首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   18504篇
  免费   103篇
  国内免费   273篇
系统科学   397篇
丛书文集   398篇
教育与普及   72篇
理论与方法论   62篇
现状及发展   6248篇
研究方法   776篇
综合类   10406篇
自然研究   521篇
  2014年   157篇
  2013年   293篇
  2012年   511篇
  2011年   1060篇
  2010年   404篇
  2009年   385篇
  2008年   576篇
  2007年   671篇
  2006年   678篇
  2005年   591篇
  2004年   432篇
  2003年   327篇
  2002年   334篇
  2001年   527篇
  2000年   577篇
  1999年   423篇
  1992年   288篇
  1991年   252篇
  1990年   250篇
  1989年   229篇
  1988年   209篇
  1987年   226篇
  1986年   203篇
  1985年   245篇
  1984年   235篇
  1983年   159篇
  1982年   172篇
  1981年   157篇
  1980年   199篇
  1979年   445篇
  1978年   340篇
  1977年   367篇
  1976年   264篇
  1975年   322篇
  1974年   446篇
  1973年   379篇
  1972年   360篇
  1971年   421篇
  1970年   484篇
  1969年   425篇
  1968年   407篇
  1967年   442篇
  1966年   384篇
  1965年   301篇
  1959年   155篇
  1958年   231篇
  1957年   179篇
  1956年   138篇
  1955年   154篇
  1954年   132篇
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
971.
972.
973.
The potential use of smallpox as a biological weapon has led to the production and stockpiling of smallpox vaccine and the immunization of some healthcare workers. Another public health goal is the licensing of a safer vaccine that could benefit the millions of people advised not to take the current one because they or their contacts have increased susceptibility to severe vaccine side effects. As vaccines can no longer be tested for their ability to prevent smallpox, licensing will necessarily include comparative immunogenicity and protection studies in non-human primates. Here we compare the highly attenuated modified vaccinia virus Ankara (MVA) with the licensed Dryvax vaccine in a monkey model. After two doses of MVA or one dose of MVA followed by Dryvax, antibody binding and neutralizing titres and T-cell responses were equivalent or higher than those induced by Dryvax alone. After challenge with monkeypox virus, unimmunized animals developed more than 500 pustular skin lesions and became gravely ill or died, whereas vaccinated animals were healthy and asymptomatic, except for a small number of transient skin lesions in animals immunized only with MVA.  相似文献   
974.
Each cone photoreceptor in the retina responds to light in a limited range of wavelengths, giving it a spectral phenotype. This phenotype is determined by the most prevalent of the photoreceptor's visual-pigment proteins (opsins) and is assumed to remain unchanged during an animal's lifetime. Here we show that in the Pacific pink salmon, Oncorhynchus gorbuscha, single cones can switch their spectral phenotype from ultraviolet to blue by regulating the production of the appropriate opsins as the fish grow older. This photoreceptor plasticity may operate to modulate colour vision as the salmon's lifestyle changes.  相似文献   
975.
976.
Pierce JL  Meyer GA  Jull AJ 《Nature》2004,432(7013):87-90
Western US ponderosa pine forests have recently suffered extensive stand-replacing fires followed by hillslope erosion and sedimentation. These fires are usually attributed to increased stand density as a result of fire suppression, grazing and other land use, and are often considered uncharacteristic or unprecedented. Tree-ring records from the past 500 years indicate that before Euro-American settlement, frequent, low-severity fires maintained open stands. However, the pre-settlement period between about ad 1500 and ad 1900 was also generally colder than present, raising the possibility that rapid twentieth-century warming promoted recent catastrophic fires. Here we date fire-related sediment deposits in alluvial fans in central Idaho to reconstruct Holocene fire history in xeric ponderosa pine forests and examine links to climate. We find that colder periods experienced frequent low-severity fires, probably fuelled by increased understory growth. Warmer periods experienced severe droughts, stand-replacing fires and large debris-flow events that comprise a large component of long-term erosion and coincide with similar events in sub-alpine forests of Yellowstone National Park. Our results suggest that given the powerful influence of climate, restoration of processes typical of pre-settlement times may be difficult in a warmer future that promotes severe fires.  相似文献   
977.
针对内窥镜系统光学畸变问题,提出了医用电子内窥镜畸变实时校正系统的原理和硬件系统的实现.系统采用基于点阵样板的校正方法,拟合理想像与实际像的关系,完成像素点的几何位置校正和灰度校正;采用FP-GA作为控制中心,设计了I^2C串行总线通信,协调了解码器与编码器的同步控制,最终利用硬件实现了畸变实时校正功能.图像采样频率13.5MHz,帧频达25帧/s.  相似文献   
978.
979.
HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca2+ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca2+ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca2+ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004  相似文献   
980.
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号