Crosstalk of protein kinase C ε with Smad2/3 promotes tumor cell proliferation in prostate cancer cells by enhancing aerobic glycolysis

Protein kinase C ε (PKCε) has emerged as an oncogenic protein kinase and plays important roles in cancer cell survival, proliferation, and invasion. It is, however, still unknown whether PKCε affects cell proliferation via glucose metabolism in cancer cells. Here we report a novel function of PKCε that provides growth advantages for cancer cells by enhancing tumor cells glycolysis. We found that either PKCε or Smad2/3 promoted aerobic glycolysis, expression of the glycolytic genes encoding HIF-1α, HKII, PFKP and MCT4, and tumor cell proliferation, while overexpression of PKCε or Smad3 enhanced aerobic glycolysis and cell proliferation in a protein kinase D- or TGF-β-independent manner in PC-3M and DU145 prostate cancer cells. The effects of PKCε silencing were reversed by ectopic expression of Smad3. PKCε or Smad3 ectopic expression-induced increase in cell growth was antagonized by inhibition of lactate transportation. Furthermore, interaction of endogenous PKCε with Smad2/3 was primarily responsible for phosphorylation of Ser213 in the Samd3 linker region, and resulted in Smad3 binding to the promoter of the glycolytic genes, thereby promoting cell proliferation. Forced expression of mutant Smad3 (S213A) attenuated PKCε-stimulated protein overexpression of the glycolytic genes. Thus, our results demonstrate a novel PKCε function that promotes cell growth in prostate cancer cells by increasing aerobic glycolysis through crosstalk between PKCε and Smad2/3.     

FD-3022四道γ能谱仪测量过程及常见问题浅谈

FD-3022能谱仪是铀资源勘查和核技术应用领域常见的一种γ能谱仪,广泛用于放射性矿床和其他金属和非金属矿床(如金矿、石油等)的野外普查找矿工作,也可做填图等其他地质研究、水文地质调查和环境监测等工作.仪器经标定后还可进行天然石材放射性测量.随着铀资源勘查任务的增加和核技术应用领域的拓展,仪器数量和使用人数不断增加,一些仪器故障、使用注意事项等困扰新手的问题也随之出现.错误的操作不仅影响了仪器的使用寿命,也耽误了项目进度.针对该仪器在计量检定和野外测量使用过程中常见的一些问题,进行了分析和归纳,并给出了参考的解决方法.     

Intraday volume percentages forecasting using a dynamic SVM-based approach

This paper proposes a dynamic model to forecast intraday volume percentages by decomposing the trade volume into two parts: The average part as the intraday volume pattern and the residual term as the abnormal changes. An empirical test on data spanning half-a-year gold futures and S&P 500 futures reveals that a rolling average of the previous days’ volume percentages shows great predictive ability for the average part. An SVM approach with the input pattern consisting of two categories is employed to forecast the residual term. One is the previous days’ volume percentages in the same time interval and the other is the most recent volume percentages. The study shows that this dynamic SVM-based forecasting approach outperforms the other commonly used statistical methods and enhances the tracking performance of a VWAP strategy greatly.     

An improvement to multiple criteria ABC inventory classification using Shannon entropy

This paper extends the Ng-model [Ng, 2007] for multiple criteria ABC inventory classification based upon Shannon entropy. The proposed approach determines the common weights associated with all criteria importance rankings, and provides a comprehensive scoring scheme by aggregating all rankings of the criteria importance. A numerical illustration is presented to compare the model with previous studies.     

A study on transport costs and China’s Exports: An extended gravity model

This paper suggests an extending conventional gravity model design to empirically analyze the effect of transport costs and port efficiency on China’s export flows. It shows that factor endowment and transport costs variables affect export trade value in directions that New Trade Theory (NTT) predicted. Also, the evidence indicates that, controlling for the effects of transport costs on trade, variables in traditional gravity model are consistent with previous empirical studies in both magnitudes and directions. Moreover, more than 22% of the variation in Chinese export trade can be explained by those three variables alone. The findings reported in this paper empirically explains how seriously transport costs and port efficiency affect China’s export growth by comparing effects of labour production factor costs on external trade. It suggests that the improvement of port efficiency and reduction of road transport costs play a vital role in China’s export competitiveness in the global market.     

cMonitor: VMI-based fine-grained monitoring mechanism in cloud

For the lack of detailed semantic in prior works, a transparent fine-grained monitoring technique （cMonitor） is pro- posed. Deployed outside the virtual machines, the cMonitor util- izes the elevated privileges of the virtual machine monitor to monitor the network connection, the processes and the relationship between them in protected systems by reconstructing fine-grained system semantics. These semantics contain process states and corresponding network connection. Experimental results show that cMonitor not only can be rapidly deployed in realistic cloud, but also can effectively and universally obtain these fine-grained semantics to assist detection of some advanced network attack. Meanwhile, the network performance overhead is about 3%, which is acceptable.     

基于遗传模糊系统的兵棋推演关键点推理方法

近年来,基于人工智能技术的自动化作战推演越发受到重视,但是由于有效地采集作战推演数据难度较大,许多依赖数据学习的人工智能技术效果不佳。在结合专家知识和作战推演数据的基础上研究作战推演中的人工智能技术,是一种可行的替代方案。为此,立足兵棋推演设计了关键点推理遗传模糊系统(genetic fuzzy system, GFS)框架,有效整合了对兵棋专家知识的建模和对兵棋复盘数据的学习,从而提高了关键点的推理质量。进一步以安全点为例,构建了安全点推理GFS,在初始化模糊系统规则库的基础上,通过合理设计遗传算法中的参数编码、适应度函数、遗传算子等,实现了安全点推理模糊系统的遗传调优算法。最后,通过实验仿真展示了所提方法的可行性和实用性。     

一类非线性扰动波方程的渐近理论及应用

对二阶半线性波动方程的初值问题，本文将给出一个渐近方法。初值问题的适定性及形式近似解的合理性都在时间变量无穷大时成立。作为渐近理论的应用。本文对带初值问题的Ｒａｙｌｅｉｇｈ波方程进行了详细的讨论。     

金边吊兰叶细胞超微结构的观察

用透射电镜对金边吊兰地细胞进行了观察，发现同一叶片绿色部分细胞的叶绿体结构正常，黄色部分的细胞没有正常的叶绿体，只有停止发育和退化解体的质体，并对此现象进行了分析。     

一步拷贝法二维真彩色全息图

介绍一种只用单波长激光作一步拷贝记录来拍摄二维真彩色全息图的方法．本方法比起其它方法具有记录过程简单及成本低的优点，而且重现的全息图像比用其它方法制作的全息图像清晰明亮得多．