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31.
During the evolution of proteins the pressure to optimize biological activity is moderated by a need for efficient folding. For most proteins, this is accomplished through spontaneous folding to a thermodynamically stable and active native state. However, in the extracellular bacterial alpha-lytic protease (alphaLP) these two processes have become decoupled. The native state of alphaLP is thermodynamically unstable, and when denatured, requires millennia (t1/2 approximately 1,800 years) to refold. Folding is made possible by an attached folding catalyst, the pro-region, which is degraded on completion of folding, leaving alphaLP trapped in its native state by a large kinetic unfolding barrier (t1/2 approximately 1.2 years). alphaLP faces two very different folding landscapes: one in the presence of the pro-region controlling folding, and one in its absence restricting unfolding. Here we demonstrate that this separation of folding and unfolding pathways has removed constraints placed on the folding of thermodynamically stable proteins, and allowed the evolution of a native state having markedly reduced dynamic fluctuations. This, in turn, has led to a significant extension of the functional lifetime of alphaLP by the optimal suppression of proteolytic sensitivity. 相似文献
32.
A. E. Yassen H. A. Hassan L. S. Kawashti 《Cellular and molecular life sciences : CMLS》1994,50(11-12):1111-1114
A detailed karyotypic analysis of two Egyptian species of bats,Taphozous perforatus andTaphozous nudiventris, was made on the basis of conventional data and G-band patterns. No detectable karyotypic difference was found between the two species (2n=42, F.N. 64). By comparison of G-band patterns, some differences between the two species were seen in the corresponding autosomal pairs. These results are reported for the first time in Egypt. 相似文献
33.
The gas-liquid-liquid reactive extraction system for preparing hydrogen peroxide via anthraquinone was investigated. The oxidation reaction of hydrogenated working solution was combined with the extraction of hydrogen peroxide from working solution in a sieve plate column. The reaction of 2-ethylanthrahydroquionone with oxygen and the liquid-liquid extraction of hydrogen peroxide take place simultaneously. The oxygen was introduced with hydrogenated working solution through a nozzle in the bottom of the column, which worked as agitated air as well as oxidation reagent. The results showed the oxidation and extraction do not hamper each other, on the contrary, the presence of oxidation gas in the column can promote the transfer of hydrogen peroxide from organic phase to aqueous phase, thus the reaction efficiency and extraction efficiency increased with increasing gas superficial velocity. Furthermore, the oxidation efficiency is almost 100% and the extraction efficiency is higher than 90% in this process. 相似文献
34.
35.
提出了一个关于氧化物半导瓷晶界势垒起源的新观点,认为晶界势垒起源于烧结过程中外界氧在晶界中的扩散,与材料的结构、化学缺陷、掺杂、外界气氛、烧结工艺、组成状态等有密切关系,并用此理论解释了许多实验现象。 相似文献
36.
论述了交织编码的工作原理,给出了一种交织扩展Golay码的改进型软件设计方案,用TMS320C25实现了其编译码器并给出了模拟实验结果。实验结果表明,它能准确地纠正不大于72比特位的差错;处理128×128×8比特位的一幅图像数据的运行时间为0.412s. 相似文献
37.
38.
史济群 《华中科技大学学报(自然科学版)》1994,(3)
报道一种新颖的激光开槽、埋槽电极硅太阳电池的结构、工艺流程及其研制结果。在AM1.5,25℃,100mW/cm2的条件下,以面积为45cm3的36片硅片研制的硅太阳电池的输出参数的平均值为JSC=36.1mA/cm2,V∞=633mV,F.F.=0.798,η=18.23%,最后分析了种高性能硅太阳电池的设计特点。 相似文献
39.
对目前的UBBE模型进行了适当的推广,使在解决估计问题时可以考虑那些可能是误差上界的数值,并在此基础上提出了一种方法,能够通过对估计精度和可靠性进行合理的权衡确定所需估计值,实际案例研究结果表明所提方法能够较好地解决估计精度和可靠性之间的矛盾。 相似文献
40.
吕聆春 《佛山科学技术学院学报(自然科学版)》2006,24(2):60-63
提出了一种基于IP多播技术的解决方案并加以实现,满足了对上机学生按系或班级分组进行管理与各类统计的需要,实现了传统C/S结构所能实现的全部功能,解决了传统机房信息管理系统使用带来的问题。 相似文献