重组抑肽酶的表达纯化和活性测定

抑肽酶是一种用途广泛的丝氨酸蛋白酶抑制剂.用基因重组技术将化学合成的抑肽酶基因插入表达载体pET28a,并转化至大肠杆菌Rosetta(DE3)中,经IPTG诱导后,表达产物以包涵体形式存在于菌体内包涵体纯化后经复性及CM-阳离子交换层析后,从每升培养液可获得2.4 mg抑肽酶,纯度可达90%.活性测定结果显示,重组抑肽酶可竞争性抑制纤溶酶对小分子底物S2251的水解活性.抑制常数(Ki)为(0.6±0.3)nmol/L,与天然抑肽酶的抑制活性相似.     

输水管道穿南水北调总干渠方案研究

根据南水北调总干渠总体设计技术规定:输水管道穿越总干渠,其穿越建筑物不占用总干渠水头.为满足总体设计要求,根据输水管道管底高程、总干渠渠底高程及设计水位的相互关系,考虑了渠上管道桥和渠底倒虹吸两种穿越方案,通过技术、经济、施工等综合分析比较,确定输水管道穿总干渠建筑物型式为渠底倒虹吸,倒虹吸管外加防护箱涵.     

Motion feature descriptor based moving objects segmentation

A novel moving objects segmentation method is proposed in this paper.A modified three dimensional recursive search (3DRS) algorithm is used in order to obtain motion information accurately.A motion fea...     

应用改进的灰关联法对供应链绩效评价的研究

随着全球经济的发展和全球制造的出现,供应链越来越受到了人们的关注,并在各个行业,特别是制造业中得到了普遍应用,成为一种新的管理模式。在供应链管理体系中,为了确保供应链管理能够健康、可持续地发展,建立科学、全面的供应链绩效评价体系,已经成为一个迫切需要解决的问题。文章应用主成分分析法与问卷调查法对绩效评价的指标进行降维与筛选,运用改进的灰关联法对供应链绩效进行整体评价。     

第二代居民身份证防伪特征的研究

第二代居民身份证的制作过程中应用了多种防伪技术,其中包括数字防伪技术、射频识别技术和印刷防伪技术,使得制作出的身份证具有不同的防伪特征。印刷防伪技术包括:版纹防伪技术(接线印刷技术和缩微文字印刷技术)、紫外荧光油墨印刷技术、光变油墨印刷技术和微透镜微图形组合薄膜技术。     

城市旅游发展动力系统视角下的旅游产品开发——以南京市为例

以南京市为例构建城市旅游发展驱动力模型判别指标体系,采用AHP法和多目标线性加权函数法得到南京市旅游发展驱动力得分及结构并与理想旅游城市比较分析,结果表明南京市旅游发展存在旅游产品结构单一、供需脱节、品牌形象缺失等问题.然后提出相应升级方向:塑造鲜明的品牌形象,打造高端旅游产品,挖掘特色产品,添加参与性、体验性要素,引入另类旅游产品,培育生态旅游产品,并注重产品整合和规模效益、集聚效益的发挥.本研究首次将客源地旅游需求系统纳入城巾旅游发展驱动力指标体系,利用定量方法分析了南京城市旅游产品存在的问题并提出相应对策,为发达地区面临竞争的旅游城市产品开发研究提供了借鉴和指导.     

Low concentrations of NaHSO3 enhance NAD(P)H dehydrogenase-dependent cyclic photophosphorylation and alleviate the oxidative damage to improve photosynthesis in tobacco

Bisulfite at low concentrations(L-NaHSO3) increases cyclic electron transport around photosystem I(PSI) and photosynthesis.However,little is known regarding the detailed contribution of cyclic electron transport to the promoted photosynthesis by L-NaHSO3.In the present work,we used tobacco mutant defective in ndhC-ndhK-ndhJ(ndhCKJ) to investigate the role of NAD(P)H dehydrogenase(NDH)-dependent cyclic electron transport around PSI in an increase in photosynthesis by L-NaHSO3.After the treatment of tobacco leaves with L-NaHSO3(10 μmol L-1),the NDH-dependent cyclic electron transport,monitored by a transient post-illumination increase in Chl fluorescence and the amount of NDH,was notably up-regulated in wild type(WT).The NDH-dependent cyclic electron transport was severely impaired in ndhCKJ and was not significantly affected by treatment with L-NaHSO3.Accordingly,the NDH-dependent transthylakoid membrane proton gradient(pH),as reflected by the slow phase of millisecond-delayed light emission(ms-DLE),was increased by L-NaHSO3 in WT,but not in ndhCKJ;the enhancement of cyclic photophosphorylation(PSP) activity by L-NaHSO3 was more obvious in WT than ndhCKJ.The accumulation of both superoxide and hydrogen peroxide was reduced in WT when subjected to L-NaHSO3 treatment,but not in ndhCKJ.Furthermore,the increase of photosynthetic O 2 evolution rate by L-NaHSO3 was more significant in WT than in ndhCKJ.We therefore conclude that L-NaHSO3 alleviates the photo-oxidative damage by the enhancement of NDH-dependent cyclic PSP,thereby improving photosynthesis.     

Morphology of polar ionospheric O^＋ ion upflow： FAST observations during quiet time

With energetic ion measurements on FAST satellite, the morphologic features of quiet-time ionospheric O ion upflowing at altitudes of 2000 to 4000 km are drawn out for the first time. The pre-noon cusp/cleft is the predominant region of upflowing occurrence for O with lower energy. Meanwhile the pre-dawn sector near the equatorward edge of the plasma convection dominates the occurrence for the higher energy O ions. No matter whether the energy is lower or higher, the upflows occur often over a wide MLT range of lower latitudes outside the auroral oval. The upflowing within the pre-midnight (21:00― 22:00 MLT) auroral oval carrys larger energy fluxes, with extremely large fluxes for higher energy O appearing near the polar cap boundary. For altitudes of 2000―4200 km under observation, the ion conics occur much more frequently than ion beams. Ion beams are rarely found below 3000 km, while the conics occur uniformly over the observed altitudes.     

原子核液-气相变的实验观察

1IntroductionPhase transition and critical phenomenon are extensively debatable subjects in the natural sciences.Re-cently,the same concept was introduced into the astronomical objects[1]as well as the microscopic systems,such as in atomic cluster[2]and n…     

羌族非物质文化遗产的现状及保护对策——以阿坝藏族羌族自治州为例

以羌族的主要聚居区——阿坝藏族羌族自治州为例,对羌族的非物质文化遗产的现状加以梳理,认为羌族非物质文化遗产的保护要积极发挥阿坝州的先天优势,依托州内高校和博物馆的科研力量,发动羌族学者和广大民众来实现,同时避免商业化炒作和借开发为名的人为破坏。