Effects of maternal aging on localizations and expression levels of DNA methyltransferases and chromosome architecture in in-vivo matured mouse oocytes

This paper investigated the age-related changes in the expression patterns of maintenance methyltransferase (DNMT1) and de novo methyltransferases (DNMT3a, 3b, 3L) and the chromosome architecture in in-vivo matured mouse oocytes using two-photon laser-scanning microscope. Our results showed that (1) DNMT1 and DNMT3a, 3b, 3L in the oocytes of pubertal mice were located in the cortical region of oocyte cytoplasm. In aging groups, DNMT1 was also located in the cortical region. However, DNMT3a, 3b, 3L had a relatively wider distribution in the oocyte cytoplasm and appeared near the chromosomes. These differences between pubertal and aging groups suggested that aging might affect DNA methylation; (2) the expression of DNMT1, and DNMT3a, 3b in aging groups increased significantly compared to pubertal groups, while, the expression of DNMT3L decreased. These results might be explained by the compensation mechanism among DNMTs, which might be impervious to aging; (3) aging caused increased errors in the distribution and three-dimensional morphology of chromosomes, including the increased total volume and surface area, the high ratio of height to diameter of a circular cylinder enclosing the chromosomes (H/D). Our work provided morphological information for the studies of age-related decline in oocyte qualities.     

法语在法国对外文化政策的地位及其在中国的表现

法语的推广在法国对外文化推广中的作用是非常重要的.文章将对法国对外文化政策的起源、社会背景、法语的重要地位以及结合中国实际进行了综合分析研究.     

大学自治的演变及其有限性

大学自治是大学的精神,但自治却随着历史的发展而逐渐改变。当然,从来没有绝对的大学自治,一所大学的存在受到诸多因素的影响。无论怎样,大学应该有自身的立场和原则,不能轻易地因其他因素的影响而改变,这是一所真正的大学之所以生存和发展的关键因素。     

面具下的荒诞——《路的尽头》之真相

约翰·巴思是当代美国最有影响力的后现代主义小说家之一,他的第二部作品《路的尽头》充分讨论了＂面具＂的深刻含义。人格面具是一把双刃剑：它是人适应社会的重要条件,但在整个人格中也会产生负面影响。运用面具理论,分析小说中主人公雅各布·霍纳错综复杂的思想,探讨人格面具对他生活所造成的影响;分析导致霍纳人格面具膨胀和人格分裂的深层社会原因。可以发现,巴思正是试图通过借助霍纳所戴的面具,嘲讽当时虚无、荒诞和存在焦虑的社会思潮。     

人民调解的体系重构——以云南省沾益县“一十百”为例

人民调解在我国基层社会的治理中一直具有不可替代的地位和作用,但其体系一直是混乱和不系统的.云南省沾益县“一十百”管理模式从纠纷解决的角度,对人民调解体系进行重构.强调以十户代表、百户代表为主体架构,以个人调解工作室为合理补充,以司法确认为最终保障的合理体系,并构建和完善其评价标准,以保证这一体系的健康发展.     

Chiral pyrrolidine-azole conjugates: Simple and efficient asymmetric organocatalysts for Michael addition to nitrostyrenes

Simple pyrrolidine-azole conjugates have been synthesized and found to be efficient catalysts for asymmetric Michael addition to nitrostyrenes. The identified optimal catalysts, pyrrolidine-azoles 2, 8 and 13, could catalyze the asymmetric Michael addition of a range of Michael donors and nitrostyrenes in high yields (up to 99%) and excellent stereoselectivities (up to 99:1 dr and 97% ee).     

K148与K149对黑曲霉NRRL3135植酸酶A与植酸结合机制的影响

为了研究黑曲霉NRRL3135植酸酶A K148与K149对底物与酶的结合反应的影响,构建了一个单位点突变体(K148E)和一个双位点突变体(K148E和K149E),由于带电的氨基酸残基发生变化,造成这2个突变体的酶活性都有不同程度的减少,利用InsightII软件模拟了2种突变体酶的3-D结构,发现酶分子突变位置的表面电势有明显的改变.这2个α-螺旋(141～160)位点的改变可能降低了活性中心附近的底物浓度,减缓了底物与活性中心的反应速度.     

Sequence similarity analysis of non-self CTL epitopes and mouse proteins using sequence alignment

This research analyzed amino acid sequence similarity between non-self T cell epitopes recognized by mouse antibodies and mouse proteins. Using sequence alignment,we found that only 8 of 1 108 epitopes are highly similar to mouse protein sequences. The result shows that non-self T cell epitopes are not similar or have little similarity to mouse protein sequences. Furthermore,reviewing the related literature,we also found that the eight epitopes would trigger immune responses in some particular environment,which are ignored by T cells in normal condition. The result suggests that no or low-similarity peptide vaccines can reduce the chance of collateral cross-reactions and enhance the antigen-specific immune response to vaccine.     

低空数码摄影测量的应用探讨

介绍了利用低空数码航测法生产3D产品的技术流程,以河南省新密市测区为例,从低空航测必备条件、技术参数等方面探讨了低空数码摄影测量生产3D产品的组织实施,并指出了目前航测法存在的不足之处。     

师生谈心高效之浅见

在平时的教学工作中,师生交流与沟通只要能重视方式方法,就能增进师生间相互理解、支持与配合,建立浓厚的师生感情,产生凝聚力、向心力,有利于良好学风的形成,促进学生身心健康发展.那么,教师应如何与学生谈心才能更高效呢.笔者对此进行了论述.