Integrin cytoplasmic tyrosine motif is required for outside-in alphaIIbbeta3 signalling and platelet function.

Integrins not only bind adhesive ligands, they also act as signalling receptors. Both functions allow the integrin alphaIIbbeta3 to mediate platelet aggregation. Platelet agonists activate alphaIIbbeta3 (inside-out signalling) to allow the binding of soluble fibrinogen. Subsequent platelet aggregation leads to outside-in alphaIIbbeta3 signalling, which results in calcium mobilization, tyrosine phosphorylation of numerous proteins including beta3 itself, increased cytoskeletal reorganisation and further activation of alphaIIbbeta3. Thus, outside-in signals enhance aggregation, although the mechanisms and functional consequences of specific signalling events remain unclear. Here we describe a mouse that expresses an alphaIIbbeta3 in which the tyrosines in the integrin cytoplasmic tyrosine motif have been mutated to phenylalanines. These mice are selectively impaired in outside-in alphaIIbbeta3 signalling, with defective aggregation and clot-retraction responses in vitro, and an in vivo bleeding defect which is characterized by a pronounced tendency to rebleed. These data provide evidence for an important role of outside-in signalling in platelet physiology. Furthermore, they identify the integrin cytoplasmic tyrosine motif as a key mediator of beta-integrin signals and a potential target for new therapeutic agents.     

钢板冷却过程分析与控制策略

钢板冷却工艺是影响钢板质量的重要因素。对钢板在某一风冷环境中的冷却过程进行研究,采用ANSYS软件建模,并对钢板的冷却过程进行模拟。分析钢板冷却过程的温度分布规律。远离钢板芯部温降较快,角部温降最剧烈,给出了采用活动挡板遮蔽形式的钢板沿宽度方向的均匀冷却控制策略。     

基于cox2-3 spacer序列的串珠藻属系统发育研究

对7种串珠藻属植物共13样本的cox2-3spacer序列进行了测定,并对其进行了系统发育分析.结果表明:cox2-3spacer序列片段长度为415bp,无插入或缺失,核苷酸变异位点238个,占序列长度的57%,其中178个简约信息位点,占序列长度的43%.A-T含量较多,进化上有碱基偏好性.胶串珠藻作为串珠藻属的模式种独立于其他串珠藻属植物,单独位于一个分支;长柄串珠藻、洪洞串珠藻和弧形串珠藻应该归入Sect.Helminthoidea;弯形串珠藻和绞扭串珠藻应归入新属Kumanoa.     

Ablation of cyclase-associated protein 2 (CAP2) leads to cardiomyopathy

Cyclase-associated proteins are highly conserved proteins that have a role in the regulation of actin dynamics. Higher eukaryotes have two isoforms, CAP1 and CAP2. To study the in vivo function of CAP2, we generated mice in which the CAP2 gene was inactivated by a gene-trap approach. Mutant mice showed a decrease in body weight and had a decreased survival rate. Further, they developed a severe cardiac defect marked by dilated cardiomyopathy (DCM) associated with drastic reduction in basal heart rate and prolongations in atrial and ventricular conduction times. Moreover, CAP2-deficient myofibrils exhibited reduced cooperativity of calcium-regulated force development. At the microscopic level, we observed disarrayed sarcomeres with development of fibrosis. We analyzed CAP2’s role in actin assembly and found that it sequesters G-actin and efficiently fragments filaments. This activity resides completely in its WASP homology domain. Thus CAP2 is an essential component of the myocardial sarcomere and is essential for physiological functioning of the cardiac system, and a deficiency leads to DCM and various cardiac defects.     

基于匹配分布和混合高斯模型的车辆检测算法

为解决高斯混合模型GMM（Gaussian Mixture Model）在车辆检测中存在的车辆断裂等问题, 提出了一种基于匹配度分布的混合高斯车辆检测算法。该算法采用c均值聚类法计算混合高斯模型初始值, 得到初步的背景模型； 匹配度分布的提出充分考虑了背景变化的时间性和空间性的特性； 根据前几帧检测结果得到每个点的匹配度分布, 对当前图片改变背景学习的规则, 去除了干扰, 适应了背景的变化。实验结果表明, 该算法较传统的混合高斯检测方法检测率平均提高16%以上, 使背景也更稳定和准确, 克服了车辆检测的断裂以及光照突变等问题, 提高了车辆区域检测的准确性。     

多层曲面仿生复眼成像系统的设计

针对单层曲面仿生复眼的子眼数目多, 难以加工以及所能达到的视场角较小的问题, 提出一种多层曲面仿生复眼结构。经过计算与分析得到, 当边缘子眼与中心子眼的光轴夹角为66.8°, 子眼的视场角为74.5°时, 利用7个子眼通道可实现仿生复眼系统180°范围内无盲区的成像, 减少了多个子眼复杂的加工过程。对单个子眼通道进行优化设计, 使其成像质量满足使用要求。实验表明, 该多层曲面仿生复眼结构能实现180°视场的成像, 且无成像盲区。