Cinobufacini-induced HeLa cell apoptosis enhanced by curcumin

When used in combination with certain chemotherapies, curcumin has been shown to increase apoptosis in several cancer cell lines. Here, we report the combined effects of curcumin and cinobufacini on human cervical carcinoma cells. The aim of this study was to examine whether curcumin could enhance apoptosis induced by cinobufacini. 3-(4,5-Dimethylthiazol-2-y1)-2,5- diphenytetrazolium bromide (MTT) assays revealed that the growth and proliferation of HeLa cells could be inhibited by 75% after a combined treatment of 25 μg/mL cinobufacini and 8 μg/mL curcumin. The combined treatment is 3 times more effective than treatment with 25 μg/mL cinobufacini alone. Annexin V-FITC/PI staining, morphological changes and immunofluorescence verified a significant enhancement in cinobufacini-induced apoptosis when cells were also exposed to curcumin. The data showed that the proportion of early apoptotic cells significantly increased from 15.43% in cells treated only with 25 μg/mL cinobufacini to 49.2% in cells treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. Moreover, compared with treatment of only 25 μg/mL cinobufacini, ROS production increased 1.7-fold, the intracellular free Ca 2+ concentration increased 1.5-fold, and the mitochon- drial membrane potential decreased by 20% in the combined treatment. Simultaneously, the atomic force microscopy (AFM) re- sults suggest that cells treated with a combination of cinobufacini and curcumin varied significantly in shape and ultrastructure. Collapsed cells with leaking cytoplasm, blebbing pores and emerging apoptotic bodies were prevalent. The nanoparticle size increased from 70 nm when the cells were treated with 25 μg/mL cinobufacini to 190 nm when the cells were treated with 25 μg/mL cinobufacini and 8 μg/mL curcumin. The size increase resulted in the cell membrane becoming considerably rough. These results can improve our understanding of combination treatments. Specifically, the combination of cinobufacini and curcumin may potentially find use as a novel cervical carcinoma treatment. Additionally, AFM is a powerful tool that can be used to explore cellular morphologies and ultrastructures.     

基于数据挖掘的网络业务流分析方法

为了从业务角度对网络的性能进行评价和优化,提出了一种新的网络业务分析方法——具有时态路径约束的关联规则挖掘分析方法.该方法以网络业务为分析粒度,以与网络业务流相关的时态属性和路径属性为约束条件,对已经积累的反映网络状况的海量历史数据进行挖掘分析.在进行关联规则挖掘时,利用频繁数据项集的性质,通过引入事务标号,在求出候选频繁项集的同时也求出其支持度,避免了为求支持度而进行的扫描数据库运算,极大提高了挖掘的效率和速度.实验结果表明,进行挖掘分析的数据量越大,该方法的性能和效率就越好.     

Shape demagnetization effect on layered magnetoelectric composites

Magnetoelectric （ME） voltage coefficient of plate layered magnetostricUve-piezoelectric composites was always considered independent of the in-plane size due to the oversimplification in ME theoretical models. Here we report that the ME voltage coefficient depends on the in-plane size owing to shape demagnetization effect on magnetostriction. Both theoretical analysis and experimental results indicate that ME voltage coefficient of layered ME composites changes with the variation in in-plane sizes （length and width）. ME coefficient increases with a rise in in-plane sizes, and different aspect ratio also results in different ME coefficients. Proper design of in-plane shape will greatly promote the development of ME devices.     

一种用于异常检测的网络流量抽样方法

为了减小抽样数据对网络异常检测的影响,提出了一种新的可变抽样率的网络流量抽样方法.通过利用哈希模式匹配算法,将到达的数据报文按流标识分类并记录下该报文在流中的位置,然后根据报文所属流的位置顺序减函数来设置不同的报文抽样概率.实验结果表明,所提方法增加了短流报文的抽样概率,解决了由于随机报文抽样方法偏向于长流抽样而导致的网络异常丢弃的问题,从而提高了异常检测的正确性.     

我国体育赛事电视转播权经营开发的影响因素探析

从体育、广电小系统到整个社会大系统,审视了影响国内体育赛事电视转播权经营开发的主、客观因素,科学探寻了问题背后的原因和症结,提出了有过渡性特征,有中国特色的体育赛事电视转播权经营开发的对策建议,以期为规范和推进国内赛事电视转播权的有序开发提供参考。     

图的顶点划分与图的上可嵌入性

图G的顶点W-划分是指G的一个顶点划分{V1,V2,…,Vs),其中G[Vi]有生成子图轮W[V1](1≤i≤s).结合图的顶点W-划分以及顶点度条件,得到了一类新的上可嵌入图类,推广了已有相关结果.     

Anatase TiO2 single crystals with a large percentage of reactive facets

Owing to their scientific and technological importance, inorganic single crystals with highly reactive surfaces have long been studied. Unfortunately, surfaces with high reactivity usually diminish rapidly during the crystal growth process as a result of the minimization of surface energy. A typical example is titanium dioxide (TiO2), which has promising energy and environmental applications. Most available anatase TiO(2) crystals are dominated by the thermodynamically stable {101} facets (more than 94 per cent, according to the Wulff construction), rather than the much more reactive {001} facets. Here we demonstrate that for fluorine-terminated surfaces this relative stability is reversed: {001} is energetically preferable to {101}. We explored this effect systematically for a range of non-metallic adsorbate atoms by first-principle quantum chemical calculations. On the basis of theoretical predictions, we have synthesized uniform anatase TiO(2) single crystals with a high percentage (47 per cent) of {001} facets using hydrofluoric acid as a morphology controlling agent. Moreover, the fluorated surface of anatase single crystals can easily be cleaned using heat treatment to render a fluorine-free surface without altering the crystal structure and morphology.     

基于高斯曲率和变指数扩散系数的图像去噪模型

由于TV模型在去噪的过程中容易模糊小梯度边缘并产生虚假边缘,本文提出一种联合高斯曲率和变指数扩散系数的图像消噪模型.试验表明,该模型能更好地保留小梯度特征,减少虚假的边缘的产生.     

基于酰腙和酚羟基的阴离子受体合成及阴离子比色识别性能研究

设计合成了一种基于酚羟基的酰腙类受体分子,利用紫外-可见吸收光谱考察了其与F-,CI-,Br-,I-,CH3COO-,H2PO4-,HSO4-,ClO4-的相互作用.结果表明,该类受体分子在DMSO溶液中既能识别中性的Br-和I-,也能比色识别碱性较强的F-,CH3COO-和H2PO4-,其中在加入Br-,I-时UV-VIS光谱产生蓝移,而在加入F-,CH3COO-,H2PO4-时UV-VIS光谱产生红移.     

付费体育频道运营的SWOT分析

运用SWOT分析方法,对国内付费体育频道生存环境的内在优势和劣势,外部机遇和威胁进行了剖析,认为国内付费体育频道运营还处于艰难的市场培育期,要注重发挥优势,克服不足,利用机会,化解威胁,推动付费体育频道科学、合理、健康发展。