Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis

To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.     

中医妇科课程案例教学法的应用

研究如何改进教学方法,是每一个任课教师的责任,本文阐述了案例教学在中医妇科学教学中的作用及如何将案例教学融入特殊教育等问题。     

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研究双馈电机运行速度对双馈电机稳态性能的影响．首先对比了双馈电机运行于超同步转速及次同步转速时的稳态波形，然后对谐波及稳态参数的变化进行了分析．研究表明双馈电机稳态运行速度对其稳态运行性能有较大影响．     

电子碰撞Ag的L壳层电离截面的测量

作者用Si(Li)探测器测量了能量为5～2keV的电子碰撞Ag的L壳总平均电离截面；用Monte Carlo方法计算的电子反射能谱，修正了来自衬底的反射电子对测量结果的影响．作者对实验结果与Cryzinski的理论计算结果进了比较．     

岩爆的力学模型及物理数值模拟述评

结合现有的岩爆研究情况，对岩爆发生机制的力学模型以及在室内实验和数值模拟方面所做的工作进行总结，介绍可资岩爆借鉴的研究领域，提出岩爆的研究方向。     

Metamorphic Algorithm of Self-reconfigurable Modular Robotic System

Introduction Aself reconfigurableroboticsystemconsistsofman simpleidenticalmechatronicmodules.Thesemodulesca connectanddisconnectfromonetoanothertochangethe overalltopology.Thesystemcanautonomouslyandynamicallyadaptitsshapetosuittheneedsofthetask hand,e.g.manipulation,locomotion,andcreationo staticstructures.Thegeneralphilosophyofsel reconfigurableroboticsystemistosimplifythedesignanreducemanufacturingcostandenhancefunctionalityan versatilitythroughalargernumberofidenticalmodules.I addition…     

基层图书馆工作现状及对策

针对新时期基层图书馆所面临的经费不足、体制僵化、管理观念陈旧等一系列问题，提出了使基层图书馆走出国境、适应新时代发展需要的若干对策。     

一种高级计算机语言──SAS语言初探

扼要介绍了ＳＡＳ语言及其关键步（ＤＡＴＡ步和ＰＲＯＣ步）．给出了几个例子，以说明如何使用ＳＡＳ语言。     

整机老炼条件的优化设计

本文通过工程实例，阐述如何利用现有信息来设计产品的老炼条件，以使它在保证产品达到规定可靠性的前提下尽量节省成本     

Pb-Sn-Bi三元系α相固溶体的点阵参数

应用X射线衍射法测定了Pb-Sn-Bi三元系Pb基α相固溶体的点阵参数,发现点阵间距与成分呈线性变化,对两批实验数据分别进行回归分析,得到点阵参数-浓度关系的统一解析式: α=0.49495X_(Pb)+0.47813X_(Sn)+0.50629X_(Bi)。其F检验置信度大于99.9％,残余标准差σ=6.4×10~(-5) nm,分析点阵畸变的影响因素表明尺寸效应是控制固溶体点阵行为的主要因素。