Isolation,purification and characterization of a new organphosphorus hydrolase OPHC2

A bacterium with the capability of degrading organphosphorus, identified as Pseudomonas pseudoaicaligenes, is isolated from OP-treated soil. The organphosphorus hydrolase OPHC2 from this bacterium has been purified and characterized. OPHC2 has optimum activity for the reaction at 65℃ and pH 9.0 with methyl parathion as a substrate, it also shows good thermal and pH stability. Most metal ions and chemicals have no effect on the activity of OPHC2. The analyses of nucleotide sequence encoding OPHC2 and amino acid sequence of OPHC2 show that there are lower homologies with those of organphosphorus hydrolase reported in GenBank.     

Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer

Aberrant WNT pathway signaling is an early progression event in 90% of colorectal cancers. It occurs through mutations mainly of APC and less often of CTNNB1 (encoding beta-catenin) or AXIN2 (encoding axin-2, also known as conductin). These mutations allow ligand-independent WNT signaling that culminates in abnormal accumulation of free beta-catenin in the nucleus. We previously identified frequent promoter hypermethylation and gene silencing of the genes encoding secreted frizzled-related proteins (SFRPs) in colorectal cancer. SFRPs possess a domain similar to one in the WNT-receptor frizzled proteins and can inhibit WNT receptor binding to downregulate pathway signaling during development. Here we show that restoration of SFRP function in colorectal cancer cells attenuates WNT signaling even in the presence of downstream mutations. We also show that the epigenetic loss of SFRP function occurs early in colorectal cancer progression and may thus provide constitutive WNT signaling that is required to complement downstream mutations in the evolution of colorectal cancer.     

面向对象技术在流域洪水预报系统中的应用

通过对现有的洪水预报系统的分析,确定了复用率低以及难于扩展是目前洪水预报应用系统在实现方面存在的普遍问题。利用面向对象的Java语言作为开发工具,进行洪水预报应用系统的开发,使系统具有较强的可扩展性和复用能力,能有效地缩短新系统的开发周期,因而是进行洪水预报系统开发的有效工具。     

Large eddy simulation of the gas-particle turbulent wake flow

To find out the detailed characteristics of the coherent structures and associated particle dispersion in free shear flow, large eddy simulation method was adopted to investigate a two-dimensional particleladen wake flow. The well-known Sub-grid Scale mode introduced by Smagorinsky was employed to simulate the gas flow field and Lagrangian approach was used to trace the particles. The results showed that the typical large-scale vortex structures exhibit a stable counter rotating arrangement of opposite sign, and alternately form from the near wall region, shed and move towards the downstream positions of the wake with the development of the flow. For particle dispersion, the Stokes number of particles is a key parameter. At the Stokes numbers of 1.4 and 3.8 the particles concentrate highly in the outer boundary regions. While the particles congregate densely in the vortex core regions at the Stokes number of 0. 15, and the particles at Stokes number of 15 assemble in the vortex braid regions and the rib regions between the adjoining vortex structures.     

完全3部图的一类同构分解

本文构造性地证明，对于完全3部图 G（X，Y，Z；E），如果边数能被正整数t整除，且｜X｜, ｜Y｜和｜Z｜三个数之一也能被t整除，则完全3部图可分解为t个同构因子，从而证实了Harary “3部图猜想”的部分结论。     

Effects of calcineurin on LTP of rats <Emphasis Type="Italic">in vivo</Emphasis>

Calcineurin (CN) is thought to play a role in the synaptic plastivity and long-term potentiation (LTP) in the hippocampus. Based on two LTP models in vivo, a specific inhibitor cyclosporin A (CsA) of CN was observed, which affected LTP in the hippocampal dentate gyrus of the rats. The results indicated that CsA blocked LTP induced by high frequency stimulation (HFS) partly, but it had no effect on the decrease of the onset and peak latency of population spikes (PS) except that it reduced the increase of the amplitude after HFS. On the other hand, CsA blocked LTP induced by ginsenosides (GSS) completely. It suppressed the GSS-enhanced amplitude of PS reversibly and blocked the decrease of the peak latency of PS induced by the GSS. These results suggest that the postsynaptic CN plays a role in the induction of LTP in the hippocampus of the rats.     

位移连续变更与极限状态函数的连续生成

基于我国《建筑结构设计统一标准》的极限状态设计原则,结构物不允许出现影响正常使用的外观变形,提出了刚架结构位移连续变更方法·用数值分析与递推公式相结合的方法,实现连续变更结构的变形极限状态函数的连续生成·重分析不必重新形成结构刚度矩阵,不必反复求逆矩阵,使得识别系统主要失效模式的方法得以简化,有效地提高了计算时效,并为刚度可靠性分析提供了新方法·算例验证了方法的正确性及实用性·     

The earliest known eutherian mammal

The skeleton of a eutherian (placental) mammal has been discovered from the Lower Cretaceous Yixian Formation of northeastern China. We estimate its age to be about 125 million years (Myr), extending the date of the oldest eutherian records with skull and skeleton by about 40 50 Myr. Our analyses place the new fossil at the root of the eutherian tree and among the four other known Early Cretaceous eutherians, and suggest an earlier and greater diversification of stem eutherians that occurred well before the molecular estimate for the diversification of extant placental superorders (104 64 Myr). The new eutherian has limb and foot features that are known only from scansorial (climbing) and arboreal (tree-living) extant mammals, in contrast to the terrestrial or cursorial (running) features of other Cretaceous eutherians. This suggests that the earliest eutherian lineages developed different locomotory adaptations, facilitating their spread to diverse niches in the Cretaceous.     

移动通信Rician信道中的多普勒影响分析

在研究分析了陆地移动通信Rician多径信道中因存在较强支配路径信号入射以及入射路径空间位置发生变化对Doppler频谱密度所产生影响的基础上 ,指出在实际无线传播环境中的一般性Doppler频谱密度通常应为非对称的 ,并且给出了相应的理论分析和仿真结果     

道德、法律与公民意识

自改革开放以来,我国在一直大力加强法制建设的同时却忽视了道德秩序的建立。面对社会现在的“道德危机”和“法律纸面化”问题,我们必须提高道德与法律的内化力－公民意识。采取灵活多样的有效形式,推进中国法治现代化进程。