for S. geniculata fronds as oviposition substrate. 相似文献
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Rike Wallbrecher Wouter P. R. Verdurmen Samuel Schmidt Petra H. Bovee-Geurts Felix Broecker Anika Reinhardt Toin H. van Kuppevelt Peter H. Seeberger Roland Brock 《Cellular and molecular life sciences : CMLS》2014,71(14):2717-2729
Binding to negatively charged heparan sulfates (HS) at the cell surface is considered the first step in the internalization of cationic cell-penetrating peptides (CPPs). However, little is known about the relation of the characteristics of the HS-CPP interaction such as affinity, stoichiometry, and clustering with uptake. In this study, we investigated a collection of mutants of a cyclic CPP derived from human lactoferrin with respect to HS binding and uptake. The thermodynamic parameters of HS binding were determined by isothermal titration calorimetry, clustering of HS was investigated by dynamic light scattering, and cellular uptake by flow cytometry and confocal microscopy. Whereas mutations of non-arginine amino acids that are conserved across lactoferrins of different mammalia only had a minor effect on uptake efficiency, changes in the number of arginine residues influenced the uptake significantly. In general, introduction of arginine residues and cyclization improved the HS affinity and the ability to cluster HS. In particular, there was a strong negative correlation between stoichiometry and uptake, indicating that crosslinking of HS is the driving force for the uptake of arginine-rich CPPs. Using glycan microarrays presenting a collection of synthetic HS, we show that a minimal chain length of HS is required for peptide binding. 相似文献
165.
Information about interpopulational variation of diet is important for thoroughly understanding the ecological niche of a species, the possible attributes that have allowed it to live in different habitats and ecosystems (if it is the case) and the degree of dietary specialization. This study compared dietary composition and diversity, size of prey consumed and feeding intensity between two geographically proximate populations of Craugastor alfredi, inhabiting contrasting habitats: one located in a remnant tropical rainforest and the other population located within a cave entrance, in eastern Mexico. A total of 118 frogs (50 from rainforest and 68 from cave) were stomach-flushed and, from those, 86 had at least one prey item (42 from rainforest and 44 from cave). Consumption proportions of orthopterans differed between habitats, with frogs from the cave having higher importance values of this prey than frogs from the rainforest. Additionally, both dietary diversity and feeding intensity were higher in the rainforest population. It was concluded that the generalized diet of C. alfredi, besides the high consumption of orthopterans by the cave population, may have contributed to its success in cave entrances. Nonetheless, other non-feeding attributes like small body size, direct development and semiarboreality may also facilitate the use of rock crevices in caves for foraging and reproduction. 相似文献
166.
Vincent A. van der Mark Mohammed Ghiboub Casper Marsman Jing Zhao Remco van Dijk Johan K. Hiralall Kam S. Ho-Mok Zoë Castricum Wouter J. de Jonge Ronald P. J. Oude Elferink Coen C. Paulusma 《Cellular and molecular life sciences : CMLS》2017,74(4):715-730
P4-ATPases are lipid flippases that catalyze the transport of phospholipids to create membrane phospholipid asymmetry and to initiate the biogenesis of transport vesicles. Here we show, for the first time, that lipid flippases are essential to dampen the inflammatory response and to mediate the endotoxin-induced endocytic retrieval of Toll-like receptor 4 (TLR4) in human macrophages. Depletion of CDC50A, the β-subunit that is crucial for the activity of multiple P4-ATPases, resulted in endotoxin-induced hypersecretion of proinflammatory cytokines, enhanced MAP kinase signaling and constitutive NF-κB activation. In addition, CDC50A-depleted THP-1 macrophages displayed reduced tolerance to endotoxin. Moreover, endotoxin-induced internalization of TLR4 was strongly reduced and coincided with impaired endosomal MyD88-independent signaling. The phenotype of CDC50A-depleted cells was also induced by separate knockdown of two P4-ATPases, namely ATP8B1 and ATP11A. We conclude that lipid flippases are novel elements of the innate immune response that are essential to attenuate the inflammatory response, possibly by mediating endotoxin-induced internalization of TLR4. 相似文献
167.
A polycaprolactone-grafted cellulose nanocrystal(PCL-g-CN) was prepared in ionic liquid and characterized by Fourier transform infrared spectrum(FT-IR),X-ray diffraction(XRD),and transmission electron microscopy(TEM).A peak assigned to the carbonyl group appears at 1 730 cm~(-1) in the FT-IR of PCL-g-CN,which confirms that the grafting reaction is successfully completed.The morphologies of PCL-g-CNs still maintain rod-like structure according to the TEM images.XRD results show that the crystal type of the PCL-g-CNs changed from cellulose Ⅰ to cellulose Ⅱ.The reasons for crystal transition of CNs turn out to be combined effects of anion and cation in ionic liquid with CNs. 相似文献
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169.
Paloma Feyjoo Miguel Cabanellas-Reboredo Matías Calvo-Manazza Beatriz Morales-Nín Jorge Hernández-Urcera Manuel E. Garci 《Journal of Natural History》2016,50(9-10):543-555
The embryonic development of the squid Loligo vulgaris was observed from 183 egg masses collected from special devices deployed throughout Cabrera National Park (Baleares Islands, western Mediterranean Sea). Sequence alignment analysis of the cytochrome oxidase I gene revealed that all embryos belonged to L. vulgaris. In total, 549 egg capsules were examined. Viable egg capsules (n = 420) were classified into one of five maturation stages according to the primary external features. The length of the viable egg capsules varied between 40 and 170 mm, and increased with embryonic development. The non-viable capsules (n = 129) were categorized into four groups: I (Ginger root), non-viable II and III, and empty egg capsule (IV). The percentage of non-viable capsules (i.e. grades I, II and III) was 92.25%. Empty capsules accounted for 7.75% of the total non-viable egg capsules. Embryonic development was classified into a second scale of eight stages. Egg capsule stage and embryonic stage were significantly related (n = 420; p < 0.001), facilitating the determination of the embryo developmental phase based on the outward appearance of the egg capsules. The embryo development stage based on the external features of the egg capsules might constitute an innovative tool for in situ embryological data collection. This new method is neither time consuming nor invasive, and could be helpful in fishing cruises, for scuba diving visual census in natural habitats and for laboratory culture. Slight variability in the developmental embryonic stages within egg capsules from the same egg mass was identified. The origin of this asynchrony is discussed. Chronological appearance of organs was similar to that of the six loliginid species previously examined. However, some developmental changes in the timing or rate of events (heterochronies) were observed: Hoyle’s organ was formed earlier in L. vulgaris and the appearance of ventral chromatophores was slightly delayed (2 days) compared with the other species considered. 相似文献
170.
Dealing with Distances and Transformations for Fuzzy C-Means Clustering of Compositional Data 总被引:1,自引:0,他引:1
Javier Palarea-Albaladejo Josep Antoni Martín-Fernández Jesús A. Soto 《Journal of Classification》2012,29(2):144-169
Clustering techniques are based upon a dissimilarity or distance measure between objects and clusters. This paper focuses on the simplex space, whose elements??compositions??are subject to non-negativity and constant-sum constraints. Any data analysis involving compositions should fulfill two main principles: scale invariance and subcompositional coherence. Among fuzzy clustering methods, the FCM algorithm is broadly applied in a variety of fields, but it is not well-behaved when dealing with compositions. Here, the adequacy of different dissimilarities in the simplex, together with the behavior of the common log-ratio transformations, is discussed in the basis of compositional principles. As a result, a well-founded strategy for FCM clustering of compositions is suggested. Theoretical findings are accompanied by numerical evidence, and a detailed account of our proposal is provided. Finally, a case study is illustrated using a nutritional data set known in the clustering literature. 相似文献