Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

"溶液吸附法测定活性炭比表面积"实验的改进

研究了吸附荆的颗粒度、吸附时间、吸附温度、原始溶液浓度对吸附平衡的影响，确定了新的实验条件：粒状活性炭破碎至60．100目；70℃恒温振荡0．5h后，再于室温振荡吸附1．5h；原始溶液的溶质质量分数为2％左右．改进后，提高了实验精密度，缩短了实验时间．     

Microstructure transition of Al-Cu eutectic alloy during containerless rapid solidification

Droplets of Al-32.7%Cu eutectic alloy were rapidly solidified during containerless processing in a 3 m drop tube. The microstructural evolution of Al-Cu eutectic alloy depends strongly on droplet size. The eutectic growth morphology changes from regular lamellar eutectic to a kind of anomalous eutectic with the decrease in droplet size. This microstructural transition was analyzed within the current theories of eutectic growth. The results indicate that there exists a critical velocity for eutectic growth beyond which anomalous eutectic appears. The coupled zone of the Al-Cu eutectic alloy system has been calculated on the basis of the Trivedi-Magnin-Kurz(TMK) eutectic growth and the Lipton-Kurz-Trivedi(LKT)/Boettinger-Coriell-Trivedi(BCT) dendrite growth models, which provides a further interpretation for the morphological transition.     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.     

峰谷电价体制下东北输油管网日输油优化研究

东北原油长输管网是中国规模最大的地区性原油运输系统，就该管网基于峰谷电价体制下的运行现状，建立了线性规划数学模型，提出了日输油计划优化问题，并以铁秦线为例进行分析；初步揭示了峰谷电价体制下日输油计划方式的一些基本规律， 表明在该体制下东北管网具有一定的节能降耗潜力。但该方式在一定程度上受到管道本身运行特性的约束，与热油管道相比它更适合于等温管道。     

Spectroscopic study on the photophysical properties of chlorine substituted tetraphenylporphyrin-histidine and its zinc(Ⅱ)complexes

As the structural analogues of (bacterio)chlorophylls in photosynthetic organisms, porphyrins play an important role in building up artificial photosynthetic systems[13]. In natural photosynthetic pigment-protein complexes,certain amino acid residuals modulate the reactions ofcharge transfer and energy transfer via coordination andother types of interactions with chlorophyll molecules.For instance, in higher-plant photosynthetic reaction cen-ter PSⅡ, the oxidization-reduction system consistin…     

思想政治教育教学应注意的几个问题

文章旨在从教育教学的角度，从六个方面探讨新形势下如何加强和改进思想政治教育教学的方法，以提高思想政治教育教学工作的效率，实现思想政治教育的功能。     

一个广义Rough集模型及其性质

在传统Rough集理论基础上建立了一个广义Rough集模型，并研究了它的有关性质。