砼裂纹分析及防治

砼工程中出现裂纹是典型的质量通病，砼裂纹不仅减少砼结构的使用寿命，而且给人们一种观外上的危险感，实践证明，只要严格按施工规范施工，抓好每一道工序质量，完全可以避免砼裂纹的产生，论述了砼裂纹产生的原因和机理，提出了有效的防治措施。     

Epigenome analyses using BAC microarrays identify evolutionary conservation of tissue-specific methylation of SHANK3

CpG islands are present in one-half of all human and mouse genes and typically overlap with promoters or exons. We developed a method for high-resolution analysis of the methylation status of CpG islands genome-wide, using arrays of BAC clones and the methylation-sensitive restriction enzyme NotI. Here we demonstrate the accuracy and specificity of the method. By computationally mapping all NotI sites, methylation events can be defined with single-nucleotide precision throughout the genome. We also demonstrate the unique expandability of the array method using a different methylation-sensitive restriction enzyme, BssHII. We identified and validated new CpG island loci that are methylated in a tissue-specific manner in normal human tissues. The methylation status of the CpG islands is associated with gene expression for several genes, including SHANK3, which encodes a structural protein in neuronal postsynaptic densities. Defects in SHANK3 seem to underlie human 22q13 deletion syndrome. Furthermore, these patterns for SHANK3 are conserved in mice and rats.     

中文自动标引的现状分析和展望

文章论述了国中文自动标引这一重要研究方向的现状，出现的一些比较曲型的标引方法，提出了中文自动标引的难点，相关技术和今后进一步发展的方向。     

带时变时滞的不确定离散奇异系统鲁棒稳定性分析

为了降低不确定离散奇异时变时滞系统稳定性条件的保守性,首先,采用时滞分割方法,获得了新的时滞系统描述方法,并通过综合考虑各时滞分割子区间,提出了分割子区间依赖型Lyapunov函数.其次,采用时滞依赖线性矩阵不等式技术,将研究结果描述成易于求解的严格线性矩阵不等式形式.通过Matlab工具箱求解线性矩阵不等式,即可获得标称系统正则、因果及均方稳定的条件,并将标称系统的研究结果推广至不确定离散奇异时变时滞系统的稳定性分析,获得了不确定离散奇异时变时滞系统鲁棒稳定判定条件.最后通过实例应用说明了所提定理的有效性,且与现有文献结果相比,保守性得到了较大程度的降低.     

ZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner

R-spondin proteins strongly potentiate Wnt signalling and function as stem-cell growth factors. Despite the biological and therapeutic significance, the molecular mechanism of R-spondin action remains unclear. Here we show that the cell-surface transmembrane E3 ubiquitin ligase zinc and ring finger 3 (ZNRF3) and its homologue ring finger 43 (RNF43) are negative feedback regulators of Wnt signalling. ZNRF3 is associated with the Wnt receptor complex, and inhibits Wnt signalling by promoting the turnover of frizzled and LRP6. Inhibition of ZNRF3 enhances Wnt/β-catenin signalling and disrupts Wnt/planar cell polarity signalling in vivo. Notably, R-spondin mimics ZNRF3 inhibition by increasing the membrane level of Wnt receptors. Mechanistically, R-spondin interacts with the extracellular domain of ZNRF3 and induces the association between ZNRF3 and LGR4, which results in membrane clearance of ZNRF3. These data suggest that R-spondin enhances Wnt signalling by inhibiting ZNRF3. Our study provides new mechanistic insights into the regulation of Wnt receptor turnover, and reveals ZNRF3 as a tractable target for therapeutic exploration.     

Re-emerging superconductivity at 48 kelvin in iron chalcogenides

Pressure has an essential role in the production and control of superconductivity in iron-based superconductors. Substitution of a large cation by a smaller rare-earth ion to simulate the pressure effect has raised the superconducting transition temperature T(c) to a record high of 55?K in these materials. In the same way as T(c) exhibits a bell-shaped curve of dependence on chemical doping, pressure-tuned T(c) typically drops monotonically after passing the optimal pressure. Here we report that in the superconducting iron chalcogenides, a second superconducting phase suddenly re-emerges above 11.5?GPa, after the T(c) drops from the first maximum of 32?K at 1?GPa. The T(c) of the re-emerging superconducting phase is considerably higher than the first maximum, reaching 48.0-48.7?K for Tl(0.6)Rb(0.4)Fe(1.67)Se(2), K(0.8)Fe(1.7)Se(2) and K(0.8)Fe(1.78)Se(2).     

中国剩余定理及其应用——基于研究性学习的设计

中国剩余定理在数论及代数理论的研究中起着重要的作用,是一个极其重要的定理.通过中国剩余定理的历史起源来给出该定理及其证明方法,在此基础上对该定理的应用进行了讨论和分析,并给出了一些例子.     

基于分形布朗业务模型的差分队列服务建模

差分队列服务(DQS)是一种在有线与无线融合网络中能有效提供服务质量的服务质量模型,文中基于DQS的思想,将具有相同时延要求的到达业务作为一个分形布朗运动(FBM)业务,建立了一个针对多个FBM业务及对应时延要求的差分队列服务模型,并推导出该模型下的丢包率公式.仿真结果表明,文中模型分析的结果与基于数据包粒度的仿真结果是一致的.模型性能分析结果表明,实时业务的突发性对差分队列服务的服务质量保证影响较大.     

Preparation and cellular uptake of PLGA particles loaded with lamivudine

Poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles loaded with lamivudine and coated with bovine serum albumin (BSA) were prepared via a double emulsion method. The influences of experiments parameters such as volume of inner aqueous phase, concentration of organic phase and ultrasonication time on the particle size and drug entrapment efficiency were investigated, obtaining PLGA particles with a diameter of ~260 nm and drug entrapment efficiency of ~35%. The particles were observed by scanning electron microscopy and transmittance electron microscopy, showing a core-shell structure. BCA assay found that 58 mg BSA was present on/in 1 g LPB particles. The loaded lamivudine showed a burst release at beginning and sustained release until 24 h in physiological conditions. Low pH could accelerate the release of lamivudine from PLGA particles, making the PLGA particles potential intelligent intracellular drug carriers. The PLGA particles were readily internalized into the human liver cells within a short time and increased gradually with the prolongation of incubation time regardless of the loading of lamivudine. The particles either resided within lysosomes or transferred to cytoplasm, but could not enter into the cell nucleus. The cell viability was not significantly influenced in the presence of the particles regardless of lamivudine encapsulation, suggesting that this kind of particles may be a good candidate for the intracellular anti-hepatitis B drug delivery.     

基于云平台的软件服务流引擎技术研究

传统的工作流自动化处理大多是针对固定的较为简单的执行单元,不能适应复杂的SaaS服务要求。为同时向大规模访问用户提供可靠实时的云计算服务,文章设计了软件服务流,高效、实时、稳定的调度算法是软件服务流成功实施的关键。通过服务抽象过程把复杂的用户自定制请求匹配成云平台的可执行单元后,软件服务流引擎利用文中提出的EOE实时调度算法处理用户请求,交付下层执行,监控执行进程、系统状态,实现软件服务流的自动化高效运行。最后,给出了在海洋环境信息云服务体系框架下的实现。