Structural Changes of Water Molecules Upon the Reduction of Quinones in The Reaction Center from Rhodobactery Sphaeroides

1 Results The photosynthetic bacterial reaction center (RC) is a membrane protein complex.The RC is composed of three protein subunits and redox components such as bacteriochlorophylls, bacteriopheophytins,and quinones.The RC performs the photochemical electron transfer from the bacteriochlorophyll dimer through a series of electron donor and acceptor molecules to a secondary quinone,QB.QB accepts electrons from a primary quinone,QA,in two sequential electron transfer reactions.The second electron trans...     

A non-commutative time-frequency tomography

The characterization of non-stationary signal requires joint time and frequency information. However, time and frequency are a pair of non-commuting variables that cannot constitute a joint probability density in the time-frequency plane. The time-frequency distributions have difficult interpretation problems arising from negative and complex values or spurious components. In this paper, we get time-frequency information from the marginal distributions in rotated directions in the time-frequency plane. The rigorous probability interpretation of the marginal distributions is without any ambiguities. This time-frequency transformation is similar to the computerized axial tomography (CT or CAT) and is applied to signal analysis and signal detection and reveals a lot of advantages especially in the signal detection of the low signal/noise (S/N).     

浅谈随刊附盘的著录与管理

介绍了随刊附盘的种类及其验收与著录的方法,提出了随刊附盘管理和利用的措施。     

不同药液浸泡树棒培养蜜环菌材试验

本文对六种不同药液浸泡树棒培养密环菌菌材进行了研究，结果表明：0．5ppm三十烷醇加300ppm单宁酸的混合液浸泡树棒，培养蜜环菌，其接菌率高、菌生长势好，明显优于沿用已久的0．25％硝酸接溶液浸泡树棒培养密菌菌材的方法。     

Transgenesis in fish

Gene transfer into fish embryo is being performed in several species (trout, salmon, carps, tilapia, medaka, goldfish, zebrafish, loach, catfish, etc.). In most cases, pronuclei are not visible and microinjection must be done into the cytoplasm of early embryos. Several million copies of the gene are generally injected. In medaka, transgenesis was attempted by injection of the foreign gene into the nucleus of oocyte. Several reports indicate that the injected DNA was rapidly replicated in the early phase of embryo development, regardless of the origin and the sequence of the foreign DNA. The survival of the injected embryos was reasonably good and a large number reached maturity. The proportion of transgenic animals ranged from 1 to 50% or more, according to species and to experimentators. The reasons for this discrepancy have not been elucidated. In all species, the transgenic animals were mosaic. The copy number of the foreign DNA was different in the various tissues of an animal and a proportion lower than 50% of F1 offsprings received the gene from their parents. This suggests that the foreign DNA was integrated into the fish genome at the two cells stage or later. An examination of the integrated DNA in different cell types of an animal revealed that integration occurred mainly during early development. The transgene was found essentially unrearranged in the fish genome of the founders and offsprings. The transgenes were therefore stably transmitted to progeny in a Mendelian fashion. Southern blot analysis revealed the presence of possible junction fragments and also of minor bands which may result from a rearrangement of the injected DNA. In all species, the integrated DNA appeared mainly as random end-to-end concatemers. In adult trout blood cells, a small proportion of the foreign DNA was maintained in the form of non-integrated concatemers, as judged by the existence of end fragments. The transgenes were generally only poorly expressed. The majority of the injected gene constructs contained essentially mammalian or higher vertebrates sequences. The comparison of the expression efficiency of these constructs in transfected fish and mammalian cells indicates that some of the mammalian DNA sequences are most efficiently understood by the fish cell machinery. Chloramphenicol acetyl transferase gene under the control of promoters from Rous sarcoma virus, and human cytomegalovirus, was expressed in several tissues of transgenic fish. Chicken delta-crystallin gene was expressed in several tissues of transgenic fish.(ABSTRACT TRUNCATED AT 400 WORDS)     

加强党的执政能力建设 实施“有限政府”治国

阐述了在国内外形势发生急剧变化和我国全面建设小康社会进入紧要关头的犬背景下。加强我党执政能力建设的重要性和必要性，分析了传统“全能政府”的弊端，指出“有限政府”治国是使国家和政府有所作为的最好办法，建立“有限政府”应是我国政治体制改革的一个重要目标。     

Duane模型、分组数据的AMSAA模型和具丢失数据的AMSAA模型——Bayes分析方法

本文将Bayes分析方法应用于Duane可靠性增长模型、分组数据的AMSAA模型和具丢失数据的AMSAA模型,得到了参数以及系统能达到的MTBF的Bayes点估计和Bayes区间估计。     

多孔硅的形成及可见光发射

多孔硅是晶体硅片在氢氟酸溶液中进行阳极氧化，在硅衬底上形成多孔态的硅材料。本文介绍了多孔硅的形成和结构形貌，对其光学性质和发光机制进行了扼要讨论，并介绍了当前多孔硅研究中的一些热点问题。     

分批加权优化的排课模型

本文介绍一种分批加权优化的高校排课模式．这一模式无需要使用探试方法，因此它优于一般采用的人工智能方法．