FITC标记的GSTP1蛋白在鼠巨噬细胞中的定位

GSTP1(GSTπ)是人体内谷胱甘肽-S-转移酶(GST)的一种重要的活性亚型,属于机体内源性蛋白,不会引起免疫原反应,在维持细胞的正常机能中发挥着重要作用,也是一种具有临床应用价值的抗氧化剂.本实验室在研究中首先发现GSTP1具有明显的抗炎作用,由此我们设想可以通过增加外源性的GSTP1蛋白来提高其在机体内的水平,以此增强机体的抗炎能力.本实验将薄层色谱扫描和SDS-PAGE相结合用于FITC标记蛋白质的检测,使用激光共聚焦显微镜和流式分析相结合的方法研究了外源GSTP1蛋白在RAW264.7巨噬样细胞和鼠腹腔巨噬细胞中的跨膜转位,结果显示外源GSTP1蛋白能够进入上述细胞.该研究为GSTP1蛋白作为直接使用的药物在抗肿瘤、抗炎、抗氧化等领域的应用提供了实验基础.     

Transmission of oxygen isotope signals of precipitation-soil water-drip water and its implications in Liangfeng Cave of Guizhou,China

According to systemically monitoring results of oxygen （hydrogen） isotope compositions of precipitation, soil waters, soil CO2, cave drip waters and their corresponding speleothems in Liangfeng Cave （LFC） in Guizhou Province, Southwest China, it is found that local precipitation is the main source of soil waters and drip waters, and that the amplitudes of those δ18O values of three waters （precipitation, soil water and drip water） decrease in turn in the observed year, which are 0‰ to -10‰, -2‰ to -9‰ and -6‰ to -8‰, respectively. Moreover, the δ18O values for three waters show a roughly simultaneous variation, namely, that those values are lighter in the rainy seasons, weightier in the dry seasons, and that the average δ18O value of drip waters is about 0.3‰ weightier than that of precipitation, which is modified by surface evaporation processes. We also find that oxygen isotope equilibrium is reached or neared in the formation processes of speleothems in LFC system, and that it is feasible to reconstruct paleotemperature and paleoprecipitation by using δ18O values of speleothems. However, it should be noted that surface evaporation would affect the oxygen isotope values in the study area.     

SHRIMP U-Pb zircon age of tuff at the bottom of the Lower Cambrian Niutitang Formation,Zunyi, South China

A SHRIMP U-Pb zircon geochronology study of the tuff at the bottom of the Lower Cambrian Niutitang Formation at Songlin, Zunyi, South China yielded a mean ^206Pb/^238U age of 518±5 Ma. It is significantly younger than the Re-Os ages of 537--542 Ma for the overlying polymetallic Ni-Mo-PGE-rich layer in the basal Niutitang Formation at Songlin, suggesting that the Re-Os ages might represent that of the original magma chamber for the Ni-Mo-PGE-rich layer rather than the formation age of the strata. The intra-basinal stratigraphic correlation also implies that the absolute age of the Chengjiang biota should be younger than 518±5 Ma. Our new result, together with the SHRIMP U-Pb zircon age for the K-bentonite in the Zhongyicun member of the Zhujiaqing Formation at the Meishucun section, Yunnan Province, provides a temporal constraint for the Lower Cambrian of the Yangtze Platform in South China.     

Three-dimensional fluorescence spectral characteristics of dissolved organic carbon in cave drip waters and their responses to environment changes: Four cave systems as an example in Guizhou Province,China

Understanding the responses of fluorescence spectral characteristics of cave drip waters to modern environment and climate changes is key to the reconstructions of environmental and climatic changes using fluorescence spectral characteristics of speleothems. The fluorescence spectral characteristics of dissolved organic carbon （DOC） in four active cave systems were analyzed with a three-dimensional （3D） fluorescence spectral analysis method. We found that the fluorescence types of DOC were mainly of fulvic-like and protein-like fluorescences, both in soil waters and cave drip waters. The intensity of fulvic-like fluorescence was positively correlated with the concentrations of DOC, suggesting that the DOC of cave drip waters was derived from the overlying soil layer of a cave system. Compared with the other cave systems, the variation range of the excitation and emission wavelengths for fulvic-like fluorescence of cave drip waters in Liangfeng cave system that had forest vegetation was smaller and the excitation wavelength was longer, while its fluorescence intensity varied significantly. By contrast, the excitation and emission wavelengths and fluorescence intensity for that in Jiangjun cave system that had a scrub and tussock vegetation showed the most significant variation, while its excitation wavelength was shorter. This implies that the variation of vegetation overlying a cave appears to be a factor affecting the fluorescence spectral characteristics of cave drip waters.     

三重抗干扰晶闸管触发隔离输出电路

设计了一种抗干扰能力极强的晶闸管触发隔离电路.光耦隔离器件的电源依靠脉冲变压器供电的设计,极大的提高了触发电路的抗干扰能力.     

行程时间可靠性研究

文章结合已有研究成果,提出了行程时间可靠性的概念,分析了基于供需随机性构建的路径行程时间可靠性、OD对行程时间可靠性及系统行程时间可靠性模型,最后构建了基于最可能状态的行程时间可靠性近似算法。     

高强钢铰线网—聚合灰浆加固梁斜截面承载力计算方法研究

对采用"高强不锈钢绞线网-渗透性聚合物砂浆"受剪加固梁的斜截面承载力的计算方法进行探讨。文通过对混凝土梁及其加固梁试验结果的对比分析发现:按《规范》(GB10050-2002)计算加固梁的抗剪承载力安全余度比未加固梁的安全余度小得多,其主要原因是加固体中采用的钢铰线箍筋的强度过高,得不到充分利用;其次是由于加固体混凝土与原梁体混凝土受力和变形的不同步。因此文对抗剪加固梁的抗剪承载力计算公式进行了修正:限制加固体内箍筋的设计强度,折减加固体混凝土的抗剪承载力。从修正公式计算值与试验结果的比较看,修正后加固梁承载力的安全余度与未加固混凝土梁基本接近。     

低功率激光照射对急性骨骼肌钝挫伤大鼠自由基代谢的影响

采用美国FDA认可的ErchoniaEML（ML2）型GaAlAs低功率激光器,对急性骨骼肌钝挫伤模型大鼠进行治疗,观察低功率激光照射对损伤骨骼肌再生与修复过程中自由基代谢的影响,探讨低功率激光对骨骼肌损伤的防治作用．结果发现,低功率GaAlAs激光照射能显著提高受损肌肉的SOD活性,增强抗氧化能力,减轻氧化损伤．     

闭管回流——分光光度法测定化学需氧量(COD)

采用闭管回流——分光光度法测定水样的化学需氧量(COD)与标准(经典)回流法测定结果一致,该方法适用于环境监测中污水水质的检测。方法的准确度和精确度符合测试要求,且试剂用量少、成本低、无需滴定、操作简便,易实现数据在线处理,尤其适合室内外批量测试。文中考察了影响测试结果的因素,确立了试剂配制方法和操作程序。     

Improving the accuracy of GRACE Earth's gravitational field using the combination of different inclinations

In this paper,the GRACE Earth's gravitational field complete up to degree and order 120 is recovered based on the combination of different inclinations using the energy conservation principle.The results show that because different inclinations of satellite are sensitive to the geopotential coefficients with different degrees/and orders m.the design of GRACE exploiting 89° inclination can effectively improve the accuracy of geopotential zonal harmonic coefficients.However,it is less sensitive to the geopotential tesseral harmonic coefficients.Accordingly.the second group of GRACE exploiting lower inclination is required to determine high-accurately the geopotential tesseral harmonic coefficients and cover the shortage of the single group of GRACE exploiting 89° inclination.Two groups of GRACE individually exploiting 89° (82°-84°)inclinations are the optimal combination of the Earth'S gravitational field recovery complete up to degree and order 120.In the degree 120,the joint accuracy of cumulative geoid height based on two groups of GRACE individually exploiting 89° and 83° inclinations is averagely two times higher than the accuracy of a group of GRACE exploiting 89° inclination.