Expression and Characterization of HGV E2 cDNA in Pichia pastoris

A 559 base pair fragment of cDNA locating at the putative E2 region of GBV-C/HGV was in-serted into Pichia pastoris expression vector pPICgK in the reading frame of α-factor secreting signal pep-tide. The recombinant expression plasmid pPIC9K-E2 was introduced into P. pastoris GSll5 with electro-poration and recombined with the host genome by homological recombination. The His+Mut+ recombinantyeasts were selected and cultivated in the BMMY medium. After 3 days induction with 0. 5% methanol,the target protein(E2) accumulated up to 30% of total proteins in the supernatant. The expressed E2 pro-tein was proved possessing antigenicity and high specificity with Western blot and ELISA probed with serafrom the immunized rabbits and the patients infected by GBV-C/HGV.     

AC发泡剂新工艺研究—Ⅲ电解氧化法

用钌钛金属阳极在含有Ｃｌ—和Ｂｒ－离子的酸性溶液中进行电解氧化联二脲制备偶氮二甲酰胺，获得产品得率为９３—９８％、纯度为９９．６％、电流效率为８８－９２％。母液可循环使用，基本不产生废液．     

150m跨径PC矮塔斜拉桥施工技术介绍

山西太原市西北环高速公路汾河特大桥主桥采用预应力钢筋混凝土矮塔斜拉桥结构形式,本文主要介绍了大桥的施工概况和主要工序的技术、措施。     

关于提升企业核心力的思考

指出企业提升竞争力的重要战略是建立自身特色的企业文化,企业文化已成为企业竞争的核心元素;企业竞争不仅需要企业有过硬的工程质量,更需要具有深厚的文化内涵工程来支撑;重视企业文化与否,决定着企业在激烈的市场竞争中的成功与失败.论述了企业创新发菜的需要、企业主导作用的发挥、企业文化氛围的营造,企业文化模式的确立、企业形象的塑造.     

加强文献资源建设为教学科研服务

文章从文献资源建设的作用和重要性出发,介绍了文献建设的原则与方法,以及做好文献资源建设工作应正确处理好的几种关系,提出了文献资源建设要通过藏书剔旧工作来保持稳定的建议,论述了文献资源建设为教学科研服务的新策略和馆藏政策制定方式。     

工程项目风险评价方法的研究

工程项目风险评价方法的研究傅鸿源（重庆建筑大学管理学院，６３００４５）ＡＭｅｔｈｏｄｏｌｏｇｙｆｏｒＥｖａｌｕａｔｉｎｇＰｒｉｊｅｃｔ’ｓＲｉｓｋＦｕＨｏｎｇｙｕａｎ（ＣｈｏｎｇｑｉｎｇＪｉａｎｚｈｕＵｎｉｖｅｒｓｉｔｙ，６３００４５）Ａｂｓｔｒａｃ...     

森林面积分布系统的最优控制存在性问题

本文研究森林面积分布系统的最优控制问题。本文将林木总和长消率作为控制变量，以“范数最小”来衡量其最优性，利用空间Ｌ＾２（０，Ｔ）Ｔ〉０的自反、光滑和严格凸性，给出了森林面积分布系统最优控制的一个存在性定理。     

多信息水声传输方式及其应用研究

介绍“抗强噪声水层深度遥测装置”专利中,采用频分PPM(Pulse-Position modulation)方式进行多信息水声传输的研究和工程设计,并着重阐述产生PPM数字信号的新方法和应用特殊的数字选频滤波接收的先进技术,并提出所设计的有关电路。     

可压缩流体网络的建模方法

本文运用网络分析手段,首次提出了任意拓扑结构可压缩流体网络动态特性的建模方法.该方法突破了目前在流体网络分析中普遍采用的“小扰动”线性化处理方法的局限。     

Learning attention-based strategies to cooperate for multi-agent path finding

Multi-agent path finding (MAPF) is a challenging multi-agent systems problem where all agents are required to effectively reach their goals concurrently with not colliding with each other and avoiding obstacles. In MAPF, it is a challenge to effectively express the observation of agents, utilize historical information, and effectively communicate with neighbor agents. To tackle these issues, in this work, we proposed a well-designed model that utilizes the local states of nearby agents and outputs an optimal action for each agent to execute. We build the local observation encoder by using residual attention CNN to extract local observations and use the Transformer architecture to build an interaction layer to combine local observations of agents. With the purpose of overcoming the deficiency of success rate, we also designed a new evaluation index, namely extra time rate (ETR). The experimental results show that our model is superior to most previous models in terms of success rate and ETR. In addition, we also completed the ablation study on the model, and the effectiveness of each component of the model was proved.