全文获取类型
收费全文 | 676篇 |
免费 | 17篇 |
国内免费 | 10篇 |
专业分类
系统科学 | 28篇 |
丛书文集 | 48篇 |
教育与普及 | 2篇 |
理论与方法论 | 4篇 |
现状及发展 | 88篇 |
研究方法 | 12篇 |
综合类 | 495篇 |
自然研究 | 26篇 |
出版年
2022年 | 5篇 |
2021年 | 9篇 |
2020年 | 8篇 |
2019年 | 8篇 |
2018年 | 17篇 |
2017年 | 20篇 |
2016年 | 13篇 |
2015年 | 19篇 |
2014年 | 23篇 |
2013年 | 23篇 |
2012年 | 31篇 |
2011年 | 40篇 |
2010年 | 26篇 |
2009年 | 31篇 |
2008年 | 36篇 |
2007年 | 47篇 |
2006年 | 59篇 |
2005年 | 51篇 |
2004年 | 53篇 |
2003年 | 21篇 |
2002年 | 27篇 |
2001年 | 20篇 |
2000年 | 11篇 |
1999年 | 5篇 |
1997年 | 3篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1984年 | 2篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1974年 | 5篇 |
1973年 | 3篇 |
1972年 | 3篇 |
1971年 | 5篇 |
1970年 | 2篇 |
1968年 | 4篇 |
1967年 | 2篇 |
1965年 | 3篇 |
1963年 | 2篇 |
1962年 | 1篇 |
1961年 | 1篇 |
1959年 | 2篇 |
1958年 | 8篇 |
1957年 | 4篇 |
1956年 | 6篇 |
1955年 | 6篇 |
1954年 | 10篇 |
1948年 | 2篇 |
排序方式: 共有703条查询结果,搜索用时 446 毫秒
11.
单片机程序的限时服务策略及设计 总被引:9,自引:0,他引:9
针对单片机软件中由于任务超时导致的运行紊乱情况,提出了一种借助硬件的定时中断机制,只需只量资源就可实现的限时服务处理方法,并针对顺序操作和任务操作两种情况进行详细说明。能够防止和解决程序中出现因条件不满足而无法退出功能模块,出现独占MCU时间,以及多任务机制下的任务重叠,使资源间相互干扰而出现死循环状态或运行状态错误等问题,从而提高系统的冗余度和可靠性。 相似文献
12.
大力推进信息化,以信息化带动工业化,是湖南完成工业化任务,发挥后发优势,实现生产力跨越式发展的新机遇.因此,深入研究和积极探索以信息化带动工业化的有效途径和方式,是摆在我们面前的一项重要任务. 相似文献
13.
The interactions between plants and their animal pollinators and seed dispersers have moulded much of Earth's biodiversity. Recently, it has been shown that these mutually beneficial interactions form complex networks with a well-defined architecture that may contribute to biodiversity persistence. Little is known, however, about which ecological and evolutionary processes generate these network patterns. Here we use phylogenetic methods to show that the phylogenetic relationships of species predict the number of interactions they exhibit in more than one-third of the networks, and the identity of the species with which they interact in about half of the networks. As a consequence of the phylogenetic effects on interaction patterns, simulated extinction events tend to trigger coextinction cascades of related species. This results in a non-random pruning of the evolutionary tree and a more pronounced loss of taxonomic diversity than expected in the absence of a phylogenetic signal. Our results emphasize how the simultaneous consideration of phylogenetic information and network architecture can contribute to our understanding of the structure and fate of species-rich communities. 相似文献
14.
Oxysterols direct immune cell migration via EBI2 总被引:1,自引:0,他引:1
Hannedouche S Zhang J Yi T Shen W Nguyen D Pereira JP Guerini D Baumgarten BU Roggo S Wen B Knochenmuss R Noël S Gessier F Kelly LM Vanek M Laurent S Preuss I Miault C Christen I Karuna R Li W Koo DI Suply T Schmedt C Peters EC Falchetto R Katopodis A Spanka C Roy MO Detheux M Chen YA Schultz PG Cho CY Seuwen K Cyster JG Sailer AW 《Nature》2011,475(7357):524-527
Epstein-Barr virus-induced gene 2 (EBI2, also known as GPR183) is a G-protein-coupled receptor that is required for humoral immune responses; polymorphisms in the receptor have been associated with inflammatory autoimmune diseases. The natural ligand for EBI2 has been unknown. Here we describe the identification of 7α,25-dihydroxycholesterol (also called 7α,25-OHC or 5-cholesten-3β,7α,25-triol) as a potent and selective agonist of EBI2. Functional activation of human EBI2 by 7α,25-OHC and closely related oxysterols was verified by monitoring second messenger readouts and saturable, high-affinity radioligand binding. Furthermore, we find that 7α,25-OHC and closely related oxysterols act as chemoattractants for immune cells expressing EBI2 by directing cell migration in vitro and in vivo. A critical enzyme required for the generation of 7α,25-OHC is cholesterol 25-hydroxylase (CH25H). Similar to EBI2 receptor knockout mice, mice deficient in CH25H fail to position activated B cells within the spleen to the outer follicle and mount a reduced plasma cell response after an immune challenge. This demonstrates that CH25H generates EBI2 biological activity in vivo and indicates that the EBI2-oxysterol signalling pathway has an important role in the adaptive immune response. 相似文献
15.
Peça J Feliciano C Ting JT Wang W Wells MF Venkatraman TN Lascola CD Fu Z Feng G 《Nature》2011,472(7344):437-442
Autism spectrum disorders (ASDs) comprise a range of disorders that share a core of neurobehavioural deficits characterized by widespread abnormalities in social interactions, deficits in communication as well as restricted interests and repetitive behaviours. The neurological basis and circuitry mechanisms underlying these abnormal behaviours are poorly understood. SHANK3 is a postsynaptic protein, whose disruption at the genetic level is thought to be responsible for the development of 22q13 deletion syndrome (Phelan-McDermid syndrome) and other non-syndromic ASDs. Here we show that mice with Shank3 gene deletions exhibit self-injurious repetitive grooming and deficits in social interaction. Cellular, electrophysiological and biochemical analyses uncovered defects at striatal synapses and cortico-striatal circuits in Shank3 mutant mice. Our findings demonstrate a critical role for SHANK3 in the normal development of neuronal connectivity and establish causality between a disruption in the Shank3 gene and the genesis of autistic-like behaviours in mice. 相似文献
16.
Role of sulphuric acid, ammonia and galactic cosmic rays in atmospheric aerosol nucleation 总被引:1,自引:0,他引:1
Kirkby J Curtius J Almeida J Dunne E Duplissy J Ehrhart S Franchin A Gagné S Ickes L Kürten A Kupc A Metzger A Riccobono F Rondo L Schobesberger S Tsagkogeorgas G Wimmer D Amorim A Bianchi F Breitenlechner M David A Dommen J Downard A Ehn M Flagan RC Haider S Hansel A Hauser D Jud W Junninen H Kreissl F Kvashin A Laaksonen A Lehtipalo K Lima J Lovejoy ER Makhmutov V Mathot S Mikkilä J Minginette P Mogo S Nieminen T Onnela A Pereira P Petäjä T Schnitzhofer R Seinfeld JH Sipilä M Stozhkov Y 《Nature》2011,476(7361):429-433
Atmospheric aerosols exert an important influence on climate through their effects on stratiform cloud albedo and lifetime and the invigoration of convective storms. Model calculations suggest that almost half of the global cloud condensation nuclei in the atmospheric boundary layer may originate from the nucleation of aerosols from trace condensable vapours, although the sensitivity of the number of cloud condensation nuclei to changes of nucleation rate may be small. Despite extensive research, fundamental questions remain about the nucleation rate of sulphuric acid particles and the mechanisms responsible, including the roles of galactic cosmic rays and other chemical species such as ammonia. Here we present the first results from the CLOUD experiment at CERN. We find that atmospherically relevant ammonia mixing ratios of 100 parts per trillion by volume, or less, increase the nucleation rate of sulphuric acid particles more than 100-1,000-fold. Time-resolved molecular measurements reveal that nucleation proceeds by a base-stabilization mechanism involving the stepwise accretion of ammonia molecules. Ions increase the nucleation rate by an additional factor of between two and more than ten at ground-level galactic-cosmic-ray intensities, provided that the nucleation rate lies below the limiting ion-pair production rate. We find that ion-induced binary nucleation of H(2)SO(4)-H(2)O can occur in the mid-troposphere but is negligible in the boundary layer. However, even with the large enhancements in rate due to ammonia and ions, atmospheric concentrations of ammonia and sulphuric acid are insufficient to account for observed boundary-layer nucleation. 相似文献
17.
Ricardo F. Antunes Cláudia Brandão Gonçalo Carvalho Cristina Girão Fernando A. Arosa 《Cellular and molecular life sciences : CMLS》2009,66(20):3387-3398
Red blood cells (RBC) have emerged as a novel regulatory cell type endowed with bioactivities toward activated human T cells.
Herein we show that the RBC bioactivities act on intracellular pathways initiated by T cell receptor (TCR)-dependent and -independent
stimuli, including IL-2, IL-15, and the mixture of phorbol dibutyrate and ionomycin. The RBC bioactivities preserve the antioxidant
status and are capable of rescuing activated T cells from cell death induced by serum deprivation. They are not mediated by
glycosylphosphatidylinositol-linked receptors or sialic acids, and kinetic studies revealed that they hasten the entrance
into the cell cycle. By using cyclosporine A (CsA) and rapamycin (Rapa) we show that the RBC bioactivities are calcineurin-dependent.
Thus, treatment of T cells with CsA, but not Rapa, impaired RBC bioactivities, and preincubation of RBC with CsA completely
abolished their bioactivities. We have demonstrated that RBC carry out bioactivities that are sensitive to CsA. 相似文献
18.
The West Palearctic species of Rhoptromeris are revised. A total of 11 species are recognised as valid in this region, including four newly described species: Rhoptromeris dichromata sp. nov., Rhoptromeris koponeni sp. nov., Rhoptromeris leptocornis sp. nov. and Rhoptromeris macaronesiensis sp. nov. Eucoila luteicornis Ionescu, 1959 is synonymised with Rhoptromeris heptoma (Hartig, 1840) syn. nov. A checklist of the Holarctic Rhoptromeris is presented and an identification key to the West Palearctic species is provided. www.zoobank.org/urn:lsid:zoobank.org:pub:8164332C-93E2-4E3F-A408-F5FF5DFB366E 相似文献
19.
Irina Kerkis Alvaro Rossan de Brandão Prieto da Silva Celine Pompeia Jan Tytgat Paulo L. de Sá Junior 《Cellular and molecular life sciences : CMLS》2017,74(4):647-661
Toxins have been shown to have many biological functions and to constitute a rich source of drugs and biotechnological tools. We focus on toxins that not only have a specific activity, but also contain residues responsible for transmembrane penetration, which can be considered bioportides—a class of cell-penetrating peptides that are also intrinsically bioactive. Bioportides are potential tools in pharmacology and biotechnology as they help deliver substances and nanoparticles to intracellular targets. Bioportides characterized so far are peptides derived from human proteins, such as cytochrome c (CYCS), calcitonin receptor (camptide), and endothelial nitric oxide synthase (nosangiotide). However, toxins are usually disregarded as potential bioportides. In this review, we discuss the inclusion of some toxins and molecules derived thereof as a new class of bioportides based on structure activity relationship, minimization, and biological activity studies. The comparative analysis of the amino acid residue composition of toxin-derived bioportides and their short molecular variants is an innovative analytical strategy which allows us to understand natural toxin multifunctionality in vivo and plan novel pharmacological and biotechnological products. Furthermore, we discuss how many bioportide toxins have a rigid structure with amphiphilic properties important for both cell penetration and bioactivity. 相似文献
20.
Marcelo S. Perlin João F. Caldeira André A. P. Santos Martin Pontuschka 《Journal of forecasting》2017,36(4):454-467
We look into the interaction of Google's search queries and several aspects of international equity markets. Using a novel methodology for selecting words and a vector autoregressive modeling approach, we study whether the search queries of finance‐related words can have an impact on returns, volatility of returns and traded volume in four different English‐speaking countries. We identify several words whose search frequency is associated with changes in the dependent variables. In particular, we find that increases in search queries including the word stock predict increased volatility and decreased index returns over the next week. On top of that, we investigate the performance of a market‐timing strategy based on the search frequency of this word and benchmark it against random words from the Word‐Net database and a naive buy‐and‐hold strategy. The results of this empirical application are positive and particularly stronger during the global crisis of 2009. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献