结构动力分析一种简易的直接积分法

本文利用方块脉冲函数优良的运算性质,提出一种结构动力分析简易的直接积分法——方块脉冲函数法,具有无条件稳定的积分格式,并表明方法的可行和有效.     

Structure of the detoxification catalyst mercuric ion reductase from Bacillus sp. strain RC607

Several hundred million tons of toxic mercurials are dispersed in the biosphere. Microbes can detoxify organo-mercurials and mercury salts through sequential action of two enzymes, organomercury lyase and mercuric ion reductase (MerA). The latter, a homodimer with homology to the FAD-dependent disulphide oxidoreductases, catalyses the reaction NADPH + Hg(II)----NADP+ + H+ + Hg(0), one of the very rare enzymic reactions with metal substrates. Human glutathione reductase serves as a reference molecule for FAD-dependent disulphide reductases and between its primary structure and that of MerA from Tn501 (Pseudomonas), Tn21 (Shigella), p1258 (Staphylococcus) and Bacillus, 25-30% of the residues have been conserved. All MerAs have a C-terminal extension about 15 residues long but have very varied N termini. Although the enzyme from Streptomyces lividans has no addition, from Pseudomonas aeruginosa Tn501 and Bacillus sp. strain RC607 it has one and two copies respectively of a domain of 80-85 residues, highly homologous to MerP, the periplasmic component of proteins encoded by the mer operon. These domains can be proteolytically cleaved off without changing the catalytic efficiency. We report here the crystal structure of MerA from the Gram-positive bacterium Bacillus sp. strain RC607. Analysis of its complexes with nicotinamide dinucleotide substrates and the inhibitor Cd(II) reveals how limited structural changes enable an enzyme to accept as substrate what used to be a dangerous inhibitor. Knowledge of the mode of mercury ligation is a prerequisite for understanding this unique detoxification mechanism.     

立式割台后转向二次切割装置试验研究

本文通过立式割台后转向二次切割的试验研究,提出了作物稳定转向和二次切刀合理的工作参数,为二次切割型立式割台小型联合收割机提供了理论与试验依据.     

广西农业专家系统的建立和应用

广西农业专家系统的开发建设主要集中在基础数据库建设、知识库建设、人机界面的设计和改进方面。该系统建有农业气象数据库、土壤数据库、品种数据库和病虫害数据库以及龙眼专家系统、荔枝专家系统、果专家系统和甘蔗专家系统。这些农业专家系统在广西30个县 (市 )进行示范种植 (示范面积 6 .5 0× 10 4hm2 ) ,获得了增产增收、降低生产成本的效果。     

材料试验机的升级改造及其在教学中的应用

利用现有设备，通过添加数据采集系统和计算机分析处理系统，使试验机得到升级改造，基本达到自动测试的要求。应用于高职学生的实验教学中，对扩大学生的知识面，提高动手能力有较好的效果。