Sinorhizobium meliloti lsrB is involved in alfalfa root nodule development and nitrogen-fixing bacteroid differentiation

Rhizobia interact with host legumes to induce the formation of nitrogen-fixing nodules, which is very important in agriculture and ecology. The development of nitrogen-fixing nodules is stringently regulated by host plants and rhizobial symbionts. In our previous work, a new Sinorhizobium meliloti LysR regulator gene (lsrB) was identified to be essential for alfalfa nodulation. However, how this gene is involved in alfalfa nodulation was not yet understood. Here, we found that this gene was associated with prevention of premature nodule senescence and abortive bacteroid formation. Heterogeneous deficient alfalfa root nodules were induced by the in-frame deletion mutant of lsrB (lsrB1-2), which was similar to the plasmid-insertion mutant, lsrB1. Irregular senescence zones earlier appeared in these nodules where bacteroid differentiation was blocked at different stages from microscopy observations. Interestingly, oxidative bursts were observed in these nodules by DAB staining. The decreased expression of lipopolysaccharide core genes (lpsCDE) was correspondingly determined in these nodules. S. meliloti lipopolysaccharide is required for suppression of oxidative bursts or host cell defense. These findings demonstrate that the S. meliloti lsrB gene is involved in alfalfa root nodule development and bacteroid differentiation by suppressing oxidative bursts or defense responses in host cells.     

Discussion on the cantilever casting construction method of concrete crack prevention and control

随着我国建设高峰的来临,悬浇法施工混凝土桥梁越来越多,相伴而来的桥梁混凝土开裂事故也越来越多.通过对施工工艺不当所造成的裂纹原因分析,制定了切实可行的预防及处理措施,对以后同类桥梁施工具有指导意义.     

浅析电力变压器的事故处理

电力变压器是电力系统中很重要的设备,一旦发生事故,将造成很大的经济损失.分析各种电力变压器事故,找出原因,总结出处理事故的办法,把事故损失控制在最小范围内,尽量减少对系统的损害.     

省级实验示范中心在高校人才培养中的作用

对高校而言,实验教学是理论教学以及科研的基石,是深化理论知识,将间接经验与直接经验相结合的桥梁。论文结合安徽医科大学基础医学院病原与免疫学实验室的成长历程,阐述了省级实验示范中心在高校人才培养中的积极作用,体现在省级实验示范中心建设的引领下,实验中心本着提高实验教学水平和人才培养质量为目标,逐步实现提高实验室建设和管理水平,充分发挥实验示范中心的示范引领作用,以期全面提高高校的实验教学水平,并为高校进一步的实验教学改革创造条件。     

Comparison study of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres

To compare two microspheres of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin loaded theophylline as pulmonary sustained drug delivery carriers, the characteristics and ciliotoxicity were studied. The drug loadings of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 21.09% and 21.42%, and the encapsulation efficiencies were 91.40% and 92.80%. The distributions of 50% (d _0.5) of chitosan/β-cyclodextrin and carboxymethyl chitosan/β-cyclodextrin microspheres were 4.89 and 5.83 μm, respectively. Both microspheres showed spherical shape with smooth or wrinkled surfaces. FT-IR of chitosan/β-cyclodextrin microspheres demonstrated that theophylline had formed hydrogen bonds with chitosan and β-cyclodextrin, while for the carboxymethyl chitosan/β-cyclodextrin microspheres theophylline had interaction with carboxymethyl chitosan. The moisture absorption showed that an equilibrium was reached within 24 h. The two microspheres possessed better adaptability. In vitro release of theophylline from chitosan/β-cyclodextrin microspheres was slower than that from carboxymethyl chitosan/β-cyclodextrin microspheres at pH 6.8. Biography: LI Ruobao(1968–), male, Associate professor, research direction: foundation research of respiratory system.     

Inferring requirement goals from model implemented in UML

UML is used widely in many software development processes. However, it does not make explicit requirement goals. Here is a method tending to establish the semantic relationship between requirements goals and UML models. Before the method is introduced, some relevant concepts are described.     

Framework for dissection of complex cytonuclear epistasis by a two-dimensional genome scan

Epistasis between cytoplasmic and nuclear genes is the primary genetic component of complex quantitative traits.Genetic dissection of cytonuclear epistasis is fundamentally important to understand the genetic architecture of complex traits.In this study,a two-dimensional genome scan strategy was employed to evaluate the contribution of cytoplasm,quantitative trait loci (QTL),QTL×QTL interactions and QTL×QTL×cytoplasm interactions to the phenotypic variation.The p-value and parameter value for each genetic effect were calculated by multiple regression analysis.A stepwise approach was suggested to build confidence in candidate QTL on the basis of q-value estimation,false discovery rate calculation and Bonferroni adjustment.A fine-scale grid scan strategy was proposed for further analysis of peaks of interest.Plant height in maize was used as an example to illustrate the efficiency of the two-dimensional genome scan strategy.     

梁启超与孙中山民族主义思想之比较

民族主义作为中国近代史上最具广泛感染力与强大推动力的一种社会政治思潮,曾是那个时代各派人物高擎的一面旗帜.作为中国近代民族主义的先行者、实践者,梁启超与孙中山都曾集结在这面旗帜之下,以各自思想所富有的丰富内容、深刻内涵、鲜明特色而影响着当时的民族独立解放运动.     

翻译家余光中研究

当代诗坛大家、散文健将和著名评论家余光中同时还是一名优秀的翻译家。结合其翻译理论与实践从翻译家的定位对其进行分析,不仅有助于从个案的角度加深对余先生及其文学成就的研究,还能从普遍的角度为译者修养、翻译理论与实践的关系等问题提供参考。     

高速重轨矫正圈用9Cr2MoRE钢的开发研究

通过添加不同含量的稀土元素,对比研究稀土含量对9Cr2Mo钢奥氏体晶粒度、相变点、抗拉强度及耐磨性的影响.研究结果表明,稀土元素可以有效细化9Cr2Mo钢的奥氏体晶粒,当稀土加入量为0.10%时,晶粒长大速率降低最明显.添加质量分数为0.1%的稀土后,9Cr2Mo钢的抗拉强度明显提高,塑性和延展性也有所改善.加入稀土元素后,9Cr2Mo钢在磨损过程中可以避免出现大块疲劳剥落现象,提高了耐磨性.