Specific binding of antigenic peptides to cell-associated MHC class I molecules

T lymphocytes recognize antigen in the form of peptides that associate with specific alleles of class I or class II major histocompatibility (MHC) molecules. By contrast with the clear MHC allele-specific binding of peptides to purified class II molecules purified solubilized class I molecules either bind relatively poorly or show degenerate specificity. Using photo-affinity labelling, we demonstrate here the specific interaction of peptides with cell-associated MHC class I molecules and show that this involves metabolically active processes.     

Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

再辉效应对T9A钢显微组织和机械性能的影响

研究了T9A钢铅浴淬火时,再辉效应对其显微组织和机械性能的影响.在珠光体转变温度范围内等温时,随着等温温度的降低,引起塑性下降的原因是再辉效应促发颗粒状或短片状珠光体形成;在上贝氏体转变温度区,由于出现再辉效应产生的温度爬升现象,使试样温度升至屈氏体转变温度区从而形成上贝氏体和屈氏体的混合组织,使得强度增高曲线出现平台或稍有下降,且塑性发生下降,同时,还对等温淬火的定义提出了新的看法,绘出了反映实际情况的共析钢C-曲线上的组织形成图。     

关于用奇异函数求解阶梯形梁变形的两个问题

对于n段阶梯形梁,X_o=0,X_1,X_2,……,X_(n-1)为第一、第二、……、第n段梁左截面的坐标,J_1,J_2,.....,J_n为相应段梁的惯性矩。根据,先将梁惯性矩的倒数用阶梯函数表示:其中此时,梁的挠曲线微分方程为其中E为材料的弹性模量;M(X)为梁的弯矩,由奇异函数法求出。依据奇异函数的积分规则,由(3)式可分别得到梁的转角方程和挠度方程:     

《高等数学》学习方法探讨

从高等数学的知识体系及其学习的特点与基本要求入手,论述了学习高等数学的基本方法,即阐述了如何接收信息、如何消化材料和所学知识如何运用等问题。     

虚拟现实与电子商务的结合探讨

提出了一种虚拟现实应用于电子商务的新方法,这种方法能对目前电子商务普遍性问题进行解决,但虚拟技术正处在发展阶段,本方法还有很大的改进空间.     

浅谈吕梁三泉焦化工业园区区域定位研究

文章以科学发展观为指导,以做大、做强、做优特色园区为目标,以实现高效、稳健、科学发展为前提,分析并提出了吕梁三泉焦化工业园区的发展定位.     

对我国住房价格形成机制的思考

通过分析中国住房价格的形成机制,揭示了我国房价虚高的深层原因,并指出了这种现象存在的必然性和未来发展趋势.