Thyroid hormone receptor-mediated regulation of the methionine adenosyltransferase 1 gene is associated with cell invasion in hepatoma cell lines

The thyroid hormone T₃ regulates differentiation, growth, and development. We demonstrated that methionine adenosyltransferase 1A (MAT1A) was positively regulated by T₃ identified by cDNA microarray previously. The expression of the MAT1A was upregulated by T₃ in hepatoma cell lines overexpressing thyroid hormone receptors (TRs). Additionally, these findings indicate that MAT1A may be regulated by CCAAT/enhancer binding protein (C/EBP). The critical role of the C/EBP binding sites was confirmed by the reporter or chromatin immuno-precipitation (ChIP) assay. In addition, C/EBP was upregulated in hepatoma cells after T₃ treatment and ectopic expression of MAT1A inhibited cell migration and invasion in J7 hepatoma cells. Conversely, knockdown of MAT1A expression increased cell migration. Together, these findings suggest that the expression of the MAT1A gene is mediated by C/EBP and is indirectly upregulated by T₃. Finally, TR was downregulated in a small subset of hepatocellular carcinoma cells concomitantly reduced the expression of C/EBPα and MAT1A.     

具有多时变时滞的控制系统的绝对稳定性

对具有多个时变时滞的Lurie型控制系统的绝对稳定性进行讨论。假定时变时滞有界且其导数有界，利用李雅普诺夫方法给出了系统绝对稳定的若干充分条件，这些条件利用了时变时滞的界或其导数的界。     

羊毛低温染色的研究

本文采用乙醇胺予处理(简称MEA)及染浴助剂相结合的方法,对媒介染料羊毛染色进行了探讨。文中分析了MEA予处理羊毛对染色的影响;讨论了低温促染剂GF在媒介染料染色中的作用机理;确定了在85℃条件下进行低湿染色的工艺。该工艺与常规染色相比,提高了上染率,减少了纤维损伤,同时改善了纤维性能,提高了折皱回复角。     

纳豆激酶原基因的克隆及表达

从纳豆菌中克隆具有溶血栓性质的纳豆激酶原的基因，与质粒PBV220连接，转化大肠杆菌E.coli DH5α。挑取阳性克隆用EcoR I/BamH I双酶切鉴定得到一条约1000bp的条带。阳性克隆经温度诱导表达，用SDS－PAGE电泳鉴定，在38kD处有一条明显的带，占菌体蛋白的20％左右，同时还表明该蛋白以包涵体的形式蛋白分子量为存在。包涵体经收集、蛋白变性及复性后显示溶血栓活性。     

AC-Link数字音频VHDL编/解码的FPGA设计

提出一种利用FPGA实现AC—Link数字音频处理的设计方案．AC—Link音频编解码系统以VHDL模块进行设计，经过波形仿真和结果验证后，将程序下载到FPGA中实现．这种设计方法可以缩短设计周期，提高设计的可靠性和效率．     

猕猴桃微量硒的测定和施加硒肥的比较研究

用DAN荧光法测定了施硒肥和未施硒肥的猕猴桃花、叶、果中硒含量,结果表明：在相同的土壤营养等条件下,施加硒肥的猕猴桃中牺含量明显比未施硒肥的猕猴桃中硒含量高,且硒在猕猴桃植株中各部位的分布为：叶＞花＞果,提示场肥叶面喷施将比上施效果显著。     

分布式热网参数计量采集系统

介绍了适用于城市集中供热热网参数计量采集管理系统,系统为上、下二级分布式结构。系统下位机包括热网首端计量仪,以及６０个通用供热网点参数计量终端机,下位机系统主要完成整个热参数实时检测及供热量计算。系统上位机为由４８６兼容机,彩色显示器,打印机及缓图仪组成的数据采集管理系统。系统具有全网通讯和数据管理功能,以及计算数据存盘（软盘）等,系统数据通讯采用串行异步通讯,有线传输,最远传输距离可达１５ｋｍ。     

Quantitative analysis of 3-dimensional root architecture based on image reconstruction and its application to research on phosphorus uptake in soybean

The root system is an important organ for plants not only because it absorbs water and nutrients, but also because it provides anchorage against uprooting forces[1,2]. Root architecture is the spatial configuration of the root system in the growth medium[…     

基于数字化共轭曲面理论的数字齿面共轭求解

基于数字化共轭曲面理论和方法,根据啮合传动的规律,应用自行研制开发的共轭曲面求解软件Conjugater1．0．对直齿面和鼓形齿面的数字化共轭曲面求解分别进行了研究．可为各种齿轮的数字化啮合分析、模拟仿真以及数字加工技术提供理论和技术分析参考．     

褪黑素对IP诱导Tau过度磷酸化的保护作用

通过对大鼠海马双侧注射异丙肾上腺素(IP)或同时注射褪黑素(0．1μg,1μg),利用免疫组化、免疫印迹和Morris水迷宫等指标,在整体上观察褪黑素对IP诱导的骨架蛋白Tau过度磷酸化和动物保留记忆损伤的保护作用．结果显示0．1μg和1μg剂量的褪黑素能明显降低IP引起的海马骨架蛋白Tau Ser396／404位点的过度磷酸化;1μg褪黑素可明显改善IP引起的动物保留记忆损伤,提示褪黑素对大鼠学习记忆和海马骨架蛋白Tau有一定的保护作用．