念慈庵川贝枇杷膏止咳化痰平喘药效学的研究

研究念慈庵川贝枇杷膏的止咳、化痰和平喘药效作用。方法:采用小鼠氨水引咳法观察止咳作用;用小鼠酚红排泌法观察化痰作用;用喷雾致喘法观察平喘作用。结果:念慈庵川贝枇杷膏能明显延长引起半数小鼠咳嗽的氨水喷雾时间(EDT50),能促进小鼠气管酚红的排泌,对组胺和氯化乙酰胆碱混合液引起的豚鼠哮喘具有抑制作用。结论:念慈庵川贝枇杷膏具有明显止咳、化痰和平喘作用。     

河岸带的边缘结构特征与边缘效应

河岸带是一个典型的边缘区,根据河岸带纵向空间的镶嵌性、横向空间的过渡性、垂向空间的成层性以及时间分布的动态性等边缘结构特征,提出了河岸带边缘效应的概念.指出河岸带边缘效应是河岸带在结构、状态、行为和功能等方面具有明显的异质性和独特性,这种边缘效具有过渡性、动态变化涨落、易超承载运行等特点,并具体表现为地理边缘效应、生态边缘效应、缓冲边缘效应以及社会边缘效应等.     

高温烃降解菌在含油污水生化处理中的应用

为强化油田污水的生化处理效果,从胜利油田采出液中筛选出3株高温烃降解菌,分别编号为JQ-1、JQ-2、JQ-3,初步鉴定JQ-1、JQ-2为芽孢杆菌属,JQ-3为不动杆菌属,其原油降解率分别为48%、55%、46%。将3株菌混合,其对原油的降解效果好于单株菌,其降解率可达到70%以上,混合菌降解原油的适宜条件分别是温度为45~55℃、pH值为5.5~6.5、矿化度12000~18000mg/L。在室内模拟现场条件进行污水生化处理试验,结果表明：含油量为40mg/L左右的油田污水经过8h处理后含油量在2mg/L以下,同时能有效的抑制硫酸盐还原菌的生长。该混合菌在一定程度上提高了含油污水生化处理的可行性,为油田污水治理提供了理论基础。     

中国东北地区与白俄罗斯全新世成炭期对比及成因分析

通过两地成炭期对比，分析其成炭气候差异和原因，揭示出影响两地成炭作用的气候机制，进而探讨了北方湿润气候带的全新世变迁。     

A carbon－and oxygen－isotoperecord of recent environ－mental change from Qinghai Lake, NE Tibetan Plateau

A c. 300-year oxygen and carbon isotope record derived from fine-grained and ostracod carbonate from Qinghai Lake testifies to dramatic interannual to illterde-cadal linmologicai change. Fine-grained carbonates, whichare mainly authigenic, are likely to have formed in the epillmnion of the lake and their isotopic composition reflects the summer temperature and, more importantly, the isotopic composition of the near-surface waters, which is mainly afunction of evapor ative concentration. Ostracod shells aresecreted in the benthos of the lake, and their isotopic compo-sition reflects summer bottom-water conditions, together with fractionation effects, which may differ between species.Differences between contemporaneous values from authi-genic carbonates and ostracod shells may provide an indica-tion of stratification within the lake and variations in effec-tive precip itation over the northeast part of the Tibetan Pla-tcau over the past 300 years. A period of moderate evapora-tive concentration, from about 300 to 100 yr BP, was inter-rupted by a marked wet phase from-100 to 40 vr BP. which was in turn followed by a return to drier conditions in the most recent part of the record. The increase in δ^18O values in the latter part of the record accords well with instrumental records of lake-level lowering and salinity increase since about 1955 AD.     

单磁泡静态泡径稳定区域的确定

本文计算了在饱和磁化的磁膜中形成单磁泡时系统能量的增量,用理论方法研究了单磁泡稳定存在的条件。从d_0/h和d_2/h与h/l函数关系的图解分析可见:磁膜厚h略增加时,泡径稳定区域变化不大,所以实际设计磁泡器件时为了增大磁泡信号的检出感度,建议最佳泡径d和最佳磁膜厚h都取8l,l为材料的特性长度。     

Enhancement of transfection efficiency for HeLa cells via incorporating arsinine moiety into chitosan

Arginine-rich peptides have attracted considerable attention due to their distinct internalization mechanism. It was reported that arginine and guanidino moieties were able to translocate through cell membranes and played a critical role in the process of membrane permeation. In this work, arginine was conjugated to the backbone of chitosan to form a novel chitosan derivative, arginine modified chitosan （Arg-CS）. Arg-CS/DNA complexes were prepared according to the method of coacervation process. The physicochemical properties of Arg-CS and Arg-CS/DNA complexes were characterized and the transfection activity and efficiency mediated by Arg-CS/DNA complexes were investigated taking HeLa cells as target cells. Arg-CS was characterized by FTIR and ^13C NMR. Arg-CS/DNA polyelectrolyte complexes were investigated by agarose gel retardation, dynamic light scattering （DLS） and atomic force microscopy （AFM）. The results revealed that the Arg-CS/DNA complexes started to form at N/P ratio of 2：1, and the size of particles varied from 100 to 180 nm. The cytotoxicity of Arg-CS and their complexes with plasmid DNA were determined by MTT assay for HeLa cells, and the results suggested that Arg-CS/DNA complexes were slightly less toxic than Arg-CS. Moreover, the derivative alone and their complexes showed significantly lower toxicity than PEI and PEI/DNA complexes, respectively. Taking HeLa cells as target cells and using pGL3-control as reporter gene, the luciferase expression mediated by Arg-CS was greatly enhanced to about 100 folds compared with the luciferase expression mediated by chitosan at different pH media. These results suggest that Arg-CS is a promising candidate as a safe and efficient vector for gene delivery and transfection.     

不同乳化体系下甲基丙烯酸三氟乙氧基乙酯细乳液聚合

分别以十六烷基三甲基溴化铵(CTAB)、十二烷基硫酸钠(SDS)、烷基酚聚氧乙烯醚(OP-10)和全氟辛酸铵(PFOA)为乳化剂,十六烷(HD)为助乳化剂,研究不同乳化体系对甲基丙烯酸三氟乙氧基乙酯(TFEOEMA)细乳液聚合的影响.结果表明,CTAB的乳化稳定作用最好、SDS次之、PFOA较差,OP-10不能用作TF...