Effect of hydrogen peroxide on the binding of Merocyanine 540 to human erythrocytes

The fluorescent dye Merocyanine 540 (MC540) is often used as a probe to monitor the molecular packing of phospholipids in the outer leaflet of biomembranes. In a previous study we showed that the increased staining of erythrocytes with a perturbed membrane structure was mainly due to an increase in the fluorescence yield of cell-bound MC540, rather than to an increase of the number of bound molecules. Erythrocytes and ghosts exposed to continuous fluxes of H₂O₂ exhibited pronounced lipid peroxidation. Further, red blood cells subjected to this form of oxidative stress also showed increased staining with MC540. It appeared that this was caused by a strong increase in binding of MC540, together with a slight red shift of the fluorescence emission maximum and a small increase in the fluorescence yield of bound MC540. The changed MC540 binding characteristics were not observed when lipid peroxidation was suppressed by the presence of the antioxidant BHT in the incubation medium. However, open ghosts exposed to H₂O₂ showed no increase of MC540 binding, excluding a direct involvement of lipid peroxidation. Measurement of fluorescence emission spectra and gel filtration studies showed that MC540 can bind to H₂O₂-exposed hemoglobin. Experiments with erythrocytes lysed in hypotonic medium after exposure to H₂O₂ revealed that peroxidation of lipids with H₂O₂ induced a non-specific permeabilization of the plasma membrane to MC540, thereby allowing MC540 to bind to the oxidatively denatured, more hydrophobic hemoglobin. These results indicate that conclusions about packing of phospholipids in the outer leaflet of the membrane based on increased MC540-staining should be drawn with care. Received 27 September 1996; received after revision 5 November 1996; accepted 27 November 1996     

平台用钢微观组织对其分层面上解理断裂应力的影响

利用专门设计的厚度方向缺口四点弯曲试样，结合相应的弹塑性有限元计算结果，在液氮温度下测取了一种海洋平台用钢的６种微观组织分层面上的解理断裂应力σ＊ｆ．结果显示，均匀细小的铁素体＋贝氏体组织具有比铁素体＋带状珠光体为高的σ＊ｆ值．对于同一终轧温度，轧后冷速愈高，其得到的微观组织之σ＊ｆ值愈高．而对于同一轧后冷却制度，则终轧于Ａｒ３与再结晶停止温度之间者其得到的微观组织具有最高的σ＊ｆ值．讨论了各微观组织组成相的作用     

Two classes of metabolites fromTheonella swinhoei are localized in distinct populations of bacterial symbionts

The marine spongeTheonella swinhoei (lithistid Family Theonellidae, Order Astrophorida) has yielded many important, bioactive natural products, most of which share structural features with bacterial natural products. The presence of microbial symbionts inT. swinhoei has been reported, and it was originally suggested that the cytotoxic macrolide swinholide A and many of the bioactive cyclic peptides fromT. swinhoei were all produced by symbiotic cyanobacteria. By transmission electron microscopy, we found four distinct cell populations to be consistently present inT. swinhoei: eukaryotic sponge cells, unicellular heterotrophic bacteria, unicellular cyanobacteria and filamentous heterotrophic bacteria. Purification and chemical analyses of each cell type showed the macrolide swinholide A to be limited to the mixed population of unicellular heterotrophic bacteria, and an anti-fungal cyclic peptide occurred only in the filamentous heterotrophic bacteria. Contrary to prior speculation, no major metabolites were located in the cyanobacteria or sponge cells.     

从高放废液中去除超铀元素的TRPO流程热实验

阐述了从高放废液中去除超铀元素的ＴＲＰＯ流程热实验。热实验是用真实动力堆的 高放液进行的，取得了很好的效果。超铀元素镅、镎、钚和铀的去污系数分别大于 ３ ０００，４ ０００， ９５０和 ７ ０００。 ＴＲＰＯ流程在去除超铀元素的同时，还能去除对放射性废物长期危害起决定作用 的９９Ｔｃ。实验结果表明ＴＲＰＯ流程具有去污效率高，反萃效果好，锕系组分之间交叉污染小和试 剂成本低等诸多优点。ＴＲＰＯ流程是一个有效的从高放废液中去除超铀元素的流程。     

多元条件密度函数的投影追踪估计方法

由多元条件密度函数fYX(yx)(x∈Rp)可以知道许多被解释变量Y对解释变量X的回归关系的信息,其条件期望就是回归函数,条件方差就是回归误差项的条件方差.为了克服高维空间数据稀松性带来的估计上的困难,提出多元条件密度函数的投影追踪估计方法,通过最小化Ku llback-Le ib ler距离,得到了最优初始条件密度函数和每一步的增量函数和方向向量,还给出了估计步骤及其终止法则.     

纳米复合彩色薄膜的制备

以正硅酸乙酯、乙醇、生物染色剂为主要原料 ,通过溶胶 -凝胶法制备了掺有有机染料的SiO2 溶胶 ,利用提拉法在普通玻璃上成功镀制了均匀透明、色泽丰富的彩色涂层 ,该涂层颜色在高温下会彻底分解消失 ,实现了玻璃的可回收利用 .运用光谱分析、扫描探针显微镜研究了薄膜的结构 ,以及工艺参数对薄膜在可见光区光谱性能的影响 .     

世界文化和不可知论的政见——一个致力于跨文化融洽交流的计划

就世界文化课程内容的具体设置,及联合大学与传媒以更好地实现课程积极目标等诸问题进行探讨.通过多层次的论证强调指出人们始终持有相异或相冲突的宗教、哲学以及意识形态,尚没有一种普世真理足以使世间所有人心悦诚服.即便是广泛接受和运用中的科学知识,其发展亦受阻于人类本身的局限而不可确定.尽管科学在前沿问题研究上竞争激烈,也仍然无法回答和消除所有的问题和差异.因此,从全球或泛人类立场观之,人类现有的知识与理解能力具有不确定和不完整的特点,而这样的特点浸透在所有的具体文化信仰之中.认识到共同局限性的人们--尤其是政界的领导人--亟需摒弃极端的文化分离思想,以更宽容理解的心态思考和行动,这样才能避免不同民族及不同国家间的不断冲突和互相伤害.     

Nuclear Technique and Agriculture in China

Nuclear technique is a powerful scientific tool in agricultural research, an area with fruitful achievements in China. Application of nuclear technique in agriculture (Nuclear Agricultural Sciences) based on the development of related science and technology is a high-tech area, and also a significant aspect of non-electrical power application of nuclear technique. As an important component of agricultural science and technology, Nuclear technique application in agricultural field has achieved a lot and made remarkable contributions to the economic, social and ecological development of China. The current paper reviews the achievements, and presents the situation of the application of nuclear technique in the Chinese a-griculture briefly. The author strongly suggests that Chinese     

Growth of Magnetite Nanoparticles under Magnetic Fields

1 Introduction Over the past several years, the preparation and characterization ofnanoscale magnetic materials, especially one-dimensional (1D) nanostructure, have attracted much attention as the nanomaterials would allow investigating the fundamental aspects of magnetic-ordering phenomena in magnetic materials with reduced dimensions and could lead to new potential applications such as data storage technology[1-6].     

Experimental Study and Numerical Simulation of the Casting-Forging Complex Near Net Forming of Alternator Claw-pole

As a newly developed precision technology, casting-forging complex near net forming process is utilized to produce complex components with a short lead time, low cost and high precision, thus to accelerate the response speed of the market and enhance the competitive power of products. In this paper, the casting-forging complex near net forming process of alternator claw pole was developed and investigated with a combination of experimental and numerical simulation method. Qualified near net workpiece was manufactured, mechanical parameter and relative field information during the forming process was also obtained. While the alternator claw-pole is processed with this technology, the forming force is small, the process is short and the quality of forgings is perfect. Therefore, the complex casting-forging near net forming process of claw-pole is an energy and material saving technology, which will have a vast developing and application prospect in the future.