Aromatase and steroid sulfatase mRNA expression in fine needle aspirates from benign and malignant breast disorders

The fine needle aspiration (FNA) test is a convenient and tolerable technique with minimal invasion that is accepted by most women.Local estrogen synthesis depends mainly on the aromatase and steroid sulfatase pathways that are believed to play important roles in breast carcinogenesis.However,little is known about the level of aromatase and steroid sulfatase mRNA expression in FNA samples which contain only small amounts of tissue.The nested Q-PCR assay has been proven to be a highly sensitive and specific method to assess the aromatase expression of breast tissue.In this study,aromatase and steroid sulfatase mRNA expression in 74 patients with benign or malignant disorders was evaluated and compared using nested Q-PCR and non-nested Q-PCR assays.The expression levels were analyzed and correlated with clinical parameters.No difference in the aromatase expression levels between nested and non-nested Q-PCR was noticed.Age and aromatase mRNA expression level were two independent risk factors for breast cancer (P=0.04 and P=0.00,respectively),while menopausal status and steroid sulfatase mRNA expression levels were not associated with breast cancer.This study showed that both nested and non-nested Q-PCR assays were effective methods for research using FNA breast samples.     

一类具有状态约束的线性二次最优控制问题

研究具有状态积分不等式约束的线性二次控制问题，证明了最优控制的存在性和唯一性，推导出最优控制的反馈形式。     

NS导向提馏塔板的流体力学性能

介绍了新开发成功的提馏专用塔板－－NS导向提馏塔板。该塔板是在充分考虑提馏段的具体要求，吸取各种导向精馏塔板的优点，如BVT蝶形浮阀，导向浮阀，梯形导向浮阀，V形栅板，导向筛板，NVT减压塔板等，同时在借鉴最新塔板基础研究理论的基础上开发出来的。冷模实验结果表明，在提馏段（汽段）的操作状况下，NS导向提馏塔板与提馏段常用开孔率的F1型浮阀塔板相比，塔板压降降低约13％－50％，泄漏基本没有，雾沫夹带可忽略不计。     

强直性脊柱炎患者中IL-23 R基因rs7517847多态性分析

目的研究IL-23R基因rs7517847位点的单核苷酸多态性与强直性脊柱炎易感性的关系.方法采用PCRRFLP方法对100例强直性脊柱炎患者进行IL-23R基因多态性检测,并与35例健康者对照分析.结果 rs7517847位点各基因型频率和等位基因频率在AS组与对照组之间的分布差异均具有统计学意义(P0.05);并在假设遗传方式下,rs7517847位点的纯合突变GG基因型与(TG+TT)基因型比较,其频率分布差异在AS组与对照组之间也具有统计学意义(P0.05).结论 IL-23R基因rs7517847位点的多态性与吉林地区人群AS易感性有关;携带G等位基因且为GG基因型的个体患AS的危险性增大,这可能是患AS的易感因素之一.     

高精度表面粗糙度非接触式轮廓仪

提出了一种高精度表面粗糙度非接触式轮廓仪.该仪器在6JA干涉显微镜的基础上,采用相移干涉技术、外同步技术和有效算法,以消除背景光强不均匀、人眼瞄准误差、数值孔径等因素的影响,并在条纹对比度很低的情况下也能获得良好的结果.实验结果表明,测量精度比原仪器提高10倍,测量时间仅需3s,垂直分辨率和重复测量精度优于1nm.     

基于散射电场的飞机旋翼多普勒回波模拟

旋翼飞机飞行过程中会产生具有独特多普勒特征的雷达回波,通过对旋翼回波的准确模拟,可为不同类型飞机的精确识别提供重要依据.针对现有散射点叠加算法的假设缺陷,从目标高频电磁散射理论出发,提出了基于散射电场的飞机旋翼回波模拟方法.依据电磁散射场与雷达回波方程间的数学关系,发现目标回波的基带信号与目标后向散射电场的复振幅矢量具...     

1-苯基(甲基)-3-甲基-5-(4,6-二甲基-2-硫代)-2-嘧啶基-4-吡唑甲酸酯类化合物的合成及生物活性

采用相转移催化方法将１－苯基（甲基）－３－甲基－５－（４,６－二甲基－２－硫代）嘧啶基－４－吡唑甲醛氧化成酸,进而与卤代烷反应制得吡唑甲酸酯。所报道的１６个新化合物结构经＾１Ｈ ＮＭＲ、ＩＲ、ＭＳ和元素分析确证,初步的生测结果表明,其中几个化合物对小麦锈病有明显的抑菌活性,个别化合物表现出除草活性。     

氢键对OH伸缩振动频率的影响

计算了水、甲醇和硅烷醇分子单体和相应二聚体的振动频率.分别采用二阶MΦller-Plesset微扰和密度泛函理论方法优化了分子的几何结构,计算了结合能和振动频率.分子的结合能计算考虑了基矢叠加误差校正.通过与实验结果比较评价了两种计算方法,研究了氢键相互作用对分子结构和OH伸缩振动频率的影响.结果表明,较强的氢键相互作用会引起较明显的结构变化,从而导致较大的振动频移.     

基于四元数的空间全方位算法研究

基于误差四元数的概念,研究了四元数扰动对姿态角和四元数转换精度的影响,提出了姿态角和四元数的全方位转换算法.该算法将姿态角和四元数的转换范围由-90°~90°的传统转换空间拓展到-180°~180°空间,使基于全角度算法的姿态控制比非全角度算法有抗扰动的能力,并通过论证和仿真证明了算法的正确性.     

Influence of thermal-decomposition temperatures on structures and properties of V2O5 as cathode materials for lithium ion battery

Submicron spherical V2O5 particles with a uniform size and a lower crystallinity were successfully synthesized by a chemical precipitationthermal decomposition technique using the commercial V2O5 powders as starting material. The crystal structure and grain morphology of samples were characterized by X-ray diffraction(XRD) and scanning electron microscopy(SEM), respectively. Electrochemical testing such as discharge–charge cycling(CD) and cyclic voltammetry(CV) were employed in evaluating their electrochemical properties as cathode materials for lithium ion battery. Results reveal that the crystallinity and crystalline size of V2O5 particles increased when the thermal-decomposition temperature increased from 400 ℃ to 500 ℃, and their adhesiveness was also synchronously increased. This indicate that the thermaldecomposition temperature palyed a significant influence on electrochemical properties of V2O5 cathodes. The V2O5 sample obtained at 400 ℃ delivered not only a high initial discharge capacity of 330 m A h g-1and also the good cycle stability during 50 cycles due to its higher values ofα in crystal structure and better dispersity in grain morphology.