全文获取类型
收费全文 | 44937篇 |
免费 | 233篇 |
国内免费 | 470篇 |
专业分类
系统科学 | 625篇 |
丛书文集 | 889篇 |
教育与普及 | 130篇 |
理论与方法论 | 205篇 |
现状及发展 | 18899篇 |
研究方法 | 1716篇 |
综合类 | 22292篇 |
自然研究 | 884篇 |
出版年
2014年 | 332篇 |
2013年 | 643篇 |
2012年 | 942篇 |
2011年 | 1822篇 |
2010年 | 624篇 |
2009年 | 538篇 |
2008年 | 1063篇 |
2007年 | 1206篇 |
2006年 | 1181篇 |
2005年 | 1047篇 |
2004年 | 924篇 |
2003年 | 793篇 |
2002年 | 821篇 |
2001年 | 1288篇 |
2000年 | 1274篇 |
1999年 | 879篇 |
1992年 | 775篇 |
1991年 | 596篇 |
1990年 | 647篇 |
1989年 | 615篇 |
1988年 | 593篇 |
1987年 | 692篇 |
1986年 | 612篇 |
1985年 | 757篇 |
1984年 | 621篇 |
1983年 | 475篇 |
1982年 | 475篇 |
1981年 | 487篇 |
1980年 | 603篇 |
1979年 | 1217篇 |
1978年 | 1010篇 |
1977年 | 1027篇 |
1976年 | 809篇 |
1975年 | 870篇 |
1974年 | 1231篇 |
1973年 | 1090篇 |
1972年 | 1078篇 |
1971年 | 1203篇 |
1970年 | 1470篇 |
1969年 | 1195篇 |
1968年 | 1167篇 |
1967年 | 1183篇 |
1966年 | 1017篇 |
1965年 | 730篇 |
1959年 | 388篇 |
1958年 | 639篇 |
1957年 | 443篇 |
1956年 | 373篇 |
1955年 | 370篇 |
1954年 | 357篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
82.
J E Landegent N Jansen in de Wal G J van Ommen F Baas J J de Vijlder P van Duijn M Van der Ploeg 《Nature》1985,317(6033):175-177
During the past few years, several methods have been developed for the detection of specific nucleic acid sequences by in situ hybridization using non-radioactive labels such as fluorochromes, cytochemically detectable enzymes and electron-dense markers. These methods are preferable to autoradiography in terms of speed of performance and topological resolution. Their limited sensitivity, however, has so far restricted their use to the detection of repeated sequences. Here we report single gene detection with a procedure using 2-acetylaminofluorene (AAF)-modified probes, immunoperoxidase cytochemistry and reflection-contrast microscopy. We confirmed the autoradiographic data on the localization of the human thyroglobulin (Tg) gene to the distal end of the long arm of chromosome 8. A mixture of cosmid cHT2-derived subclones of the 3' part of the Tg gene, 22.3 kilobase pairs (kbp) in total, was used as a hybridization probe. This procedure can be used to map other unique sequences, if genomic clones are available from which clones with an appropriate amount of inserts can be isolated. 相似文献
83.
84.
M. Harada S. Yenbutra K. Tsuchiya S. Takada 《Cellular and molecular life sciences : CMLS》1985,41(12):1610-1611
Summary Karyotypes ofMyotis siligorensis, Myotis mystacinus, Pipistrellus pulveratus, Tylonycteris robustula, Miniopterus schreibersi fuliginosus, Hipposideros fulvus andAselliscus stoliczkanus from Thailand are investigated.Acknowledgment. The authors are very grateful to Dr N. Ratanawarabhan, Mrs S. Sittilert, P. Noonpakdee and S. Kuanchalern of Thailand Institute of Scientific and Technological Research for their valuable advice and assistance during our field survey in Thailand. 相似文献
85.
一种改进的实信号IFFT算法 总被引:1,自引:0,他引:1
由于重量轻、体积小、功耗低、功能强的小卫星工作在无人干预的低温 ,高辐射的空间环境中 ,因此星上计算机系统必须满足集成度高、寿命长、可靠性高、抗辐射能力强的要求。针对立体测绘小卫星的特点 ,我们设计了满足上述要求的集集中控制与分布控制优点于一身的星上计算机系统 ,对其可靠性进行了分析 ,并对提高其可靠性应采取的关键技术进行了简要的论述。 相似文献
86.
Acquired immunity and epidemiology of Schistosoma haematobium 总被引:1,自引:0,他引:1
Human immune responses to schistosome infection have been characterized in detail. But there has been controversy over the relative importance of ecological factors (variation in exposure to infection) and immunological factors (acquired immunity) in determining the relationships between levels of infection and age typically found in areas where infection is endemic. Independent effects of exposure and age on the rates of reinfection with Schistosoma haematobium after chemotherapy have been demonstrated in the Gambia and Zimbabwe. This age effect could be the result of acquired immunity to infection. Indeed, allowing for variation in exposure and age, low rates of reinfection in the Gambia are correlated with high amounts of specific IgE antibodies--human IgE can kill S. mansoni schistosomulae in vitro. Further, animals can acquire immunologically mediated resistance to S. mansoni infection, although nonimmunological factors could also be involved. Acquisition of this immunity seems to be related to the cumulative effects of repeated infection and provides only partial protection. These characteristics are consistent with immuno-epidemiological data for both S. mansoni and S. haematobium infections of humans. We have now analysed age-prevalence data for human infection with S. haematobium, and find patterns of variation that are indeed consistent with the epidemiological effects of acquired immunity predicted by mathematical models. 相似文献
87.
钢琴副科课是我国音乐院校针对非钢琴专业的学生开设的一门钢琴课程.本文以如何上好钢琴副科课为探讨的主要目的,从基础和提高视谱能力这两个角度来展开讨论,力图使学生学习更富有热情和效率,对知识的掌握更为主动和全面. 相似文献
88.
J A Martignetti A A Aqeel W A Sewairi C E Boumah M Kambouris S A Mayouf K V Sheth W A Eid O Dowling J Harris M J Glucksman S Bahabri B F Meyer R J Desnick 《Nature genetics》2001,28(3):261-265
The inherited osteolyses or 'vanishing bone' syndromes are a group of rare disorders of unknown etiology characterized by destruction and resorption of affected bones. The multicentric osteolyses are notable for interphalangeal joint erosions that mimic severe juvenile rheumatoid arthritis (OMIMs 166300, 259600, 259610 and 277950). We recently described an autosomal recessive form of multicentric osteolysis with carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies in a number of consanguineous Saudi Arabian families. We localized the disease gene to 16q12-21 by using members of these families for a genome-wide search for homozygous-by-descent microsatellite markers. Haplotype analysis narrowed the critical region to a 1.2-cM region that spans the gene encoding MMP-2 (gelatinase A, collagenase type IV; (ref. 3). We detected no MMP2 enzymatic activity in the serum or fibroblasts of affected family members. We identified two family-specific homoallelic MMP2 mutations: R101H and Y244X. The nonsense mutation effects a deletion of the substrate-binding and catalytic sites and the fibronectin type II-like and hemopexin/TIMP2 binding domains. Based on molecular modeling, the missense mutation disrupts hydrogen bond formation within the highly conserved prodomain adjacent to the catalytic zinc ion. 相似文献
89.
研究以水泥处理固体有机物时,有机物对水泥水化的影响。以膜流模式说明水份在水泥浆体内传输的现象。水膜存在半干之水泥浆体,其厚度小于100nm,膜流由离分压所驱动,而离分压来自分子间作用力。膜拟结果发现,水泥浆体内局部位置可用以进行水化之水量为Q=-Aslv/6vπ。当有机物添加在水泥浆体内时,会影响Hamaker常数,Aslv因此可能使膜流不稳定或完全抑制膜流,因而使水泥浆体内局部缺水,影响水化进行,造成成品强度降低,影响品质。 相似文献
90.
林国顺 《哈尔滨商业大学学报(自然科学版)》1996,(3)
以计算机模拟为辅助工具 ,讨论了机器人路径规划的算法 ,提出一种简化算法二次规划 ,既可避免组合爆炸 ,又能得到一个较优的解 ,即满意解 相似文献