鲈鱼cxcr4 cDNA克隆和序列分析

趋化因子受体(cxcr4)是一类重要的免疫调节因子受体,对原始生殖细胞的迁移和存活具有十分重要的作用。在对脊椎动物cxcr4进行初步生物信息学分析基础上设计引物,采用RT-PCR和RACE相结合的方法从鲈鱼卵巢克隆了cxcr4a全长和4b部分cDNAs序列,并预测其编码的氨基酸序列。结果表明,由于鱼类基因组经历一次特有的复制,cxcr4在鱼类存在两个复制基因。鲈鱼cxcr4a cDNA全长为1432 bp,编码311个氨基酸;cxcr4b部分cDNA片段长为905 bp,编码285个氨基酸。将鲈鱼cxcr4序列与所有已克隆的硬骨鱼类进行同源性比较,结果显示硬骨鱼类保守性较高,所有已经克隆的cxcr4按照进化地位的不同成簇聚类,表现出了较高的同源性。     

基于神经网络的车辆交通协调性评价模型

为了研究自动驾驶汽车交通协调性的主客观映射评价模型,以高速公路匝道汇入为研究场景,首先基于自然驾驶数据的交互样本数据,以自车平均行驶速度、并线时刻侧向速度、并线时刻车头TTC、并线时刻两车相对侧向速度和对手车减速程度等客观指标数据和交通协调性主观评价结果作为模型输入和输出,构建映射评价模型;然后设计2×2交叉对比实验,并分析数据预处理方法及神经网络类型对评价模型效果的影响。研究结果显示,基于线性函数归一化处理的BP神经网络模型和Dropout神经网络模型总精度分别为95.71%和80.00%,基于阶梯函数归一化处理的BP神经网络模型和Dropout神经网络模型总精度分别为94.60%和73.25%。由此可见,模型评价效果较好,所建立的客观表征指标集能够较好地表达专家对车辆交通协调性表现的评价。在建模方法方面,基于BP神经网络的映射评价模型的表现优于Dropout神经网络模型,能够根据客观数据更准确地得到符合专家评判标准的评价结果。在样本数据预处理方法方面,线性函数归一化处理方法能够在消除数据间数量级差异的同时保留客观表征指标集数据在不同样本之间的差异,能提高映射评价模型的评价精度。因此...     

Effect of H2S concentration on the corrosion behavior of pipeline steel under the coexistence of H2S and CO2

The effect of H2S concentration on H2S/CO2 corrosion of API-X60 steel was studied by scanning electron microscopy, a weight-loss method, potentiodynamic polarization tests, and the electrochemical impedance spectroscopy technique. It is found that the cor-rosion process of the steel in an environment where H2S and CO2 coexist at different H2S concentrations is related to the morphological structure and stability of the corrosion product film. With the addition of a small amount of H2S, the size of the anode reaction region is de-creased due to constant adsorption and separation of more FeS sediment or more FeHS＋ions on the surface of the steel. Meanwhile, the dou-ble-layer capacitance is diminished with increasing anion adsorption capacity. Therefore, the corrosion process is inhibited. The general cor-rosion rate of the steel rapidly decreases after the addition of a small amount of H2S under the coexistence of H2S and CO2. With a further increase in H2S concentration, certain parts of the corrosion product film become loose and even fall off. Thus, the protection provided by the corrosion product film worsens, and the corrosion rate tends to increase.     

收敛正项级数余项r_n的最优估计

对收敛正项级数余项r_n的估计有个优劣问题,本文对这个问题作了研究。首先给出正项级数余项r_n的最优估计概念,并指出这种最优估计的基本性质和意义,证明最优估计的两个充要条件,还给出了三个充分性定理(判别法)。最后的例子是判别法的应用和最优估计概念的实例说明。     

Enhancive effect of N,N′-dinitrosopiperazine on inducing precancerous lesion on nasal and/or nasopharyngeal epithelia of TgN(p53mt-LMP1)/HT mice

Objective: To investigate the enhancive effect of N,N′-dinitrosopiperazine (DNP) on induced carcinogenesis in nasal and/or nasopharyngeal epithelia among TgN(p53mt-LMP1)/HT transgenic mice to examine the underlying mechanism for the development of nasopharyngeal carcinoma (NPC). Methods: TgN(p53mt-LMP1)/HT transgenic mice and the same strain of C₅₇BL/6J wild-type mice both at the age of 5 months were randomly divided into 2 groups in parallel, respectively, i.e., TgN(p53mt-LMP1)/HT cancerous lesion-inducing group (TI), TgN(p53mt-LMP1)/HT control group (TC), C₅₇BL/6J cancerous lesion-inducing group (CI), and C₅₇BL/6J control group (CC). TI and CI mice were treated only with DNP for 16 weeks, twice each week, while TC and CC mice were given the same volume of saline as controls. At the end of treatment, animals were sacrificed to collect epithelial tissue samples from nasal cavity and nasopharynx for pathohistological evaluation by haematoxylin and eosin (HE) staining and for determination on the expression of TRAF2, c-Jun, and p16 by immunohistochemistry. Results: Atypical hyperplasia was more significant in the samples of TI than in those of TC, CI, and CC, with the rates of lesions being 90%, 10%, 0, and 0 (P<0.01) respectively, though DNP was used alone in a much shortened inducing period at less dosage and without the use of carcinogenic promoter 12-O-tetradecanoylphorbol-13-acetate as usual. The expressions of tumor necrosis factor (TNF) receptor-associated factor 2 (TRAF2) and c-Jun in these samples were significantly up-regulated in TI (P<0.01), while the expression of p16 was significantly lower in TI than in the other groups (P<0.01). Conclusion: TgN(p53mt-LMP1)/HT mice hold inherited constitutional defect in immune surveillance function, which can be aggravated by environmental carcinogens, such as DNP used even though in a much less strength. The enhanced carcinogenesis-inducing effect of DNP on TgN(p53mt-LMP1)/HT mice should be closely associated with abnormal signaling of activator protein-1 (AP-1) pathway, especially up-regulated expressions of TRAF2 and c-Jun, and down-regulated expression of p16.     

相对论多时间非平衡态系综

提出了在狭义相对论框架中合理的新的相对论多时间非平衡态系综．依此系综，多时间关联的非均匀体系和均匀体系的关联动力学理论可以严格地建立起来．它比传统的多时间系综理论简单、方便．     

绢纺生产用抗静电剂的研究——SS1与SS2绢纺抗静电剂

本文对绢纺生产用抗静电剂的配方进行了研究,研制出SS1及SS2绢纺抗静电剂。应用表明,采用此类抗静电剂,不但降低了生产中的静电,而且提高了梳折;在圆梳系统中提高了3.23％,在精梳系统中提高了3.1％;同时精绵的长度和强力也有所增加,从而提高了经济效益。     

石英管烧结成型设备的微机控制

介绍石英管烧成型设备－－气炼机的微机控制系统，系统采用双ＣＰＵ单片微机微机控制四台步进电机以完成气炼机的多种复杂运动，文中介绍了控制系统的硬件和软件结构。     

Zr50.5Cu36.5Ni4Al9块体非晶合金低温动态压缩特性

通过铜模铸造法制备Zr_50.5Cu_36.5Ni₄Al₉块体非晶合金. 利用分离式霍普金森杆（SHPB）,S-4800型扫描电镜等测试分析手段,研究Zr_50.5Cu_36.5Ni₄Al₉块体非晶合金在室温（25℃）和低温下（-40℃）动态压缩特性和断口形貌特征. 结果表明:在动态压缩载荷作用下,Zr_50.5Cu_36.5Ni₄Al₉块体非晶合金抗压强度随载荷增加而降低. 在-40℃低温条件下,动态抗压强度具有低温敏感性. 在室温条件下,Zr_50.5Cu_36.5Ni₄Al₉块体非晶合金断口微观形貌特征主要是脉状花样,伴随裂纹和非晶合金熔化现象;在-40℃ 低温条件下,Zr_50.5Cu_36.5Ni₄Al₉块体非晶合金微观形貌特征主要是鱼骨状花样.      

基于共词网络的雾霾事件下微博关注话题差异性——以中国北方雾霾严重地区典型城市为例

雾霾对人体健康、生态环境及交通运输等方面影响重大,雾霾来临时,人们会在微博等媒体上表达对雾霾的关注。微博中蕴含了网民对雾霾的关注话题及心理反映,通过微博掌握微博网民对于雾霾的相关反应,有助于城市管理者有针对性的调节雾霾情形下的舆论宣传和资源调配。考虑到微博文本中相同词语共现频率越高就越倾向于表达同一主题,采用基于社区的共词网络法挖掘微博话题,首先,采集2017年冬季以"雾霾""空气"等为关键词的微博数据,利用TF-IDF(term frequenly-inverse document frequency)算法对预处理之后的微博数据提取关键词,并获取关键词间的共现关系,然后基于共现关系构建共词网络,根据Louvain算法探测话题社区,最后,以中国雾霾污染最严重的华北地区、华东地区、东北地区7个典型城市为研究区,结合关键词节点的Pagerank值,分析各城市共词网络话题社区的差异程度。实验结果表明,不同城市对于雾霾事件的关注既有相同点也有差异性。研究结果对雾霾情形下城市差异化管理具有一定的理论指导作用。