全文获取类型
收费全文 | 967篇 |
免费 | 3篇 |
国内免费 | 3篇 |
专业分类
系统科学 | 7篇 |
丛书文集 | 1篇 |
教育与普及 | 3篇 |
理论与方法论 | 14篇 |
现状及发展 | 83篇 |
研究方法 | 183篇 |
综合类 | 619篇 |
自然研究 | 63篇 |
出版年
2021年 | 1篇 |
2020年 | 4篇 |
2018年 | 6篇 |
2017年 | 13篇 |
2016年 | 7篇 |
2015年 | 5篇 |
2014年 | 7篇 |
2013年 | 9篇 |
2012年 | 84篇 |
2011年 | 156篇 |
2010年 | 23篇 |
2009年 | 9篇 |
2008年 | 76篇 |
2007年 | 82篇 |
2006年 | 91篇 |
2005年 | 86篇 |
2004年 | 67篇 |
2003年 | 75篇 |
2002年 | 89篇 |
2001年 | 2篇 |
2000年 | 19篇 |
1999年 | 4篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1970年 | 5篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有973条查询结果,搜索用时 0 毫秒
901.
902.
Functional profiling of the Saccharomyces cerevisiae genome 总被引:1,自引:0,他引:1
Giaever G Chu AM Ni L Connelly C Riles L Véronneau S Dow S Lucau-Danila A Anderson K André B Arkin AP Astromoff A El-Bakkoury M Bangham R Benito R Brachat S Campanaro S Curtiss M Davis K Deutschbauer A Entian KD Flaherty P Foury F Garfinkel DJ Gerstein M Gotte D Güldener U Hegemann JH Hempel S Herman Z Jaramillo DF Kelly DE Kelly SL Kötter P LaBonte D Lamb DC Lan N Liang H Liao H Liu L Luo C Lussier M Mao R Menard P Ooi SL Revuelta JL Roberts CJ Rose M Ross-Macdonald P Scherens B Schimmack G 《Nature》2002,418(6896):387-391
Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth under six well-studied conditions: high salt, sorbitol, galactose, pH 8, minimal medium and nystatin treatment. Less than 7% of genes that exhibit a significant increase in messenger RNA expression are also required for optimal growth in four of the tested conditions. Our results validate the yeast gene-deletion collection as a valuable resource for functional genomics. 相似文献
903.
904.
905.
906.
Annexin V was originally identified as a collagen-binding protein called anchorin CII and was isolated from chondrocyte membranes
by affinity chromatography on native type II collagen. The binding of annexin V to native collagen type II is stable at physiological
ionic strength when annexin V is reconstituted in liposomes. The binding to native collagen types II and X, and to some extent
to type I as well, was confirmed using recombinant annexin V. A physiological role for annexin V interactions with extracellular
collagen is consistent with the localization of annexin V on the outer cell surface of chondrocytes, microvilli of hypertrophic
chondrocytes, fibroblasts and osteoblasts. A breakthrough in our understanding of the function of annexin V was made with
the discovery of its calcium channel activity. At least one of several putative functions of annexin V became obvious from
studies on matrix vesicles derived from calcifying cartilage. It was found that calcium uptake by matrix vesicles depend on
collagen type II and type X binding to annexin V in the vesicles and was lost when collagens were digested with collagenase;
calcium influx was reconstituted after adding back native collagen II or V. These findings indicate that annexin V plays a
major role in matrix vesicle-initiated cartilage calcification as a collagen-regulated calcium channel. 相似文献
907.
908.
Mark R. Roest 《武汉大学学报:自然科学英文版》1996,1(3-4):410-419
In spite of the wealth of existing data distribution methods, most parallel programming languages support only some form of cyclic blockwise distribution. The main reason why only this single method is supported is that it is relatively simple to implement. However, it is as yet unclear whether cyclic blockwise distribution is sufficiently powerful for a wide class of distribution problems. In this paper the method will be analysed, showing that for a wide range of problems it is indeed sufficient. It will also be shown in which cases cyclic blockwise distribution can be expected to fail. From this analysis, it is possible to formulate practical guidelines to assist programmers in choosing the cycle frequency for cyclic blockwise distribution that leads to an optimal result. 相似文献
909.
910.