A comparison between the photoreactivity of some furocoumarins with native DNA and their skin-photosensitizing activity

Riassunto È stata determinata la fotoreattività con DNA nativo di un gruppo di furocumarine per irradiazione a 365 nm. I valori ottenuti sono risultati in accordo con quelli di attività fotosensibilizzatrice cutanea posseduti dalle stesse sostanze.     

A simple culture vessel for turbidimetric studies of anaerobic growth of microbes

Résumé La fiole de culture décrite permet le mesurage par voie turbidimétrique de la croissance microbienne en culture anaérobie et agitée. Du gaz, produit par la culture, peut s'échapper par une valve. Par l'application additionelle d'un bulbe latéral contenant de la potasse, la comparaison est possible entre des cultures parallèles, croissant soit en présence soit en absence de CO₂.

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The first author is indebted to Prof. Dr.T. O. Wikén for facilities offered to him in the Laboratory of Microbiology, Technological University, Delft.     

Theory agrees with experimental thermal denaturation of short DNA restriction fragments

Experimental melting transitions of several natural DNAs of known nucleotide sequences have recently been obtained. The differential melting curves of these DNAs-phi X174 DNA, fd DNA and SV40 DNA-all show distinctive sets of peaks or fine structure. Theoretical melting curves calculated from the sequences and a few a priori parameters have not accurately predicted the experimental transitions. Although calculated fine structure resembled experimental curves in some cases, the characteristic features of a DNA's differential melting curve could not generally be produced. Azbel and Gabbarro-Arpa et al. have recently obtained good agreement between calculated and experimental curves using a different theoretical approach-only ground-state configurations of DNA were considered for temperatures inside the transition region. Their results suggest that the basic model of DNA melting, common to all theoretical approaches, is accurate. We have used here an exact theoretical approach to calculated melting curves of four DNA restriction fragments of 95-301 base pairs containing the lactose promoter region (Fig. 1). Theoretical curves agree very well with the experimental transitions published by Hardies et al. and obtained in this laboratory.     

Cytochemically rich neuroglia invest metacercariae in the brain ofPhoxinus phoxinus L.

Summary Histochemical determination of neuroglia investing parasites in naturally infected fish depends upon recognition of the highly soluble or diffusible nature of the cytoplasm.Acknowledgment. M.R.L. Johnston advised me during the course of this work.