Decrease of mast cells in regional lymph nodes in response to allogeneic antigens and syngeneic tumor antigens

Summary The absolute number of mast cells in regional lymph nodes decreases on the 5th day after stimulation by allogeneic lymphocytes and semisyngeneic leukaemic cells, despite the enlargement of stimulated lymph nodes. It is postulated that the reaction of lymphatic mast cells could be a sensitive test for tissue incompatibility, and probably also for the presence of tumor associated antigens.     

Orientation of Giemsa C-bands in interphase cells of Allium cepa L.

Summary Orientation of Giemsa C-bands in Allium cepa was studied in both mitotic and interphase cells. It has been shown that telophase orientation of the chromosome is maintained throughout the interphase and early prophase. It has been assumed that this non-random orientation is due to anchorage of the telomeres with the nuclear membrane. Contrary to earlier observations, 2 by 2 pairing of the telomeres could not be traced in this species.     

Bacillus thuringiensis delta-endotoxin: evidence that toxin acts at the surface of susceptible cells

Enzymically activated delta-endotoxin of Bacillus thuringiensis covalently bound to Sephadex beads, has the same effect on insect cells in tissue culture as free toxin. The effect is prevented by antitoxin antibody and heat denaturation and is not due to a nonspecific protein effect, the beads, or toxin released from the beads. The toxin, therefore, probably acts at the cell surface.     

On the formation of the myotomes in avian embryos. An experimental and scanning electron microscope study

Summary The formation of the myotomes to chick embryos was studied by using experimental and scanning electron microscope techniques. With the aid of the quail-chick marker system, it was demonstrated that the dermatome gives rise to the myotome plate. SEM studies support the conclusion that the myotome cells originate only from the edges of the dermatome.Supported by the Deutsche Forschungsgemeinschaft (Ch 44/2).     

Long-term depression of two primary immune responses induced by a single dose of 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC)

Summary 2 primary immune responses (anti-SRBC antibody response and allograft rejection) have been tested in mice at various time intervals after single doses of either DTIC or cyclophosphamide. The DTIC-induced immunodepression proved to be extremely long-lasting, being still detectable after 2 months.This work was supported by Project NO1-CB 64054 of the U.S.-Italy Cooperative Science Program.     

Dissolution of gallstones in hamsters by 3-hydroxy-3-methylglutaric acid

Summary Addition of 0.05–0.20% of 3-hydroxy-3-methylglutaric acid to a lithogenic hamster diet resulted in a marked reduction in the incidence of gallstones in hamsters.Supported, in part, by grants HL-03299, AG-00076 and T32 CA-09171 and a Research Career Award, HL-0734 from the National Institutes of Health.     

Immunocytology of alpha-endorphin positive hypothalamic fibers of Carassius auratus L. and Cyprinus carpio L.]

An immunocytological study of the basal hypothalamus of Carassius auratus and Cyprinus carpio with an alpha-endorphin antiserum shows the different nerve processes belonging probably to the immunoreactive cells of the lateral part of the N.L.T. According to their pathways, two main fibre tracts have been distinguished: many fluorescent fibres converge towards the pituitary gland into which they enter, other fibres run caudally through the tuber towards the nucleus tuberis inferior (Baumgarten and Braak) where they could not be followed further.     

Origin and course of an afferent component of the facial nerve within the central nervous system

Summary The mesencephalic nucleus of trigeminal gives rise to an afferent component of the facial nerve. This nucleus contains large unipolar afferent cell bodies which give rise to an axon which courses caudally through the brainstem and exits via the facial nerve to terminate distal to the stylomastoid foramen.This work was supported by National Aeronautics and Space Administration Grant No. NAS 9-15213.