GCC box in<Emphasis Type="Italic">Arabidopsis PDF1.2</Emphasis> promoter is an essential and sufficient cis-acting element in response to MeJA treatment

The expression of Arabidopsis PDF1.2 gene isregulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element in PDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression o fPDF1.2 was confirmed by using the upstream -1.86 kb fragment of PDFI.2 gene. Secondly, the upstream -300— -243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the -300— -243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box in PDFI.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4xGCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box in PDFI.2 is an essential and sufficient element to confer MeJA induction.     

数据报协议远程网的排队模型

本文对数据报协议远程网进行了性能分析．本文首次指出：数据报协议远程网的排队模型是一种自助服务系统模型．     

新型双希夫碱化合物的合成及表征

以对硝基苯甲酸、乙二醇、1,4-丁二醇及水杨醛等化合物为原料,经多步反应合成2种新型双希夫碱化合物(1c、2c),利用红外光谱、紫外光谱、核磁氢谱、元素分析对2种双希夫碱化合物进行结构表征.     

茶文化主题观光农业园规划探讨——以渝北石盘河茶文化主题观光农业园规划为例

以渝北石盘河茶文化主题观光农业园规划为例,探讨了茶文化主题表达的路径.在进行茶文化主题观光农业园规划时,应重点把握茶文化主题观光农业园与一般农业园规划的区别,这样才能突出茶文化主题观光农业园的独特之处.     

臭氧脱除蔬菜中残留有机磷农药的研究

用臭氧处理蔬菜中残留的甲胺磷、敌敌畏、氧化乐果,研究臭氧对蔬菜中常见有机磷农药脱除效果.结果表明,臭氧处理蔬菜中残留的甲胺磷、氧化乐果、敌敌畏3种有机磷农药效果明显,经10 mg/L臭氧处理30 min后,蔬菜中残留的有机磷农药脱除率在85%以上.     

Φ50 mm英标螺纹钢筋的研发与生产

文章介绍了莱钢集团棒材厂轧钢生产线研发生产Ф50mm英标螺纹钢筋的技术准备、孔型设计、轧辊加工、穿水工艺设计及钢筋穿水后的金相组织变化分析。     

Culinary archaeology: Millet noodles in Late Neolithic China

Noodles have been a popular staple food in many parts of the world for at least 2,000 years, although it is debatable whether the Chinese, the Italians or the Arabs invented them first. Here we analyse a prehistoric sample of noodles contained in a well preserved, sealed earthenware bowl discovered in the Late Neolithic archaeological site of Lajia in northwestern China. We identify millet as the source of the abundant seed-husk phytoliths and starch grains present in the vessel. This shows that the conversion of ground millet flour into dough that could be repeatedly stretched into long, thin strands for the preparation of boiled noodles was already established in this region 4,000 years ago.     

浅谈建筑安装工程造价结算价的审核

文章主要从影响工程结算的因素、做好结算审核的前期工作、结算审核的主要方法、结算审核时应注意的问题、提高结算审核人员的素质要求这几个方面进行阐述,以公正合理地做好工程结算审核。     

浅析教育机智

教育机智,指教师在教育过程中对意外情况准确、恰当、迅速地作出判断,随机应变、恰到好处地采取果断措施的能力.教育机智是一种可观察的行为表现,是教师对每个孩子的爱.     

如何让作文语言更美丽

文学的魅力靠语言,鲜活的、具有美感的文字是惹人喜爱的.文章针对如何让作文语言更美丽作一浅谈.