耐高浓度甘油的1,3-丙二醇生产菌的诱变选育

利用紫外线对克雷伯杆菌进行诱变,筛选出耐高浓度甘油且1,3-丙二醇(1,3-PD)产量较高的突变株KpⅥ.实验结果表明,在距离34 cm,功率30 W的紫外灯垂直照射下,最佳紫外诱变时间为6 min,此时,致死率为90.9%.克雷伯杆菌经过紫外诱变后,菌落明显增大,是原始菌株的2~4倍;变异菌株KpⅥ经过6代遗传稳定性考察,甘油消耗率和1,3-PD产量稳定,且保持了较高水平.在甘油质量浓度为90 g.L-1的条件下,变异菌株KpⅥ的甘油消耗率为98.3%,甘油转化率为50.4%,1,3-PD产量为44.81 g.L-1,生产能力达到0.75 g.(L.h)-1.     

Blockage of the NLRP3 inflammasome by MCC950 improves anti-tumor immune responses in head and neck squamous cell carcinoma

The NLRP3 inflammasome is a critical innate immune pathway responsible for producing active interleukin (IL)-1β, which is associated with tumor development and immunity. However, the mechanisms regulating the inflammatory microenvironment, tumorigenesis and tumor immunity are unclear. Herein, we show that the NLRP3 inflammasome was over-expressed in human HNSCC tissues and that the IL-1β concentration was increased in the peripheral blood of HNSCC patients. Additionally, elevated NLRP3 inflammasome levels were detected in tumor tissues of Tgfbr1/Pten 2cKO HNSCC mice, and elevated IL-1β levels were detected in the peripheral blood serum, spleen, draining lymph nodes and tumor tissues. Blocking NLRP3 inflammasome activation using MCC950 remarkably reduced IL-1β production in an HNSCC mouse model and reduced the numbers of myeloid-derived suppressor cells (MDSCs), regulatory T cells (Tregs) and tumor-associated macrophages (TAMs). Moreover, inhibiting NLRP3 inflammasome activation increased the numbers of CD4⁺ and CD8⁺ T cells in HNSCC mice. Notably, the numbers of exhausted PD-1⁺ and Tim3⁺ T cells were significantly reduced. A human HNSCC tissue microarray showed that NLRP3 inflammasome expression was correlated with the expression of CD8 and CD4, the Treg marker Foxp3, the MDSC markers CD11b and CD33, and the TAM markers CD68 and CD163, PD-1 and Tim3. Overall, our results demonstrate that the NLRP3 inflammasome/IL-1β pathway promotes tumorigenesis in HNSCC and inactivation of this pathway delays tumor growth, accompanied by decreased immunosuppressive cell accumulation and an increased number of effector T cells. Thus, inhibition of the tumor microenvironment through the NLRP3 inflammasome/IL-1β pathway may provide a novel approach for HNSCC therapy.     

Seven new species of the genus Pseudostegana (Diptera: Drosophilidae) from South-west China,with DNA barcoding information

Seven new species of the genus Pseudostegana are discovered from South China: P. latiapicula sp. nov., P. nannanae sp. nov., P. bingxueae sp. nov., P. husaensis sp. nov., P. linyongi sp. nov., P. montipagana sp. nov., and P. sigangli sp. nov.; the first two new species belong to the P. latiparma species group, and the rest belong to the P. zonaria species group. The DNA sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene with BOLD Process ID and GenBank accession numbers are provided for the seven new species and 10 known species from southern China: P. acutifoliolata Li, Gao and Chen, 2010; P. angustifasciata Chen and Wang, 2005, P. bifasciata Chen, Toda and Wang, 2005, P. bilobata Li, Gao and Chen, 2010; P. insularis Li, Gao and Chen, 2010; P. latifasciata Chen, Toda and Wang, 2005, P. minutipalpata; P. nitidifrons Chen and Wang, 2005, P. pallidemaculata Chen and Wang, 2005 and P. silvana. Intra- and interspecific pairwise Kimura’s two-parameter (K2P) distances among these species are summarised. www.zoobank.org/urn:lsid:zoobank.org:pub:C01D7920-61AD-4651-8861-9D76C3CD86F6     

Molecular and Cellular Basis of Regeneration and Tissue Repair

The Xenopus tadpole is a favourable organism for regeneration research because it is suitable for a wide range of micromanipulative procedures and for a wide range of transgenic methods. Combination of these techniques enables genes to be activated or inhibited at specific times and in specific tissue types to a much higher degree than in any other organism capable of regeneration. Regenerating systems include the tail, the limb buds and the lens. The study of tail regeneration has shown that each tissue type supplies the cells for its own replacement: there is no detectable de-differentiation or metaplasia. Signalling systems needed for regeneration include the BMP and Notch signalling pathways, and perhaps also the Wnt and FGF pathways. The limb buds will regenerate completely at early stages, but not once they are fully differentiated. This provides a good opportunity to study the loss of regenerative ability using transgenic methods.     

Galanin – 25 years with a multitalented neuropeptide

Galanin, a neuropeptide widely expressed in the central and peripheral nervous systems and in the endocrine system, has been shown to regulate numerous physiological and pathological processes through interactions with three G-protein-coupled receptors, GalR1 through GalR3. Over the past decade, some of the receptor subtype-specific effects have been elucidated through pharmacological studies using subtype selective ligands, as well as through molecular approaches involving knockout animals. In the present review, we summarize the current data which constitute the basis of targeting GalR1, GalR2 and GalR3 for the treatment of various human diseases and pathological conditions, including seizure, Alzheimer's disease, mood disorders, anxiety, alcohol intake in addiction, metabolic diseases, pain and solid tumors.     

基于TACACS协议的身份认证技术分析与研究

随着网络经济时代的到来,网络将会成为一个无处不在、无所不用的工具。网络安全问题越来越成为人们关注的焦点。本文针对网络安全中的身份认证技术,探讨了TACACS协议,并结合实例分析和研究了认证系统的建立。     

企业联盟与企业集团的组建及运行成本分析比较

将企业联盟和集团的组建过程分解为四个阶段 ,通过定性分析建立其组建成本模型 ,运用Kuhn-Tucker条件 ,得到各组建阶段的最优时间分配 ,然后对二者组建成本的相对重要程度进行了比较 .再次 ,运用排队论的思想 ,比较了汽业联盟和企业集团.     

基于神经网络的Lyapunov指数谱的计算

利用 BP神经网络的非线性函数逼近能力 ,由实验观察数据列计算系统的 Lyapunov指数谱 .实例计算表明 ,此种方法精度较高且计算量较小 ,有重要的实际意义.     

风机振动分析与故障诊断

应用振动分析方法、数据采集技术及诊断理论对某乙烯厂Ｅ－ＧＢ１０９风机进行了现场振动测量、信号的采集与分析诊断,找出了风机振动的原因。     

轨检车检测数据处理分析软件关键业务研究

从业务模型的构建和关键业务分析两个方面对检测数据里程转换和波形图对比两项关键业务进行研究,构建了检测数据里程转换模型,进行了波形图绘制和波形图联动方法的研究,并对检测数据里程转换和波形图对比这两项关键业务的业务需求及业务流程图进行了分析与绘制,最终列出了两项关键业务的功能实现情况。研究轨检车检测数据处理分析软件的关键业务,从而更高效地运用软件分析与处理轨检车数据,为保证地铁线路的安全运营奠定基础。