SEM analysis of the changes of carbon layer structure of mesocarbon microbeads heat-treated at different temperatures

Mesocarbon microbeads (MCMB) with narrow size distribution, excellent sphericity and no obvious conglutination have been prepared with a coal tar pitch containing quinoline insolubles (QI) as the raw material. Optical microscopy and scanning electron microscopy (SEM) are used to examine the structure of the MCMB. It has been found that SEM technique shows the structural information of MCMB clearly in the form of micrographs even when the structure of MCMB is complex, while the optical technique is useful for analysis of the regular structure of the mesophase spheres but cannot be effectively used to analyze either the complex structure of the green MCMB or the structures of the further heat-treated ones at different temperatures. According to the characteristics of the carbon layers, the structures of the as-prepared MCMB in the present experiment could be classified as (I) Parallel Layer type structure and (2) Bent Layer type structure with the carbon layers gathering at one or two points in the MCMB. In the experiments, SEM is also utilized to investigate the structures of MCMB that are heat-treated at different temperatures. It has been found that the MCMB without any further heat-treatment show no layered-carbons, while the ones heat-treated at temperaturehigher than 1000℃ exhibit obvious layered carbons across their sections. When increasing the heat-treatment temperature, the carbon layers become thinner and flatter.     

Photocatalytic effect of nature and modified V-bearing rutile

A natural rutile TiO2, which displays photocatalysis on degrading halohydrocarbon pollutants, was reported. The rutile contains up to 1.22% V2O5 (weight percent). Substitutions of Ti by V, as well as Fe, Cu and Zn, result in lattice distortions and defects. Grinding, heating and quenching modify the surface characteristics of ruffle, and consequently increase its photoactivity. It is found that the heating and quenching treatments enhance visible-light absorption of ruffle, especially at 1000 and 1100℃. Ground rutile is effective to degrade tetrachloroethylene and trichloroethylene. While, heating at 1000℃, and quenching at 1000 and 1100℃ distinctly improve degradation rate of samples. The special properties of the natural rutile have particular capabilities in photocatalytic oxidation, especially in degradation of halohydrocarbons.     

重组人尿激酶原氨基末端片段的制备及活性测定

尿激酶原的N-末端135个氨基酸片段包含了表皮生长因子结构域和环柄结构域，参与了尿激酶原和其受体的结合以及尿激酶原诱导的化学趋化活性．利用RT-PCR，从人脐带静脉内皮细胞中克隆ATF基因．将ATF基因插入pET-29a( )中构建表达质粒pET-29a( )／ATF，并转化至大肠杆菌BL21(DE3)中，经IPTG诱导表达及纯化后，从每升培养液中获得15mg rhATF，其纯度超过95％．活性测定结果表明rhATF能够阻断尿激酶原与尿激酶受体的结合，并能抑制尿激酶对纤溶酶原以及纤溶酶对尿激酶原的激活．     

多不饱和脂肪酸对谷氨酸诱导的大鼠海马神经细胞损伤的双重影响

通过测定原代培养的谷氨酸诱导的受损海马神经的细胞存活率,谷胱甘肽过氧化物酶活性,以及谷胱甘肽和一氧化氮的水平,研究多不饱和脂肪酸,如二十二碳六烯酸(docosahexaenoic acid,DHA)、花生四烯酸(arachidonic acid,AA),对大鼠海马神经细胞损伤的影响.海马神经元用神经特异性烯醇化酶进行鉴定.研究发现:(1)DHA 5～50 mg/L能有效的对抗谷氨酸诱导的海马神经细胞的损害作用.表现在细胞存活率、谷胱甘肽过氧化物酶的活性较谷氨酸处理组明显提高,NO水平降低,谷胱甘肽含量与对照组相比变化不大.(2)5 mg/LAA对损伤没有影响,而30和50 mg/LAA对损伤具有加重作用,其细胞存活率、GSH-Px活性、GSH水平较谷氨酸处理组明显降低.结果表明,多不饱和脂肪酸对谷氨酸诱导的神经细胞损伤具有双重作用.     

二十二碳六烯酸乙酯对沙土鼠缺血再灌注脑损伤及水肿作用的研究

研究二十二碳六烯酸乙酯(E-DHA)对沙土鼠缺血再灌注脑损伤及其脑水肿的影响.选用雄性断奶沙土鼠灌胃给予二十二碳六烯酸乙酯(150 mg/(kg·d))或等体积溶媒10周,阻断沙土鼠双侧颈总动脉血流10 min,造成缺血再灌注模型;测定再灌注后脑组织中丙二醛(MDA)水平,还原型谷胱甘肽(GSH)含量,超歧化氧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)和钾、钠-ATP酶(Na ,K -ATPase)活性,脑水含量,钠、钾离子浓度及海马CA1区神经细胞存活百分数.结果表明:E-DHA预处理显著降低缺血再灌注沙土鼠脑组织MDA水平,阻止GSH含量、GSH-PX和Na ,K -ATPase活性下降,而对SOD的活性未表现出明显作用;E-DHA亦调节缺血再灌注沙土鼠脑组织钠、钾离子浓度,减轻缺血再灌注所致的脑水肿和神经细胞死亡.提示E-DHA对脑缺血再灌注脑损伤有显著保护作用,机制可能其与抗氧化作用有关.     

基于相关矩阵对角化和遗传算法的盲源分离法

提出了一种新的基于相关矩阵对角化的代价函数作为衡量输出信号独立性的测度。为了扩大搜索空间,降低各信源之间的互相关性,将代价函数进行了非线性变换。还提出了利用实数编码的遗传算法对代价函数进行最优化搜索,以克服传统梯度搜索方法容易陷入局部收敛的缺点。此方法不仅适用于平稳或非平稳信号,而且还可用于瞬时或卷积混和模型的盲源分离问题。仿真实验表明,该算法具有快速收敛性能和高精确度等优点,能够大大提高分离后的输出信噪比。     

具有紧急消息的航电总线Petri网建模与性能分析

在航空电子综合化系统的设计过程中，总线系统的性能指标与整个航电系统的总体指标有着密切的关系。给出了一套完整的基于确定与随机Petri网的综合航电总线系统的性能评价的模型和算法。通过对总线系统的确定与随机Petri网模型的求解，给出了具有紧急消息的总线系统的两个重要性能指标总线负载、延迟时间，通过对性能曲线的分析，可以为综合航电系统的设计和完善提供重要的理论依据。     

基于多智能体系统的协同交互式进化计算模型

协同交互式进化计算由于缺乏通用模型而限制了其应用范围。针对该问题，结合多智能体系统结构的灵活性和异构特点，提出基于多智能体系统的协同交互式进化计算模型。模型由交互式进化计算单元和协同单元构成，并给出了每个部分的详细功能描述。采用面向智能体的程序设计方法，提取不同功能组件，给出相应组件的语义描述及派生关系。以网络环境下的服装设计系统为例，给出系统结构及组件派生实例。结果表明该模型的合理性，为协同交互式进化计算的广泛应用奠定了基础。     

非线性时间序列建模的混合GARCH方法

在文献[1]的基础上，首次提出混合广义自回归务件异方差（Mixture Generalized Autoregressive Conditional Heteroscedastic Model简记MGARCH）模型；给出并证明了MGARCH模型的一阶平稳性的充分必要条件及二阶平稳性的充分务件；给出该模型参数估计的EM算法：利用BIC定阶准则对MGARCH模型的各成份进行定阶；计算结果表明该模型对金融非线性时间序列中存在的变异率现象具有较强的描述能力，有广阔的应用前景。