Molecular cloning and expression of interleukin Ibeta (IL-1β) from red seabream (Pagrus major)

The interleukin 1β (IL-1β) cDNA was cloned from the red seabream (Pagrus major) by homology cloning strategy. A cDNA fragment was amplified by PCR using two degenerated primers, which were designed according to the conserved regions of other known IL-1β sequences, and elongated by 3' ends and 5' ends RACE PCR to get the full length coding sequence of red seabream IL-1β (RS IL-1β). The sequence contained 1252 nucleotides that included a 5' untranslated region (UTR) of 84bp, a 3' UTR of 410 bp and an open reading frame (ORF) of 759 nucleotides which could be translated into a putative peptide of 253 amino acids with molecular weight of 28.6 kD and putative isoelectric point pI of 5.29. The deduced peptide contained two potential N-glycosylation sites and an identifiable IL1 family signature, but lacked the signal peptide and the clear ICE cut site, which were common in other nonmammalian IL-1β genes. The RS IL-1β had the highest homology with piscine IL-1β according to phylogenetic tree analysis.     

中日两国在双边关系中的主动与被动

新中国成立以来，在中日关系发展的不同阶段，中国一直处于主动地位，积极推动双边关系的发展。而日本则时而主动，时而被动，时而同时具有主动与被动的两面性。要使中日关系朝着友好合作伙伴的方向健康发展，同需两国的积极主动。     

LabVIEW在曲线拟合中的应用研究

介绍了LabVIEW中用于曲线拟合的子模块，对各子模块的曲线拟合理论作了介绍，重点介绍了非线性Lev-Mar拟合理论，并以例子辅助说明．     

Development of Full Automatic External Welding Machine for Annular Welds of Pipeline

To meet the demands of rapid development of pipeline constructions in China, a complete set of full automatic external welding machine for annular welds of pipeline and welding procedure have been developed thereof. The system has been applied in the “West-East Pipeline Project” and its performance is proven. With this welding machine the welding parameters can be either preset before or adjusted in real time during the welding process, which avoids the blemishes of same type domestic and international products. Thus it is best propitious to in-situ construction conditions and workers‘ technical level in China. In this article the development of mechanical and control systems of this product is introduced and typical welding procedure parameters are provided.     

关于艺术设计中基础课程设置的思考

目前,我国的设计教育基础课程的设置仍存在很大的问题.基础课程的设置,应该成为艺术设计学科建设的焦点和重点.艺术设计基础课程,应把重点放在意识的培养与技术的训练上.根据我国现有设计专业的特点,应该设置理论基础课和专业基础课两类艺术设计基础课程.     

制备介孔复合环境材料的酸洗刻蚀工艺

探索了一种制备介孔复合环境材料的方法--破坏式造孔与有机复合相结合,使最终产品可大量吸附污染水体中的各种污染物并具有一定的杀菌功能.该方法通过酸洗刻蚀,达到了去除原材料中的杂质和刻蚀孔径的目的,所制备的中间产品24h吸湿率为原材料的2倍,染料吸附量为原材料的1.6倍.     

木材在机械加工中崩茬的控制

在木材的机械加工过程中，崩茬是比较容易出现的制造缺陷，如何有效的控制崩茬问题的出现是非常重要的。本文主要分析崩茬的原因以及平行四边形法则在控制崩茬问题上的应用。     

英汉词语的语义对应与翻译

英汉词汇的语义对应是双语翻译的前提,是两种语言相互转换的桥梁.但是由于受词汇用法、语言环境和文化背景差异等因素的制约,这种对应往往被简单地曲解成了对等.要实现语言的正确理解和翻译,应注意在英译汉的过程中摆脱语义对等的困扰,使译文在词汇意义这一层面上更贴近原文,以最大限度地实现科学的转换.     

基于主客体作用的荧光传感器研究进展

基于主客体作用的荧光传感器研究备受人们关注，近年来得到了迅速发展。本介绍了以冠醚、环糊精、杯芳烃等大环化合物作为接受体的荧光传感器的制备、分子离子识别机制和应用研究现状，展望了未来的发展趋势。     

极低硫钢的精炼脱硫动力学模型

通过对影响脱硫反应限制性环节的分析,将持续接触和瞬时接触两种反应模式综合考虑,建立了精炼脱硫动力学模型,并通过实验室"极低硫钢精炼脱硫"实验数据对模型进行验证.结果表明,本模型的预测结果与实验结果比较吻合.