Toxicity of Phytophthora infestans and Alternaria solani to chick embryos

None of the extracts and culture filtrates from growth of 9 races of Phytophthora infestans on living potato tissue and potato dextrose broth are toxic to chick embryos. Chloroform extracts of 4 out of 10 isolates of Alternaria solani grown on potato dextrose broth showed some toxicity to chick embryos.     

Further characterization of the effects of ultraviolet irradiation of the amphibian egg

UV-irradiation of the vegetal hemisphere of amphibian eggs leads to developmental abnormalities in neural morphogenesis. The possibility that the egg's transient sensitivity to irradiation could be due to pigmentation changes was examined in albino eggs. The tissue specificity of the effects of irradiation was analyzed by exchanging the ectoderm between irradiated and control embryos.     

Histochemical characterization of the red fibres in pigeon pectoralis muscle.

Red fibres of the pigeon pectoralis muscle showed high ATPase reaction at pH 9.4. Veronal-acetate pretreatment completely inhibited the ATPase reaction in these red fibres but not in type I fibres of the gastrocnemius. The former are type II red muscle fibres and hence are unlike type I red, the so-called slow-twitch muscle fibres.     

HCO-3-ATPase activity distribution in rat liver cell fractions prepared by zonal centrifugation.

Plasma membrane sheets prepared by zonal centrifugation of a premicrosomal pellet obtained from a rat liver homogenate are devoid of HCO-3-ATPase activity. Since the microsomal fraction is also lacking in this ATPase activity, it can be concluded that the HCO-3-ATPase is not involved in the secretion of HCO-3 into bile.     

Species-dependent stereospecific serum protein binding of the oral anticoagulant drug phenprocoumon.

13 mammalian species are classified into 3 clearcut groups with respect to the stereospecific serum protein-binding of phenprocoumon: 2 groups showing opposed stereospecific binding characteristics and a 3rd group exhibiting no stereospecific binding. Structural differences in the albumin molecule account for these stereospecific differences in serum protein-binding.     

Isoelectric focusing study of radiation damage to dry conalbumin.

The effect of gamma-rays on iron-free conalbumin was studied by isoelectric focusing technique. The results obtained can be interpreted on the basis of radiation-induced conformational changes of the macromolecules through a cleavage of disulphide bridges. Treatments with reagents acting on disulphide bridges lead to isoelectric focusing patterns that confirm this hypothesis.     

Neutral proteases in the guinea-pig lymphocytes.

Partial purification of neutral proteolytic enzymes in guinea-pig lymphocytes yielded 2 enzymes. Both enzymes were heat-labile and inhibited by thiol reagents. The molecular weights were more than 200,000 and 150,000-200,000, and optimal pH around 9 and 8, respectively.     

Influence of hydrocortisone on cytopathic effect of Newcastle disease virus and stability to freezing of vescicular stomatitis virus.

The presence of hydrocortisone in virus-infected cell cultures leads to enhancement of the syncytia forming ability of Newcastle disease virus and to production of vescicular stomatitis virus particles which loose their infectivity upon storage below 0 degrees C.     

Lipoprotein lipase activator deficiency in very low density lipoproteins in rat nephrotic syndrome.

Marked urinary loss of lipoprotein lipase activator in experimental rat nephrotic syndrome may be partly responsible for its deficiency in plasma very low density lipoproteins.     

High-affinity binding of beta-alanine to cerebral synaptosomes might involve glycine-receptors.

High-affinity, Na+-independent binding of beta-alanine to a synaptosomal fraction of rat brain was potently inhibited by glycine and by some other alpha-amino acids, but not by taurine or GABA. This binding mechanism, which was also sensitive to both bicuculline and strychnine, might involve synaptic receptors for both beta-alanine and glycine.