谷胱甘肽-铜极谱络合吸附波的研究

在铜离子存在下,谷胱甘肽(GSH)在0.2mol/LNaAc-HAc(pH4.7)缓冲溶液中,与铜离子反应得到谷胱甘肽(GSH)与一价铜离子络合物,在单扫示波极谱上产生一灵敏的络合吸附波,峰电位在-0.59V处(vs.SCE),导数波高与谷胱甘肽浓度在4.0×10-8mol/L～1.0×10-5mol/L范围内分段成线性关系,检测限为1.0×10-8mol/L,相对标准偏差3.3%。研究了极谱波的性质,测得络合物组成为GSH:Cu(Ⅰ)=1:1,表观稳定常数为5.1×1019。     

Research on the integration of Web_-CNC and EIS

1.INTRODUCTION Withthedevelopmentofcomputerandnetwork,the competitionofmarketbecomingincreasinglyserious,andtherequirementofcustomerschangingfaster,enterprisecannotadaptitselftothemarketdemand withthetraditionalmanagingmode.Inordertosuc ceedinmarket,enterprisemustreacttothemarket agilely,andmustbuildnetworkedmanufacturing modebasedontheT(time),Q(quality),C(cost),S(service),E(environment),andK(knowl edge)[1,2].Atpresent,enterprisestrengthenstheagility throughimplementingERP(enterprisere…     

中国奥林匹克教育之刍议

本文采用文献资料和逻辑分析等方法阐释了奥林匹克教育渊源和内涵、奥林匹克教育的内容和对象,分析了中国奥林匹克教育的现状和存在的问题,并试图以2008年北京奥运会为契机,为推动奥林匹克教育在中国广泛深入地开展和普及提出建议。     

李雅普诺夫泛函与具时滞的非线性系统的解的渐近性

得到了具时滞的非线性微分方程系统的渐近性的一些结果，所用的方法是构造李雅普诺夫泛函数持久性泛函。得到的结果将不依赖于时滞的大小。     

试论高校扩招形势下大学生就业压力的缓解对策

高校扩招是世界各国高等教育发展的必然趋势,它为经济发展提供了丰富的人才储备,增强了经济和社会发展的后劲,但高校扩招使大学毕业生数量在短期内增长过快,造成空前的就业压力,这是不可避免的。要缓解大学生就业压力,应从政府采取政策、措施宏观调控;高校保证教育质量、加强就业指导、就业推荐;全社会开辟多种就业渠道,形成就业市场调节就业;大学生积极自主就业等多方面相结合,多管齐下,形成合力,努力保持大学生就业形势的基本稳定。     

Detection of Grass Carp Hemorrhage Virus （GCHV） from Vietnam and Comparison with GCHV Strain from China

Grass carp plays an important role in small-scale aquaculture in Vietnam. However, a severe disease, known in Vietnam as “Red Spot Disease“, is causing significant economic loss in grass carp aquaculture. In this study, the tissue samples isolated from the grass carp with Red Spot Disease in Vietnam are investigated and eomparied with the control GCHV isolated in China by experimental infection, culture cell infection, serological cross reactivity, and RT-PCR amplifica-tion. Infected grass carp exhibits hemorrhage symptoms about 5 days after experimental injection with GCHV-V (Vietnam) strain. The symptoms and lethality induced by the GCHV-V strain are identical to that induced by the Chinese GCHV-9014 strain. The Chinese GCHV-873 strain in-duces typical cytopathogenic effects in 4 cell lines, such as CIK, CAB, FHM and GCO, from the6 fish cell lines examined. No cytopathogenic effects are observed in all the 6 examined cell lines,including CAB, FHM, CIK, EPC, CCO and G(X), infected by the GCHV-V strain and GCHV-9014 strain. Immunodiffusion assays demonstrate an obvious cross-reactivity among three GCHVstrains. Precipitin lines are clearly observed not only between the anti-GCHV-873 serum and thetwo strains GCHV-873 and GCHV-9014, but also between the anti-GCHV-873 serum and the GCHV-V strain. GCHV can be detected by immunodiffusion assays after three generations of blind propagations in the cell lines inoculated by GCHV-V strain. This implicates that GCHV-Vviruses have been replicated and amplified despite there being no cytopathogenic eifects observed in these examined cell lines. Three genome segments of GCHV, including S8, S9 and S10, are amplified by three sets of PCR primers designed according to the segment sequences published re-cently. The Q8fp and Q8rp primer set specific for genome segment S8 amplifies a 955 bp frag-ment from the extracted sample of diseased fish with Red Spot Disease, and the fragment size is i-dentical to that amplified by the same primer set from control GCHV-873 strain. Simultaneously,the Q9fp and Q9rp primer set specific for genome segment S9 generates a same 635 bp product,and the Q10fp and Q10rp primer set specific for genome segment S10 produces a same 697 bp fragment from both template samples of diseased fish with Red Spot Disease and control GCHV-873 strain. The RT-PCR amplification and corresponding size comparison data indicate that the three GCHV-V genome segments extracted from the diseased grass carp with Red Spot Disease in Vietnam should be identical to that in control GCHV-873 strain from China. The data confirm that the causative agent of grass carp Red Spot Disease in Vietnam is a virus, and the virus is closely similar to GCHV strain in China.     

胶粘剂实验教学改革与实践

胶粘剂实验教学目前仍是继承原有单一的、传统的验证性实验,这对创新意识和创新能力的跨世纪人才培养,无疑是不利的。针对这一现状,从实验教学内容、教学方法等入手,对胶粘剂实验教学进行了必要的改革与实践。     

循环水冷系统温控电阻的实验测定

循环水冷系统温控电路的热敏电阻失效了,该电阻无标志,不知其阻值。通过实验测试,确定了它的阻值及温度系数,从而修复了系统。     

基于任务的导学型网络学习共同体的构建

从学习共同体的概念出发．对网络学习共同体中的构成要素进行分析．设计了基于目标的导学型网络学习共同体的模型，说明了进行网络学习的过程，列举了通过网络共同体进行网络学习的常见方式，并强调了助学者在网络学习中导学的重要性。     

Study on read-only optical disk with Sb mask super-resolution

In this note, by using Sb to replace the Al reflective layer in the conventional optical disk, the recording pits with a diameter 380 nm and a depth 50 nm are read out in the device, whose laser wavelength l is 632.8 nm and numerical aperture (NA) is 0.40. In the course of reproduction, the readout laser power is 4 mW and the rotation velocity of the disk is 5 m/s. The optimum Sb film thickness is 28-30 nm and the CNR is 38-40 dB.